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Identification and Characterization of Circular RNAs in Mammary Tissue from Holstein Cows at Early Lactation and Non-Lactation

In this study, circular RNAs (circRNAs) from Holstein cow mammary tissues were identified and compared between early lactation and non-lactation. After analysis, 10,684 circRNAs were identified, ranging from 48 to 99,406 bp, and the average size was 882 bp. The circRNAs were mainly distributed on ch...

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Autores principales: Liang, Yan, Gao, Qisong, Wang, Haiyang, Guo, Mengling, Arbab, Abdelaziz Adam Idriss, Nazar, Mudasir, Li, Mingxun, Yang, Zhangping, Karrow, Niel A., Mao, Yongjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946036/
https://www.ncbi.nlm.nih.gov/pubmed/35327670
http://dx.doi.org/10.3390/biom12030478
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author Liang, Yan
Gao, Qisong
Wang, Haiyang
Guo, Mengling
Arbab, Abdelaziz Adam Idriss
Nazar, Mudasir
Li, Mingxun
Yang, Zhangping
Karrow, Niel A.
Mao, Yongjiang
author_facet Liang, Yan
Gao, Qisong
Wang, Haiyang
Guo, Mengling
Arbab, Abdelaziz Adam Idriss
Nazar, Mudasir
Li, Mingxun
Yang, Zhangping
Karrow, Niel A.
Mao, Yongjiang
author_sort Liang, Yan
collection PubMed
description In this study, circular RNAs (circRNAs) from Holstein cow mammary tissues were identified and compared between early lactation and non-lactation. After analysis, 10,684 circRNAs were identified, ranging from 48 to 99,406 bp, and the average size was 882 bp. The circRNAs were mainly distributed on chromosomes 1 to 11, and 89.89% of the circRNAs belonged to sense-overlapping circRNA. The exons contained with circRNAs ranged from 1 to 47 and were concentrated from 1 to 5. Compared with the non-lactating cows, 87 circRNAs were significantly differentially expressed in the peak lactation cows. There were 68 upregulated circRNAs and 19 downregulated circRNAs. Enrichment analysis of circRNAs showed that GO analysis mainly focused on immune response, triglyceride transport, T cell receptor signaling pathway, etc. Pathway analysis mainly focused on cytokine-cytokine receptor interaction, T helper 17 cell differentiation, fatty acid biosynthesis, the JAK-STAT signaling pathway, etc. Specific primers were designed for two proximal ends of the circRNA junction sites to allow for PCR validation of four randomly selected circRNAs and carry out circRNA-miRNA interaction research. This study revealed the expression profile and characteristics of circRNAs in mammary tissue from Holstein cows at early lactation and non-lactation, thus providing rich information for the study of circRNA functions and mechanisms, as well as potential candidate miRNA genes for studying lactation in Holstein cows.
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spelling pubmed-89460362022-03-25 Identification and Characterization of Circular RNAs in Mammary Tissue from Holstein Cows at Early Lactation and Non-Lactation Liang, Yan Gao, Qisong Wang, Haiyang Guo, Mengling Arbab, Abdelaziz Adam Idriss Nazar, Mudasir Li, Mingxun Yang, Zhangping Karrow, Niel A. Mao, Yongjiang Biomolecules Article In this study, circular RNAs (circRNAs) from Holstein cow mammary tissues were identified and compared between early lactation and non-lactation. After analysis, 10,684 circRNAs were identified, ranging from 48 to 99,406 bp, and the average size was 882 bp. The circRNAs were mainly distributed on chromosomes 1 to 11, and 89.89% of the circRNAs belonged to sense-overlapping circRNA. The exons contained with circRNAs ranged from 1 to 47 and were concentrated from 1 to 5. Compared with the non-lactating cows, 87 circRNAs were significantly differentially expressed in the peak lactation cows. There were 68 upregulated circRNAs and 19 downregulated circRNAs. Enrichment analysis of circRNAs showed that GO analysis mainly focused on immune response, triglyceride transport, T cell receptor signaling pathway, etc. Pathway analysis mainly focused on cytokine-cytokine receptor interaction, T helper 17 cell differentiation, fatty acid biosynthesis, the JAK-STAT signaling pathway, etc. Specific primers were designed for two proximal ends of the circRNA junction sites to allow for PCR validation of four randomly selected circRNAs and carry out circRNA-miRNA interaction research. This study revealed the expression profile and characteristics of circRNAs in mammary tissue from Holstein cows at early lactation and non-lactation, thus providing rich information for the study of circRNA functions and mechanisms, as well as potential candidate miRNA genes for studying lactation in Holstein cows. MDPI 2022-03-21 /pmc/articles/PMC8946036/ /pubmed/35327670 http://dx.doi.org/10.3390/biom12030478 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liang, Yan
Gao, Qisong
Wang, Haiyang
Guo, Mengling
Arbab, Abdelaziz Adam Idriss
Nazar, Mudasir
Li, Mingxun
Yang, Zhangping
Karrow, Niel A.
Mao, Yongjiang
Identification and Characterization of Circular RNAs in Mammary Tissue from Holstein Cows at Early Lactation and Non-Lactation
title Identification and Characterization of Circular RNAs in Mammary Tissue from Holstein Cows at Early Lactation and Non-Lactation
title_full Identification and Characterization of Circular RNAs in Mammary Tissue from Holstein Cows at Early Lactation and Non-Lactation
title_fullStr Identification and Characterization of Circular RNAs in Mammary Tissue from Holstein Cows at Early Lactation and Non-Lactation
title_full_unstemmed Identification and Characterization of Circular RNAs in Mammary Tissue from Holstein Cows at Early Lactation and Non-Lactation
title_short Identification and Characterization of Circular RNAs in Mammary Tissue from Holstein Cows at Early Lactation and Non-Lactation
title_sort identification and characterization of circular rnas in mammary tissue from holstein cows at early lactation and non-lactation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946036/
https://www.ncbi.nlm.nih.gov/pubmed/35327670
http://dx.doi.org/10.3390/biom12030478
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