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Is Immuno-PCR Better than ELISA Test for Detection of Toxoplasma gondii IgG Antibody?

INTRODUCTION: IgG antibodies against T. gondii persist for years, and can act as a reliable serological biomarker for the diagnosis of previous exposure to this parasite. Hence, the current investigation was designed to compare diagnostic power of immuno-polymerase chain reaction (iPCR) and enzyme-l...

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Autores principales: Mardani-Kataki, Masoumeh, Beiromvand, Molouk, Teimoori, Ali, Amari, Afshin, Tavalla, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945868/
https://www.ncbi.nlm.nih.gov/pubmed/35332384
http://dx.doi.org/10.1007/s11686-022-00537-1
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author Mardani-Kataki, Masoumeh
Beiromvand, Molouk
Teimoori, Ali
Amari, Afshin
Tavalla, Mehdi
author_facet Mardani-Kataki, Masoumeh
Beiromvand, Molouk
Teimoori, Ali
Amari, Afshin
Tavalla, Mehdi
author_sort Mardani-Kataki, Masoumeh
collection PubMed
description INTRODUCTION: IgG antibodies against T. gondii persist for years, and can act as a reliable serological biomarker for the diagnosis of previous exposure to this parasite. Hence, the current investigation was designed to compare diagnostic power of immuno-polymerase chain reaction (iPCR) and enzyme-linked immunosorbent assay (ELISA) methods for detection of T. gondii IgG antibody. METHODS: Immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies against T. gondii were measured by the ELISA method in 81 participants. In addition, detection of acute and chronic toxoplasmosis was performed via the ELISA IgG avidity. The set-up of iPCR was carried out and then, serum IgG of subjects were detected using the iPCR method. RESULTS: Of 81 samples, 4 (4.9%) and 30 (37%) cases were be found positive for IgM and IgG against T. gondii in the ELISA method, respectively. Moreover, of 81 specimens, 42 (51.9%) and 39 (48.1%) samples had low-avidity IgG and high-avidity IgG by the IgG avidity kit, respectively. While, 59 (72.8%) of 81 samples were detected positive using the iPCR technique. Kappa (κ) value coefficient, between the iPCR and ELISA (for IgG) showed a strong agreement (0.360, p value < 0.001). A value of 0.25 I.U./ml for serum IgG [area under curve (AUC) = 0.720 (CI = 0.613–0.827); p = 0.002] was the cut-off value to differentiating between positive and negative toxoplasmosis (with sensitivity 66.0% and specificity 60.0%). CONCLUSION: Our findings indicated despite a strong agreement shown between iPCR and ELISA methods, the diagnostic power of iPCR technique was more sensitive than ELISA test for detection of T. gondii IgG antibody. However, more complementary investigations are widely needed in this regard.
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spelling pubmed-89458682022-03-25 Is Immuno-PCR Better than ELISA Test for Detection of Toxoplasma gondii IgG Antibody? Mardani-Kataki, Masoumeh Beiromvand, Molouk Teimoori, Ali Amari, Afshin Tavalla, Mehdi Acta Parasitol Original Paper INTRODUCTION: IgG antibodies against T. gondii persist for years, and can act as a reliable serological biomarker for the diagnosis of previous exposure to this parasite. Hence, the current investigation was designed to compare diagnostic power of immuno-polymerase chain reaction (iPCR) and enzyme-linked immunosorbent assay (ELISA) methods for detection of T. gondii IgG antibody. METHODS: Immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies against T. gondii were measured by the ELISA method in 81 participants. In addition, detection of acute and chronic toxoplasmosis was performed via the ELISA IgG avidity. The set-up of iPCR was carried out and then, serum IgG of subjects were detected using the iPCR method. RESULTS: Of 81 samples, 4 (4.9%) and 30 (37%) cases were be found positive for IgM and IgG against T. gondii in the ELISA method, respectively. Moreover, of 81 specimens, 42 (51.9%) and 39 (48.1%) samples had low-avidity IgG and high-avidity IgG by the IgG avidity kit, respectively. While, 59 (72.8%) of 81 samples were detected positive using the iPCR technique. Kappa (κ) value coefficient, between the iPCR and ELISA (for IgG) showed a strong agreement (0.360, p value < 0.001). A value of 0.25 I.U./ml for serum IgG [area under curve (AUC) = 0.720 (CI = 0.613–0.827); p = 0.002] was the cut-off value to differentiating between positive and negative toxoplasmosis (with sensitivity 66.0% and specificity 60.0%). CONCLUSION: Our findings indicated despite a strong agreement shown between iPCR and ELISA methods, the diagnostic power of iPCR technique was more sensitive than ELISA test for detection of T. gondii IgG antibody. However, more complementary investigations are widely needed in this regard. Springer International Publishing 2022-03-24 2022 /pmc/articles/PMC8945868/ /pubmed/35332384 http://dx.doi.org/10.1007/s11686-022-00537-1 Text en © This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Paper
Mardani-Kataki, Masoumeh
Beiromvand, Molouk
Teimoori, Ali
Amari, Afshin
Tavalla, Mehdi
Is Immuno-PCR Better than ELISA Test for Detection of Toxoplasma gondii IgG Antibody?
title Is Immuno-PCR Better than ELISA Test for Detection of Toxoplasma gondii IgG Antibody?
title_full Is Immuno-PCR Better than ELISA Test for Detection of Toxoplasma gondii IgG Antibody?
title_fullStr Is Immuno-PCR Better than ELISA Test for Detection of Toxoplasma gondii IgG Antibody?
title_full_unstemmed Is Immuno-PCR Better than ELISA Test for Detection of Toxoplasma gondii IgG Antibody?
title_short Is Immuno-PCR Better than ELISA Test for Detection of Toxoplasma gondii IgG Antibody?
title_sort is immuno-pcr better than elisa test for detection of toxoplasma gondii igg antibody?
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945868/
https://www.ncbi.nlm.nih.gov/pubmed/35332384
http://dx.doi.org/10.1007/s11686-022-00537-1
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