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Deep-Learning-Aided Detection of Mycobacteria in Pathology Specimens Increases the Sensitivity in Early Diagnosis of Pulmonary Tuberculosis Compared with Bacteriology Tests

The histopathological diagnosis of mycobacterial infection may be improved by a comprehensive analysis using artificial intelligence. Two autopsy cases of pulmonary tuberculosis, and forty biopsy cases of undetected acid-fast bacilli (AFB) were used to train AI (convolutional neural network), and co...

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Autores principales: Zaizen, Yoshiaki, Kanahori, Yuki, Ishijima, Sousuke, Kitamura, Yuka, Yoon, Han-Seung, Ozasa, Mutsumi, Mukae, Hiroshi, Bychkov, Andrey, Hoshino, Tomoaki, Fukuoka, Junya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946921/
https://www.ncbi.nlm.nih.gov/pubmed/35328262
http://dx.doi.org/10.3390/diagnostics12030709
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author Zaizen, Yoshiaki
Kanahori, Yuki
Ishijima, Sousuke
Kitamura, Yuka
Yoon, Han-Seung
Ozasa, Mutsumi
Mukae, Hiroshi
Bychkov, Andrey
Hoshino, Tomoaki
Fukuoka, Junya
author_facet Zaizen, Yoshiaki
Kanahori, Yuki
Ishijima, Sousuke
Kitamura, Yuka
Yoon, Han-Seung
Ozasa, Mutsumi
Mukae, Hiroshi
Bychkov, Andrey
Hoshino, Tomoaki
Fukuoka, Junya
author_sort Zaizen, Yoshiaki
collection PubMed
description The histopathological diagnosis of mycobacterial infection may be improved by a comprehensive analysis using artificial intelligence. Two autopsy cases of pulmonary tuberculosis, and forty biopsy cases of undetected acid-fast bacilli (AFB) were used to train AI (convolutional neural network), and construct an AI to support AFB detection. Forty-two patients underwent bronchoscopy, and were evaluated using AI-supported pathology to detect AFB. The AI-supported pathology diagnosis was compared with bacteriology diagnosis from bronchial lavage fluid and the final definitive diagnosis of mycobacteriosis. Among the 16 patients with mycobacteriosis, bacteriology was positive in 9 patients (56%). Two patients (13%) were positive for AFB without AI assistance, whereas AI-supported pathology identified eleven positive patients (69%). When limited to tuberculosis, AI-supported pathology had significantly higher sensitivity compared with bacteriology (86% vs. 29%, p = 0.046). Seven patients diagnosed with mycobacteriosis had no consolidation or cavitary shadows in computed tomography; the sensitivity of bacteriology and AI-supported pathology was 29% and 86%, respectively (p = 0.046). The specificity of AI-supported pathology was 100% in this study. AI-supported pathology may be more sensitive than bacteriological tests for detecting AFB in samples collected via bronchoscopy.
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spelling pubmed-89469212022-03-25 Deep-Learning-Aided Detection of Mycobacteria in Pathology Specimens Increases the Sensitivity in Early Diagnosis of Pulmonary Tuberculosis Compared with Bacteriology Tests Zaizen, Yoshiaki Kanahori, Yuki Ishijima, Sousuke Kitamura, Yuka Yoon, Han-Seung Ozasa, Mutsumi Mukae, Hiroshi Bychkov, Andrey Hoshino, Tomoaki Fukuoka, Junya Diagnostics (Basel) Article The histopathological diagnosis of mycobacterial infection may be improved by a comprehensive analysis using artificial intelligence. Two autopsy cases of pulmonary tuberculosis, and forty biopsy cases of undetected acid-fast bacilli (AFB) were used to train AI (convolutional neural network), and construct an AI to support AFB detection. Forty-two patients underwent bronchoscopy, and were evaluated using AI-supported pathology to detect AFB. The AI-supported pathology diagnosis was compared with bacteriology diagnosis from bronchial lavage fluid and the final definitive diagnosis of mycobacteriosis. Among the 16 patients with mycobacteriosis, bacteriology was positive in 9 patients (56%). Two patients (13%) were positive for AFB without AI assistance, whereas AI-supported pathology identified eleven positive patients (69%). When limited to tuberculosis, AI-supported pathology had significantly higher sensitivity compared with bacteriology (86% vs. 29%, p = 0.046). Seven patients diagnosed with mycobacteriosis had no consolidation or cavitary shadows in computed tomography; the sensitivity of bacteriology and AI-supported pathology was 29% and 86%, respectively (p = 0.046). The specificity of AI-supported pathology was 100% in this study. AI-supported pathology may be more sensitive than bacteriological tests for detecting AFB in samples collected via bronchoscopy. MDPI 2022-03-14 /pmc/articles/PMC8946921/ /pubmed/35328262 http://dx.doi.org/10.3390/diagnostics12030709 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zaizen, Yoshiaki
Kanahori, Yuki
Ishijima, Sousuke
Kitamura, Yuka
Yoon, Han-Seung
Ozasa, Mutsumi
Mukae, Hiroshi
Bychkov, Andrey
Hoshino, Tomoaki
Fukuoka, Junya
Deep-Learning-Aided Detection of Mycobacteria in Pathology Specimens Increases the Sensitivity in Early Diagnosis of Pulmonary Tuberculosis Compared with Bacteriology Tests
title Deep-Learning-Aided Detection of Mycobacteria in Pathology Specimens Increases the Sensitivity in Early Diagnosis of Pulmonary Tuberculosis Compared with Bacteriology Tests
title_full Deep-Learning-Aided Detection of Mycobacteria in Pathology Specimens Increases the Sensitivity in Early Diagnosis of Pulmonary Tuberculosis Compared with Bacteriology Tests
title_fullStr Deep-Learning-Aided Detection of Mycobacteria in Pathology Specimens Increases the Sensitivity in Early Diagnosis of Pulmonary Tuberculosis Compared with Bacteriology Tests
title_full_unstemmed Deep-Learning-Aided Detection of Mycobacteria in Pathology Specimens Increases the Sensitivity in Early Diagnosis of Pulmonary Tuberculosis Compared with Bacteriology Tests
title_short Deep-Learning-Aided Detection of Mycobacteria in Pathology Specimens Increases the Sensitivity in Early Diagnosis of Pulmonary Tuberculosis Compared with Bacteriology Tests
title_sort deep-learning-aided detection of mycobacteria in pathology specimens increases the sensitivity in early diagnosis of pulmonary tuberculosis compared with bacteriology tests
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946921/
https://www.ncbi.nlm.nih.gov/pubmed/35328262
http://dx.doi.org/10.3390/diagnostics12030709
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