Cargando…

Defining the Protease and Protease Inhibitor (P/PI) Proteomes of Healthy and Diseased Human Skin by Modified Systematic Review

Proteases and protease inhibitors (P/PIs) are involved in many biological processes in human skin, yet often only specific families or related groups of P/PIs are investigated. Proteomics approaches, such as mass spectrometry, can define proteome signatures (including P/PIs) in tissues; however, the...

Descripción completa

Detalles Bibliográficos
Autores principales: Stewart-McGuinness, Callum, Platt, Christopher I., Ozols, Matiss, Goh, Brian, Griffiths, Tamara W., Sherratt, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946613/
https://www.ncbi.nlm.nih.gov/pubmed/35327667
http://dx.doi.org/10.3390/biom12030475
_version_ 1784674235917008896
author Stewart-McGuinness, Callum
Platt, Christopher I.
Ozols, Matiss
Goh, Brian
Griffiths, Tamara W.
Sherratt, Michael J.
author_facet Stewart-McGuinness, Callum
Platt, Christopher I.
Ozols, Matiss
Goh, Brian
Griffiths, Tamara W.
Sherratt, Michael J.
author_sort Stewart-McGuinness, Callum
collection PubMed
description Proteases and protease inhibitors (P/PIs) are involved in many biological processes in human skin, yet often only specific families or related groups of P/PIs are investigated. Proteomics approaches, such as mass spectrometry, can define proteome signatures (including P/PIs) in tissues; however, they struggle to detect low-abundance proteins. To overcome these issues, we aimed to produce a comprehensive proteome of all P/PIs present in normal and diseased human skin, in vivo, by carrying out a modified systematic review using a list of P/PIs from MEROPS and combining this with key search terms in Web of Science. Resulting articles were manually reviewed against inclusion/exclusion criteria and a dataset constructed. This study identified 111 proteases and 77 protease inhibitors in human skin, comprising the serine, metallo-, cysteine and aspartic acid catalytic families of proteases. P/PIs showing no evidence of catalytic activity or protease inhibition, were designated non-peptidase homologs (NPH), and no reported protease inhibitory activity (NRPIA), respectively. MMP9 and TIMP1 were the most frequently published P/PIs and were reported in normal skin and most skin disease groups. Normal skin and diseased skin showed significant overlap with respect to P/PI profile; however, MMP23 was identified in several skin disease groups, but was absent in normal skin. The catalytic profile of P/PIs in wounds, scars and solar elastosis was distinct from normal skin, suggesting that a different group of P/PIs is responsible for disease progression. In conclusion, this study uses a novel approach to provide a comprehensive inventory of P/PIs in normal and diseased human skin reported in our database. The database may be used to determine either which P/PIs are present in specific diseases or which diseases individual P/PIs may influence.
format Online
Article
Text
id pubmed-8946613
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89466132022-03-25 Defining the Protease and Protease Inhibitor (P/PI) Proteomes of Healthy and Diseased Human Skin by Modified Systematic Review Stewart-McGuinness, Callum Platt, Christopher I. Ozols, Matiss Goh, Brian Griffiths, Tamara W. Sherratt, Michael J. Biomolecules Article Proteases and protease inhibitors (P/PIs) are involved in many biological processes in human skin, yet often only specific families or related groups of P/PIs are investigated. Proteomics approaches, such as mass spectrometry, can define proteome signatures (including P/PIs) in tissues; however, they struggle to detect low-abundance proteins. To overcome these issues, we aimed to produce a comprehensive proteome of all P/PIs present in normal and diseased human skin, in vivo, by carrying out a modified systematic review using a list of P/PIs from MEROPS and combining this with key search terms in Web of Science. Resulting articles were manually reviewed against inclusion/exclusion criteria and a dataset constructed. This study identified 111 proteases and 77 protease inhibitors in human skin, comprising the serine, metallo-, cysteine and aspartic acid catalytic families of proteases. P/PIs showing no evidence of catalytic activity or protease inhibition, were designated non-peptidase homologs (NPH), and no reported protease inhibitory activity (NRPIA), respectively. MMP9 and TIMP1 were the most frequently published P/PIs and were reported in normal skin and most skin disease groups. Normal skin and diseased skin showed significant overlap with respect to P/PI profile; however, MMP23 was identified in several skin disease groups, but was absent in normal skin. The catalytic profile of P/PIs in wounds, scars and solar elastosis was distinct from normal skin, suggesting that a different group of P/PIs is responsible for disease progression. In conclusion, this study uses a novel approach to provide a comprehensive inventory of P/PIs in normal and diseased human skin reported in our database. The database may be used to determine either which P/PIs are present in specific diseases or which diseases individual P/PIs may influence. MDPI 2022-03-20 /pmc/articles/PMC8946613/ /pubmed/35327667 http://dx.doi.org/10.3390/biom12030475 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stewart-McGuinness, Callum
Platt, Christopher I.
Ozols, Matiss
Goh, Brian
Griffiths, Tamara W.
Sherratt, Michael J.
Defining the Protease and Protease Inhibitor (P/PI) Proteomes of Healthy and Diseased Human Skin by Modified Systematic Review
title Defining the Protease and Protease Inhibitor (P/PI) Proteomes of Healthy and Diseased Human Skin by Modified Systematic Review
title_full Defining the Protease and Protease Inhibitor (P/PI) Proteomes of Healthy and Diseased Human Skin by Modified Systematic Review
title_fullStr Defining the Protease and Protease Inhibitor (P/PI) Proteomes of Healthy and Diseased Human Skin by Modified Systematic Review
title_full_unstemmed Defining the Protease and Protease Inhibitor (P/PI) Proteomes of Healthy and Diseased Human Skin by Modified Systematic Review
title_short Defining the Protease and Protease Inhibitor (P/PI) Proteomes of Healthy and Diseased Human Skin by Modified Systematic Review
title_sort defining the protease and protease inhibitor (p/pi) proteomes of healthy and diseased human skin by modified systematic review
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946613/
https://www.ncbi.nlm.nih.gov/pubmed/35327667
http://dx.doi.org/10.3390/biom12030475
work_keys_str_mv AT stewartmcguinnesscallum definingtheproteaseandproteaseinhibitorppiproteomesofhealthyanddiseasedhumanskinbymodifiedsystematicreview
AT plattchristopheri definingtheproteaseandproteaseinhibitorppiproteomesofhealthyanddiseasedhumanskinbymodifiedsystematicreview
AT ozolsmatiss definingtheproteaseandproteaseinhibitorppiproteomesofhealthyanddiseasedhumanskinbymodifiedsystematicreview
AT gohbrian definingtheproteaseandproteaseinhibitorppiproteomesofhealthyanddiseasedhumanskinbymodifiedsystematicreview
AT griffithstamaraw definingtheproteaseandproteaseinhibitorppiproteomesofhealthyanddiseasedhumanskinbymodifiedsystematicreview
AT sherrattmichaelj definingtheproteaseandproteaseinhibitorppiproteomesofhealthyanddiseasedhumanskinbymodifiedsystematicreview