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Expression Profile of CD157 Reveals Functional Heterogeneity of Capillaries in Human Dermal Skin

CD157 acts as a receptor, regulating leukocyte trafficking and the binding of extracellular matrix components. However, the expression pattern and the role of CD157 in human blood (BEC) and the lymphatic endothelial cells (LEC) of human dermal microvascular cells (HDMEC), remain elusive. We demonstr...

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Autores principales: Michalak-Micka, Katarzyna, Rütsche, Dominic, Johner, Lukas, Moehrlen, Ueli, Biedermann, Thomas, Klar, Agnes S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945771/
https://www.ncbi.nlm.nih.gov/pubmed/35327478
http://dx.doi.org/10.3390/biomedicines10030676
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author Michalak-Micka, Katarzyna
Rütsche, Dominic
Johner, Lukas
Moehrlen, Ueli
Biedermann, Thomas
Klar, Agnes S.
author_facet Michalak-Micka, Katarzyna
Rütsche, Dominic
Johner, Lukas
Moehrlen, Ueli
Biedermann, Thomas
Klar, Agnes S.
author_sort Michalak-Micka, Katarzyna
collection PubMed
description CD157 acts as a receptor, regulating leukocyte trafficking and the binding of extracellular matrix components. However, the expression pattern and the role of CD157 in human blood (BEC) and the lymphatic endothelial cells (LEC) of human dermal microvascular cells (HDMEC), remain elusive. We demonstrated constitutive expression of CD157 on BEC and LEC, in fetal and juvenile/adult skin, in situ, as well as in isolated HDMEC. Interestingly, CD157 epitopes were mostly localized on BEC, co-expressing high levels of CD31 (CD31(High)), as compared to CD31(Low) BEC, whereas the podoplanin expression level on LEC did not affect CD157. Cultured HDMEC exhibited significantly higher numbers of CD157-positive LEC, as compared to BEC. Interestingly, separated CD157(−) and CD157(+) HDMEC demonstrated no significant differences in clonal expansion in vitro, but they showed distinct expression levels of cell adhesion molecules, before and after cytokine stimulation in vitro. In particular, we proved the enhanced and specific adherence of CD11b-expressing human blood myeloid cells to CD157(+) HDMEC fraction, using an in vitro immune-binding assay. Indeed, CD157 was also involved in chemotaxis and adhesion of CD11b/c monocytes/neutrophils in prevascularized dermo–epidermal skin substitutes (vascDESS) in vivo. Thus, our data attribute specific roles to endothelial CD157, in the regulation of innate immunity during inflammation.
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spelling pubmed-89457712022-03-25 Expression Profile of CD157 Reveals Functional Heterogeneity of Capillaries in Human Dermal Skin Michalak-Micka, Katarzyna Rütsche, Dominic Johner, Lukas Moehrlen, Ueli Biedermann, Thomas Klar, Agnes S. Biomedicines Article CD157 acts as a receptor, regulating leukocyte trafficking and the binding of extracellular matrix components. However, the expression pattern and the role of CD157 in human blood (BEC) and the lymphatic endothelial cells (LEC) of human dermal microvascular cells (HDMEC), remain elusive. We demonstrated constitutive expression of CD157 on BEC and LEC, in fetal and juvenile/adult skin, in situ, as well as in isolated HDMEC. Interestingly, CD157 epitopes were mostly localized on BEC, co-expressing high levels of CD31 (CD31(High)), as compared to CD31(Low) BEC, whereas the podoplanin expression level on LEC did not affect CD157. Cultured HDMEC exhibited significantly higher numbers of CD157-positive LEC, as compared to BEC. Interestingly, separated CD157(−) and CD157(+) HDMEC demonstrated no significant differences in clonal expansion in vitro, but they showed distinct expression levels of cell adhesion molecules, before and after cytokine stimulation in vitro. In particular, we proved the enhanced and specific adherence of CD11b-expressing human blood myeloid cells to CD157(+) HDMEC fraction, using an in vitro immune-binding assay. Indeed, CD157 was also involved in chemotaxis and adhesion of CD11b/c monocytes/neutrophils in prevascularized dermo–epidermal skin substitutes (vascDESS) in vivo. Thus, our data attribute specific roles to endothelial CD157, in the regulation of innate immunity during inflammation. MDPI 2022-03-15 /pmc/articles/PMC8945771/ /pubmed/35327478 http://dx.doi.org/10.3390/biomedicines10030676 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Michalak-Micka, Katarzyna
Rütsche, Dominic
Johner, Lukas
Moehrlen, Ueli
Biedermann, Thomas
Klar, Agnes S.
Expression Profile of CD157 Reveals Functional Heterogeneity of Capillaries in Human Dermal Skin
title Expression Profile of CD157 Reveals Functional Heterogeneity of Capillaries in Human Dermal Skin
title_full Expression Profile of CD157 Reveals Functional Heterogeneity of Capillaries in Human Dermal Skin
title_fullStr Expression Profile of CD157 Reveals Functional Heterogeneity of Capillaries in Human Dermal Skin
title_full_unstemmed Expression Profile of CD157 Reveals Functional Heterogeneity of Capillaries in Human Dermal Skin
title_short Expression Profile of CD157 Reveals Functional Heterogeneity of Capillaries in Human Dermal Skin
title_sort expression profile of cd157 reveals functional heterogeneity of capillaries in human dermal skin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945771/
https://www.ncbi.nlm.nih.gov/pubmed/35327478
http://dx.doi.org/10.3390/biomedicines10030676
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