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Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis

Background: The aim of this study was to analyze possible interactions of different xenogeneic collagen membranes (CM) and platelet-rich fibrin (PRF). PH values were evaluated in the CM rehydration process with PRF, and their influence on angiogenesis was analyzed in vivo. Materials and Methods: Por...

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Autores principales: Blatt, Sebastian, Schröger, Saskia-Vanessa, Pabst, Andreas, Kämmerer, Peer W., Sagheb, Keyvan, Al-Nawas, Bilal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945896/
https://www.ncbi.nlm.nih.gov/pubmed/35327506
http://dx.doi.org/10.3390/biomedicines10030706
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author Blatt, Sebastian
Schröger, Saskia-Vanessa
Pabst, Andreas
Kämmerer, Peer W.
Sagheb, Keyvan
Al-Nawas, Bilal
author_facet Blatt, Sebastian
Schröger, Saskia-Vanessa
Pabst, Andreas
Kämmerer, Peer W.
Sagheb, Keyvan
Al-Nawas, Bilal
author_sort Blatt, Sebastian
collection PubMed
description Background: The aim of this study was to analyze possible interactions of different xenogeneic collagen membranes (CM) and platelet-rich fibrin (PRF). PH values were evaluated in the CM rehydration process with PRF, and their influence on angiogenesis was analyzed in vivo. Materials and Methods: Porcine (Bio-Gide(®), Geistlich)- and bovine-derived collagen membranes (Symbios(®), Dentsply Sirona) were biofunctionalized with PRF by plotting process. PRF in comparison to blood, saline and a puffer pH7 solution was analysed for pH-value changes in CM rehydration process in vitro. The yolk sac membrane (YSM) model was used to investigate pro-angiogenic effects of the combination of PRF and the respective CM in comparison to native pendant by vessel in-growth and branching points after 24, 48 and 72 h evaluated light-microscopically and by immunohistochemical staining (CD105, αSMA) in vivo. Results: Significantly higher pH values were found at all points in time in PRF alone and its combined variants with Bio-Gide(®) and Symbios(®) compared with pure native saline solution and pH 7 solution, as well as saline with Symbios(®) and Bio-Gide(®) (each p < 0.01). In the YSM, vessel number and branching points showed no significant differences at 24 and 48 h between all groups (each p > 0.05). For PRF alone, a significantly increased vessel number and branching points between 24 and 48 h (each p < 0.05) and between 24 and 72 h (each p < 0.05) was shown. After 72 h, CM in combination with PRF induced a statistically significant addition to vessels and branching points in comparison with native YSM (p < 0.01) but not vs. its native pendants (p > 0.05). Summary: PRF represents a promising alternative for CM rehydration to enhance CM vascularization.
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spelling pubmed-89458962022-03-25 Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis Blatt, Sebastian Schröger, Saskia-Vanessa Pabst, Andreas Kämmerer, Peer W. Sagheb, Keyvan Al-Nawas, Bilal Biomedicines Article Background: The aim of this study was to analyze possible interactions of different xenogeneic collagen membranes (CM) and platelet-rich fibrin (PRF). PH values were evaluated in the CM rehydration process with PRF, and their influence on angiogenesis was analyzed in vivo. Materials and Methods: Porcine (Bio-Gide(®), Geistlich)- and bovine-derived collagen membranes (Symbios(®), Dentsply Sirona) were biofunctionalized with PRF by plotting process. PRF in comparison to blood, saline and a puffer pH7 solution was analysed for pH-value changes in CM rehydration process in vitro. The yolk sac membrane (YSM) model was used to investigate pro-angiogenic effects of the combination of PRF and the respective CM in comparison to native pendant by vessel in-growth and branching points after 24, 48 and 72 h evaluated light-microscopically and by immunohistochemical staining (CD105, αSMA) in vivo. Results: Significantly higher pH values were found at all points in time in PRF alone and its combined variants with Bio-Gide(®) and Symbios(®) compared with pure native saline solution and pH 7 solution, as well as saline with Symbios(®) and Bio-Gide(®) (each p < 0.01). In the YSM, vessel number and branching points showed no significant differences at 24 and 48 h between all groups (each p > 0.05). For PRF alone, a significantly increased vessel number and branching points between 24 and 48 h (each p < 0.05) and between 24 and 72 h (each p < 0.05) was shown. After 72 h, CM in combination with PRF induced a statistically significant addition to vessels and branching points in comparison with native YSM (p < 0.01) but not vs. its native pendants (p > 0.05). Summary: PRF represents a promising alternative for CM rehydration to enhance CM vascularization. MDPI 2022-03-18 /pmc/articles/PMC8945896/ /pubmed/35327506 http://dx.doi.org/10.3390/biomedicines10030706 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Blatt, Sebastian
Schröger, Saskia-Vanessa
Pabst, Andreas
Kämmerer, Peer W.
Sagheb, Keyvan
Al-Nawas, Bilal
Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis
title Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis
title_full Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis
title_fullStr Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis
title_full_unstemmed Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis
title_short Biofunctionalization of Xenogeneic Collagen Membranes with Autologous Platelet Concentrate—Influence on Rehydration Protocol and Angiogenesis
title_sort biofunctionalization of xenogeneic collagen membranes with autologous platelet concentrate—influence on rehydration protocol and angiogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8945896/
https://www.ncbi.nlm.nih.gov/pubmed/35327506
http://dx.doi.org/10.3390/biomedicines10030706
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