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miR-126 Decreases Proliferation and Mammosphere Formation of MCF-7 and Predicts Prognosis of ER+ Breast Cancer

Breast cancer (BC) is a major health burden that affects over one million women each year. It is the most prevalent cancer in women and the number one cancer killer of them worldwide. Of all BC subtypes, estrogen receptor-positive (ER+) BC is the most commonly diagnosed. The objective of this study...

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Autores principales: Msheik, Zahraa S., Nassar, Farah J., Chamandi, Ghada, Itani, Abdul Rahman, Gadaleta, Emanuala, Chalala, Claude, Alwan, Nisreen, Nasr, Rihab R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946945/
https://www.ncbi.nlm.nih.gov/pubmed/35328298
http://dx.doi.org/10.3390/diagnostics12030745
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author Msheik, Zahraa S.
Nassar, Farah J.
Chamandi, Ghada
Itani, Abdul Rahman
Gadaleta, Emanuala
Chalala, Claude
Alwan, Nisreen
Nasr, Rihab R.
author_facet Msheik, Zahraa S.
Nassar, Farah J.
Chamandi, Ghada
Itani, Abdul Rahman
Gadaleta, Emanuala
Chalala, Claude
Alwan, Nisreen
Nasr, Rihab R.
author_sort Msheik, Zahraa S.
collection PubMed
description Breast cancer (BC) is a major health burden that affects over one million women each year. It is the most prevalent cancer in women and the number one cancer killer of them worldwide. Of all BC subtypes, estrogen receptor-positive (ER+) BC is the most commonly diagnosed. The objective of this study is to investigate the contribution of miR-126 in the tumorigenesis of ER+ BC. miR-126 was downregulated in ER+ BC tissues from young breast cancer patients, as shown through miRNA microarray analysis and RT-qPCR. Subsequently, the effect of the modulation of miR-126 levels on the proliferation, cell cycle progression, and spheres formation of the ER+ BC cell line, MCF-7, was assessed by MTT assay, PI analysis, and mammosphere formation assay, respectively. miR-126 overexpression significantly decreased MCF-7 proliferation and mammosphere-forming ability, but did not affect cell cycle progression. Then, in silico analysis determined SLC7A5, PLXNB2, CRK, PLK2, SPRED1, and IRS1 as potential targets of miR-126. RT-qPCR data showed that miR-126 overexpression significantly downregulated SLC7A5 and PLXNB2 mRNA levels in MCF-7. Finally, in silico survival analysis showed that high expression of miR-126 or low expression of SLC7A5 correlated with better overall survival (OS) of ER+ BC patients. Overall, our study suggests that miR-126 might play a tumor suppressor role in ER+ BC. miR-126 and SLC7A5 might also be considered potential prognostic biomarkers in ER+ BC.
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spelling pubmed-89469452022-03-25 miR-126 Decreases Proliferation and Mammosphere Formation of MCF-7 and Predicts Prognosis of ER+ Breast Cancer Msheik, Zahraa S. Nassar, Farah J. Chamandi, Ghada Itani, Abdul Rahman Gadaleta, Emanuala Chalala, Claude Alwan, Nisreen Nasr, Rihab R. Diagnostics (Basel) Article Breast cancer (BC) is a major health burden that affects over one million women each year. It is the most prevalent cancer in women and the number one cancer killer of them worldwide. Of all BC subtypes, estrogen receptor-positive (ER+) BC is the most commonly diagnosed. The objective of this study is to investigate the contribution of miR-126 in the tumorigenesis of ER+ BC. miR-126 was downregulated in ER+ BC tissues from young breast cancer patients, as shown through miRNA microarray analysis and RT-qPCR. Subsequently, the effect of the modulation of miR-126 levels on the proliferation, cell cycle progression, and spheres formation of the ER+ BC cell line, MCF-7, was assessed by MTT assay, PI analysis, and mammosphere formation assay, respectively. miR-126 overexpression significantly decreased MCF-7 proliferation and mammosphere-forming ability, but did not affect cell cycle progression. Then, in silico analysis determined SLC7A5, PLXNB2, CRK, PLK2, SPRED1, and IRS1 as potential targets of miR-126. RT-qPCR data showed that miR-126 overexpression significantly downregulated SLC7A5 and PLXNB2 mRNA levels in MCF-7. Finally, in silico survival analysis showed that high expression of miR-126 or low expression of SLC7A5 correlated with better overall survival (OS) of ER+ BC patients. Overall, our study suggests that miR-126 might play a tumor suppressor role in ER+ BC. miR-126 and SLC7A5 might also be considered potential prognostic biomarkers in ER+ BC. MDPI 2022-03-18 /pmc/articles/PMC8946945/ /pubmed/35328298 http://dx.doi.org/10.3390/diagnostics12030745 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Msheik, Zahraa S.
Nassar, Farah J.
Chamandi, Ghada
Itani, Abdul Rahman
Gadaleta, Emanuala
Chalala, Claude
Alwan, Nisreen
Nasr, Rihab R.
miR-126 Decreases Proliferation and Mammosphere Formation of MCF-7 and Predicts Prognosis of ER+ Breast Cancer
title miR-126 Decreases Proliferation and Mammosphere Formation of MCF-7 and Predicts Prognosis of ER+ Breast Cancer
title_full miR-126 Decreases Proliferation and Mammosphere Formation of MCF-7 and Predicts Prognosis of ER+ Breast Cancer
title_fullStr miR-126 Decreases Proliferation and Mammosphere Formation of MCF-7 and Predicts Prognosis of ER+ Breast Cancer
title_full_unstemmed miR-126 Decreases Proliferation and Mammosphere Formation of MCF-7 and Predicts Prognosis of ER+ Breast Cancer
title_short miR-126 Decreases Proliferation and Mammosphere Formation of MCF-7 and Predicts Prognosis of ER+ Breast Cancer
title_sort mir-126 decreases proliferation and mammosphere formation of mcf-7 and predicts prognosis of er+ breast cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8946945/
https://www.ncbi.nlm.nih.gov/pubmed/35328298
http://dx.doi.org/10.3390/diagnostics12030745
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