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Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium

The production of biodegradable and biocompatible materials such as polyhydroxyalkanoates (PHAs) from waste-derived volatile fatty acids (VFAs) is a promising approach towards implementing a circular bioeconomy. However, VFA solutions obtained via acidification of organic wastes are usually too dilu...

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Autores principales: Kacanski, Milos, Pucher, Lukas, Peral, Carlota, Dietrich, Thomas, Neureiter, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945770/
https://www.ncbi.nlm.nih.gov/pubmed/35324811
http://dx.doi.org/10.3390/bioengineering9030122
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author Kacanski, Milos
Pucher, Lukas
Peral, Carlota
Dietrich, Thomas
Neureiter, Markus
author_facet Kacanski, Milos
Pucher, Lukas
Peral, Carlota
Dietrich, Thomas
Neureiter, Markus
author_sort Kacanski, Milos
collection PubMed
description The production of biodegradable and biocompatible materials such as polyhydroxyalkanoates (PHAs) from waste-derived volatile fatty acids (VFAs) is a promising approach towards implementing a circular bioeconomy. However, VFA solutions obtained via acidification of organic wastes are usually too diluted for direct use in standard batch or fed-batch processes. To overcome these constraints, this study introduces a cell recycle fed-batch system using Bacillus megaterium uyuni S29 for poly(3-hydroxybutyrate) (P3HB) production from acetic acid. The concentrations of dry cell weight (DCW), P3HB, acetate, as well as nitrogen as the limiting substrate component, were monitored during the process. The produced polymer was characterized in terms of molecular weight and thermal properties after extraction with hypochlorite. The results show that an indirect pH-stat feeding regime successfully kept the strain fed without prompting inhibition, resulting in a dry cell weight concentration of up to 19.05 g/L containing 70.21% PHA. After appropriate adaptations the presented process could contribute to an efficient and sustainable production of biopolymers.
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spelling pubmed-89457702022-03-25 Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium Kacanski, Milos Pucher, Lukas Peral, Carlota Dietrich, Thomas Neureiter, Markus Bioengineering (Basel) Article The production of biodegradable and biocompatible materials such as polyhydroxyalkanoates (PHAs) from waste-derived volatile fatty acids (VFAs) is a promising approach towards implementing a circular bioeconomy. However, VFA solutions obtained via acidification of organic wastes are usually too diluted for direct use in standard batch or fed-batch processes. To overcome these constraints, this study introduces a cell recycle fed-batch system using Bacillus megaterium uyuni S29 for poly(3-hydroxybutyrate) (P3HB) production from acetic acid. The concentrations of dry cell weight (DCW), P3HB, acetate, as well as nitrogen as the limiting substrate component, were monitored during the process. The produced polymer was characterized in terms of molecular weight and thermal properties after extraction with hypochlorite. The results show that an indirect pH-stat feeding regime successfully kept the strain fed without prompting inhibition, resulting in a dry cell weight concentration of up to 19.05 g/L containing 70.21% PHA. After appropriate adaptations the presented process could contribute to an efficient and sustainable production of biopolymers. MDPI 2022-03-16 /pmc/articles/PMC8945770/ /pubmed/35324811 http://dx.doi.org/10.3390/bioengineering9030122 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kacanski, Milos
Pucher, Lukas
Peral, Carlota
Dietrich, Thomas
Neureiter, Markus
Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium
title Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium
title_full Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium
title_fullStr Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium
title_full_unstemmed Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium
title_short Cell Retention as a Viable Strategy for PHA Production from Diluted VFAs with Bacillus megaterium
title_sort cell retention as a viable strategy for pha production from diluted vfas with bacillus megaterium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945770/
https://www.ncbi.nlm.nih.gov/pubmed/35324811
http://dx.doi.org/10.3390/bioengineering9030122
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