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Blood M2-like Monocyte Polarization Is Associated with Calcific Plaque Phenotype in Stable Coronary Artery Disease: A Sub-Study of SMARTool Clinical Trial
Background: Atherosclerosis is a chronic inflammatory disease. The balance between pro- and anti-inflammatory factors, acting on the arterial wall, promotes less or more coronary plaque macro-calcification, respectively. We investigated the association between monocyte phenotypic polarization and CT...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945688/ https://www.ncbi.nlm.nih.gov/pubmed/35327367 http://dx.doi.org/10.3390/biomedicines10030565 |
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author | Sbrana, Silverio Cecchettini, Antonella Bastiani, Luca Di Giorgi, Nicoletta Mazzone, Annamaria Ceccherini, Elisa Vozzi, Federico Caselli, Chiara Neglia, Danilo Clemente, Alberto Scholte, Arthur J. H. A. Parodi, Oberdan Pelosi, Gualtiero Rocchiccioli, Silvia |
author_facet | Sbrana, Silverio Cecchettini, Antonella Bastiani, Luca Di Giorgi, Nicoletta Mazzone, Annamaria Ceccherini, Elisa Vozzi, Federico Caselli, Chiara Neglia, Danilo Clemente, Alberto Scholte, Arthur J. H. A. Parodi, Oberdan Pelosi, Gualtiero Rocchiccioli, Silvia |
author_sort | Sbrana, Silverio |
collection | PubMed |
description | Background: Atherosclerosis is a chronic inflammatory disease. The balance between pro- and anti-inflammatory factors, acting on the arterial wall, promotes less or more coronary plaque macro-calcification, respectively. We investigated the association between monocyte phenotypic polarization and CTCA-assessed plaque dense-calcium volume (DCV) in patients with stable coronary artery disease (CAD). Methods: In 55 patients, individual DCV component was assessed by quantitative CTCA and normalized to total plaque volume. Flow cytometry expression of CD14, CD16, CD18, CD11b, HLA-DR, CD163, CCR2, CCR5, CX3CR1 and CXCR4 was quantified. Adhesion molecules and cytokines were measured by ELISA. Results: DCV values were significantly associated, by multiple regression analysis, with the expression (RFI) of CCR5 (p = 0.04), CX3CR1 (p = 0.03), CCR2 (p = 0.02), CD163 (p = 0.005) on all monocytes, and with the phenotypic M2-like polarization ratio, RFI CCR5/CD11b (p = 0.01). A positive correlation with the increased expression of chemokines receptors CCR2, CCR5 and CX3CR1 on subsets Mon1 was also present. Among cytokines, the ratio between IL-10 and IL-6 was found to be strongly associated with DCV (p = 0.009). Conclusions: The association between DCV and M2-like phenotypic polarization of circulating monocytes indicates that plaque macro-calcification in stable CAD may be partly modulated by an anti-inflammatory monocyte functional state, as evidenced by cell membrane receptor patterns. |
format | Online Article Text |
id | pubmed-8945688 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89456882022-03-25 Blood M2-like Monocyte Polarization Is Associated with Calcific Plaque Phenotype in Stable Coronary Artery Disease: A Sub-Study of SMARTool Clinical Trial Sbrana, Silverio Cecchettini, Antonella Bastiani, Luca Di Giorgi, Nicoletta Mazzone, Annamaria Ceccherini, Elisa Vozzi, Federico Caselli, Chiara Neglia, Danilo Clemente, Alberto Scholte, Arthur J. H. A. Parodi, Oberdan Pelosi, Gualtiero Rocchiccioli, Silvia Biomedicines Article Background: Atherosclerosis is a chronic inflammatory disease. The balance between pro- and anti-inflammatory factors, acting on the arterial wall, promotes less or more coronary plaque macro-calcification, respectively. We investigated the association between monocyte phenotypic polarization and CTCA-assessed plaque dense-calcium volume (DCV) in patients with stable coronary artery disease (CAD). Methods: In 55 patients, individual DCV component was assessed by quantitative CTCA and normalized to total plaque volume. Flow cytometry expression of CD14, CD16, CD18, CD11b, HLA-DR, CD163, CCR2, CCR5, CX3CR1 and CXCR4 was quantified. Adhesion molecules and cytokines were measured by ELISA. Results: DCV values were significantly associated, by multiple regression analysis, with the expression (RFI) of CCR5 (p = 0.04), CX3CR1 (p = 0.03), CCR2 (p = 0.02), CD163 (p = 0.005) on all monocytes, and with the phenotypic M2-like polarization ratio, RFI CCR5/CD11b (p = 0.01). A positive correlation with the increased expression of chemokines receptors CCR2, CCR5 and CX3CR1 on subsets Mon1 was also present. Among cytokines, the ratio between IL-10 and IL-6 was found to be strongly associated with DCV (p = 0.009). Conclusions: The association between DCV and M2-like phenotypic polarization of circulating monocytes indicates that plaque macro-calcification in stable CAD may be partly modulated by an anti-inflammatory monocyte functional state, as evidenced by cell membrane receptor patterns. MDPI 2022-02-28 /pmc/articles/PMC8945688/ /pubmed/35327367 http://dx.doi.org/10.3390/biomedicines10030565 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sbrana, Silverio Cecchettini, Antonella Bastiani, Luca Di Giorgi, Nicoletta Mazzone, Annamaria Ceccherini, Elisa Vozzi, Federico Caselli, Chiara Neglia, Danilo Clemente, Alberto Scholte, Arthur J. H. A. Parodi, Oberdan Pelosi, Gualtiero Rocchiccioli, Silvia Blood M2-like Monocyte Polarization Is Associated with Calcific Plaque Phenotype in Stable Coronary Artery Disease: A Sub-Study of SMARTool Clinical Trial |
title | Blood M2-like Monocyte Polarization Is Associated with Calcific Plaque Phenotype in Stable Coronary Artery Disease: A Sub-Study of SMARTool Clinical Trial |
title_full | Blood M2-like Monocyte Polarization Is Associated with Calcific Plaque Phenotype in Stable Coronary Artery Disease: A Sub-Study of SMARTool Clinical Trial |
title_fullStr | Blood M2-like Monocyte Polarization Is Associated with Calcific Plaque Phenotype in Stable Coronary Artery Disease: A Sub-Study of SMARTool Clinical Trial |
title_full_unstemmed | Blood M2-like Monocyte Polarization Is Associated with Calcific Plaque Phenotype in Stable Coronary Artery Disease: A Sub-Study of SMARTool Clinical Trial |
title_short | Blood M2-like Monocyte Polarization Is Associated with Calcific Plaque Phenotype in Stable Coronary Artery Disease: A Sub-Study of SMARTool Clinical Trial |
title_sort | blood m2-like monocyte polarization is associated with calcific plaque phenotype in stable coronary artery disease: a sub-study of smartool clinical trial |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945688/ https://www.ncbi.nlm.nih.gov/pubmed/35327367 http://dx.doi.org/10.3390/biomedicines10030565 |
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