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A HepG2 Cell-Based Biosensor That Uses Stainless Steel Electrodes for Hepatotoxin Detection

Humans are frequently exposed to environmental hepatotoxins, which can lead to liver failure. Biosensors may be the best candidate for the detection of hepatotoxins because of their high sensitivity and specificity, convenience, time-saving, low cost, and extremely low detection limit. To investigat...

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Autores principales: Rozman, Martin, Štukovnik, Zala, Sušnik, Ajda, Pakseresht, Amirhossein, Hočevar, Matej, Drobne, Damjana, Bren, Urban
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946082/
https://www.ncbi.nlm.nih.gov/pubmed/35323430
http://dx.doi.org/10.3390/bios12030160
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author Rozman, Martin
Štukovnik, Zala
Sušnik, Ajda
Pakseresht, Amirhossein
Hočevar, Matej
Drobne, Damjana
Bren, Urban
author_facet Rozman, Martin
Štukovnik, Zala
Sušnik, Ajda
Pakseresht, Amirhossein
Hočevar, Matej
Drobne, Damjana
Bren, Urban
author_sort Rozman, Martin
collection PubMed
description Humans are frequently exposed to environmental hepatotoxins, which can lead to liver failure. Biosensors may be the best candidate for the detection of hepatotoxins because of their high sensitivity and specificity, convenience, time-saving, low cost, and extremely low detection limit. To investigate suitability of HepG2 cells for biosensor use, different methods of adhesion on stainless steel surfaces were investigated, with three groups of experiments performed in vitro. Cytotoxicity assays, which include the resazurin assay, the neutral red assay (NR), and the Coomassie Brilliant Blue (CBB) assay, were used to determine the viability of HepG2 cells exposed to various concentrations of aflatoxin B1 (AFB1) and isoniazid (INH) in parallel. The viability of the HepG2 cells on the stainless steel surface was quantitatively and qualitatively examined with different microscopy techniques. A simple cell-based electrochemical biosensor was developed by evaluating the viability of the HepG2 cells on the stainless steel surface when exposed to various concentrations of AFB1 and INH by using electrochemical impedance spectroscopy (EIS). The results showed that HepG2 cells can adhere to the metal surface and could be used as part of the biosensor to determine simple hepatotoxic samples.
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spelling pubmed-89460822022-03-25 A HepG2 Cell-Based Biosensor That Uses Stainless Steel Electrodes for Hepatotoxin Detection Rozman, Martin Štukovnik, Zala Sušnik, Ajda Pakseresht, Amirhossein Hočevar, Matej Drobne, Damjana Bren, Urban Biosensors (Basel) Article Humans are frequently exposed to environmental hepatotoxins, which can lead to liver failure. Biosensors may be the best candidate for the detection of hepatotoxins because of their high sensitivity and specificity, convenience, time-saving, low cost, and extremely low detection limit. To investigate suitability of HepG2 cells for biosensor use, different methods of adhesion on stainless steel surfaces were investigated, with three groups of experiments performed in vitro. Cytotoxicity assays, which include the resazurin assay, the neutral red assay (NR), and the Coomassie Brilliant Blue (CBB) assay, were used to determine the viability of HepG2 cells exposed to various concentrations of aflatoxin B1 (AFB1) and isoniazid (INH) in parallel. The viability of the HepG2 cells on the stainless steel surface was quantitatively and qualitatively examined with different microscopy techniques. A simple cell-based electrochemical biosensor was developed by evaluating the viability of the HepG2 cells on the stainless steel surface when exposed to various concentrations of AFB1 and INH by using electrochemical impedance spectroscopy (EIS). The results showed that HepG2 cells can adhere to the metal surface and could be used as part of the biosensor to determine simple hepatotoxic samples. MDPI 2022-03-04 /pmc/articles/PMC8946082/ /pubmed/35323430 http://dx.doi.org/10.3390/bios12030160 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rozman, Martin
Štukovnik, Zala
Sušnik, Ajda
Pakseresht, Amirhossein
Hočevar, Matej
Drobne, Damjana
Bren, Urban
A HepG2 Cell-Based Biosensor That Uses Stainless Steel Electrodes for Hepatotoxin Detection
title A HepG2 Cell-Based Biosensor That Uses Stainless Steel Electrodes for Hepatotoxin Detection
title_full A HepG2 Cell-Based Biosensor That Uses Stainless Steel Electrodes for Hepatotoxin Detection
title_fullStr A HepG2 Cell-Based Biosensor That Uses Stainless Steel Electrodes for Hepatotoxin Detection
title_full_unstemmed A HepG2 Cell-Based Biosensor That Uses Stainless Steel Electrodes for Hepatotoxin Detection
title_short A HepG2 Cell-Based Biosensor That Uses Stainless Steel Electrodes for Hepatotoxin Detection
title_sort hepg2 cell-based biosensor that uses stainless steel electrodes for hepatotoxin detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946082/
https://www.ncbi.nlm.nih.gov/pubmed/35323430
http://dx.doi.org/10.3390/bios12030160
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