Cargando…

HPLC-PDA-ESI-HRMS-Based Profiling of Secondary Metabolites of Rindera graeca Anatomical and Hairy Roots Treated with Drought and Cold Stress

To cope with environmental harmful conditions, plant cells developed adaptive strategy that involves production of a wide variety of complex secondary metabolites. The spectrum and quantity of biosynthesized compounds in specific plant species is determined by its genotype, tissue, developmental and...

Descripción completa

Detalles Bibliográficos
Autores principales: Naliwajski, Marcin Robert, Wileńska, Beata, Misicka, Aleksandra, Pietrosiuk, Agnieszka, Sykłowska-Baranek, Katarzyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946546/
https://www.ncbi.nlm.nih.gov/pubmed/35326382
http://dx.doi.org/10.3390/cells11060931
_version_ 1784674219569709056
author Naliwajski, Marcin Robert
Wileńska, Beata
Misicka, Aleksandra
Pietrosiuk, Agnieszka
Sykłowska-Baranek, Katarzyna
author_facet Naliwajski, Marcin Robert
Wileńska, Beata
Misicka, Aleksandra
Pietrosiuk, Agnieszka
Sykłowska-Baranek, Katarzyna
author_sort Naliwajski, Marcin Robert
collection PubMed
description To cope with environmental harmful conditions, plant cells developed adaptive strategy that involves production of a wide variety of complex secondary metabolites. The spectrum and quantity of biosynthesized compounds in specific plant species is determined by its genotype, tissue, developmental and physiological stage and environmental factors. This phenomenon was used to exploit the potential of anatomical and hairy root cultures of Rindera graeca to produce bioactive compounds. Cultivated in vitro roots were subjected to abiotic stresses i.e., drought or coldness. Next the extract profiling was performed using HPLC-PDA-ESI-HRMS method, as well quantitative determination of caffeic, rosmarinic and lithospermic B acids, that were present in all root extracts. Phenolic acids, flavonoids and iridoids represent the major groups of compounds detected in chemical profiles growing under various conditions roots. The highest number of phytochemicals was determined in roots subjected to coldness. Lithospermic B acid proved to be the most abundant compound in all investigated extracts. Among applied abiotic stress factors it was demonstrated that coldness affected to the most secondary metabolites production. The results of current study suggest that root cultures of R. graeca could serve as a new and abundant source of lithospermic B acid.
format Online
Article
Text
id pubmed-8946546
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89465462022-03-25 HPLC-PDA-ESI-HRMS-Based Profiling of Secondary Metabolites of Rindera graeca Anatomical and Hairy Roots Treated with Drought and Cold Stress Naliwajski, Marcin Robert Wileńska, Beata Misicka, Aleksandra Pietrosiuk, Agnieszka Sykłowska-Baranek, Katarzyna Cells Article To cope with environmental harmful conditions, plant cells developed adaptive strategy that involves production of a wide variety of complex secondary metabolites. The spectrum and quantity of biosynthesized compounds in specific plant species is determined by its genotype, tissue, developmental and physiological stage and environmental factors. This phenomenon was used to exploit the potential of anatomical and hairy root cultures of Rindera graeca to produce bioactive compounds. Cultivated in vitro roots were subjected to abiotic stresses i.e., drought or coldness. Next the extract profiling was performed using HPLC-PDA-ESI-HRMS method, as well quantitative determination of caffeic, rosmarinic and lithospermic B acids, that were present in all root extracts. Phenolic acids, flavonoids and iridoids represent the major groups of compounds detected in chemical profiles growing under various conditions roots. The highest number of phytochemicals was determined in roots subjected to coldness. Lithospermic B acid proved to be the most abundant compound in all investigated extracts. Among applied abiotic stress factors it was demonstrated that coldness affected to the most secondary metabolites production. The results of current study suggest that root cultures of R. graeca could serve as a new and abundant source of lithospermic B acid. MDPI 2022-03-08 /pmc/articles/PMC8946546/ /pubmed/35326382 http://dx.doi.org/10.3390/cells11060931 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Naliwajski, Marcin Robert
Wileńska, Beata
Misicka, Aleksandra
Pietrosiuk, Agnieszka
Sykłowska-Baranek, Katarzyna
HPLC-PDA-ESI-HRMS-Based Profiling of Secondary Metabolites of Rindera graeca Anatomical and Hairy Roots Treated with Drought and Cold Stress
title HPLC-PDA-ESI-HRMS-Based Profiling of Secondary Metabolites of Rindera graeca Anatomical and Hairy Roots Treated with Drought and Cold Stress
title_full HPLC-PDA-ESI-HRMS-Based Profiling of Secondary Metabolites of Rindera graeca Anatomical and Hairy Roots Treated with Drought and Cold Stress
title_fullStr HPLC-PDA-ESI-HRMS-Based Profiling of Secondary Metabolites of Rindera graeca Anatomical and Hairy Roots Treated with Drought and Cold Stress
title_full_unstemmed HPLC-PDA-ESI-HRMS-Based Profiling of Secondary Metabolites of Rindera graeca Anatomical and Hairy Roots Treated with Drought and Cold Stress
title_short HPLC-PDA-ESI-HRMS-Based Profiling of Secondary Metabolites of Rindera graeca Anatomical and Hairy Roots Treated with Drought and Cold Stress
title_sort hplc-pda-esi-hrms-based profiling of secondary metabolites of rindera graeca anatomical and hairy roots treated with drought and cold stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946546/
https://www.ncbi.nlm.nih.gov/pubmed/35326382
http://dx.doi.org/10.3390/cells11060931
work_keys_str_mv AT naliwajskimarcinrobert hplcpdaesihrmsbasedprofilingofsecondarymetabolitesofrinderagraecaanatomicalandhairyrootstreatedwithdroughtandcoldstress
AT wilenskabeata hplcpdaesihrmsbasedprofilingofsecondarymetabolitesofrinderagraecaanatomicalandhairyrootstreatedwithdroughtandcoldstress
AT misickaaleksandra hplcpdaesihrmsbasedprofilingofsecondarymetabolitesofrinderagraecaanatomicalandhairyrootstreatedwithdroughtandcoldstress
AT pietrosiukagnieszka hplcpdaesihrmsbasedprofilingofsecondarymetabolitesofrinderagraecaanatomicalandhairyrootstreatedwithdroughtandcoldstress
AT sykłowskabaranekkatarzyna hplcpdaesihrmsbasedprofilingofsecondarymetabolitesofrinderagraecaanatomicalandhairyrootstreatedwithdroughtandcoldstress