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The Influence of the Ventricular-Lumbar Gradient on Cerebrospinal Fluid Analysis in Serial Samples

Background: Cerebrospinal fluid (CSF) samples from patients with non-inflammatory neurological diseases are used for control groups in biomarker studies. Since large amounts of CSF are withdrawn, patients with idiopathic intracranial hypertension (IIH) or normal pressure hydrocephalus (NPH) are espe...

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Autores principales: Konen, Franz Felix, Lange, Peter, Wurster, Ulrich, Jendretzky, Konstantin Fritz, Gingele, Stefan, Möhn, Nora, Sühs, Kurt-Wolfram, Stangel, Martin, Skripuletz, Thomas, Schwenkenbecher, Philipp
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946585/
https://www.ncbi.nlm.nih.gov/pubmed/35326365
http://dx.doi.org/10.3390/brainsci12030410
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author Konen, Franz Felix
Lange, Peter
Wurster, Ulrich
Jendretzky, Konstantin Fritz
Gingele, Stefan
Möhn, Nora
Sühs, Kurt-Wolfram
Stangel, Martin
Skripuletz, Thomas
Schwenkenbecher, Philipp
author_facet Konen, Franz Felix
Lange, Peter
Wurster, Ulrich
Jendretzky, Konstantin Fritz
Gingele, Stefan
Möhn, Nora
Sühs, Kurt-Wolfram
Stangel, Martin
Skripuletz, Thomas
Schwenkenbecher, Philipp
author_sort Konen, Franz Felix
collection PubMed
description Background: Cerebrospinal fluid (CSF) samples from patients with non-inflammatory neurological diseases are used for control groups in biomarker studies. Since large amounts of CSF are withdrawn, patients with idiopathic intracranial hypertension (IIH) or normal pressure hydrocephalus (NPH) are especially suitable. The serially taken CSF portions are usually collected in different tubes. We aimed to investigate whether the later random choice of one of these tubes for CSF investigations might harbor the risk of different CSF protein findings due to the so-called ventriculo-lumbar CSF gradient. Methods: Patients with IIH (9) and NPH (7) were included. CSF was serially taken and collected in six tubes of 5 mL each. Concentrations and CSF-serum quotients of immunoglobulins, albumin and the virus-specific antibody index (AI) were determined in the first, fourth and sixth CSF fraction. Results: CSF immunoglobulin and albumin concentrations and CSF-serum protein quotients were significantly lower in the fourth and sixth CSF fraction compared with the first CSF fraction. Virus-specific AI did not significantly differ in the different CSF fractions. Conclusions: CSF protein analytics should be performed in the first CSF fraction in order to avoid different measurement results and achieve comparability within a control group and between different control and patient groups.
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spelling pubmed-89465852022-03-25 The Influence of the Ventricular-Lumbar Gradient on Cerebrospinal Fluid Analysis in Serial Samples Konen, Franz Felix Lange, Peter Wurster, Ulrich Jendretzky, Konstantin Fritz Gingele, Stefan Möhn, Nora Sühs, Kurt-Wolfram Stangel, Martin Skripuletz, Thomas Schwenkenbecher, Philipp Brain Sci Communication Background: Cerebrospinal fluid (CSF) samples from patients with non-inflammatory neurological diseases are used for control groups in biomarker studies. Since large amounts of CSF are withdrawn, patients with idiopathic intracranial hypertension (IIH) or normal pressure hydrocephalus (NPH) are especially suitable. The serially taken CSF portions are usually collected in different tubes. We aimed to investigate whether the later random choice of one of these tubes for CSF investigations might harbor the risk of different CSF protein findings due to the so-called ventriculo-lumbar CSF gradient. Methods: Patients with IIH (9) and NPH (7) were included. CSF was serially taken and collected in six tubes of 5 mL each. Concentrations and CSF-serum quotients of immunoglobulins, albumin and the virus-specific antibody index (AI) were determined in the first, fourth and sixth CSF fraction. Results: CSF immunoglobulin and albumin concentrations and CSF-serum protein quotients were significantly lower in the fourth and sixth CSF fraction compared with the first CSF fraction. Virus-specific AI did not significantly differ in the different CSF fractions. Conclusions: CSF protein analytics should be performed in the first CSF fraction in order to avoid different measurement results and achieve comparability within a control group and between different control and patient groups. MDPI 2022-03-20 /pmc/articles/PMC8946585/ /pubmed/35326365 http://dx.doi.org/10.3390/brainsci12030410 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Konen, Franz Felix
Lange, Peter
Wurster, Ulrich
Jendretzky, Konstantin Fritz
Gingele, Stefan
Möhn, Nora
Sühs, Kurt-Wolfram
Stangel, Martin
Skripuletz, Thomas
Schwenkenbecher, Philipp
The Influence of the Ventricular-Lumbar Gradient on Cerebrospinal Fluid Analysis in Serial Samples
title The Influence of the Ventricular-Lumbar Gradient on Cerebrospinal Fluid Analysis in Serial Samples
title_full The Influence of the Ventricular-Lumbar Gradient on Cerebrospinal Fluid Analysis in Serial Samples
title_fullStr The Influence of the Ventricular-Lumbar Gradient on Cerebrospinal Fluid Analysis in Serial Samples
title_full_unstemmed The Influence of the Ventricular-Lumbar Gradient on Cerebrospinal Fluid Analysis in Serial Samples
title_short The Influence of the Ventricular-Lumbar Gradient on Cerebrospinal Fluid Analysis in Serial Samples
title_sort influence of the ventricular-lumbar gradient on cerebrospinal fluid analysis in serial samples
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946585/
https://www.ncbi.nlm.nih.gov/pubmed/35326365
http://dx.doi.org/10.3390/brainsci12030410
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