Cargando…

Tremella fuciformis Crude Polysaccharides Attenuates Steatosis and Suppresses Inflammation in Diet-Induced NAFLD Mice

Nonalcoholic fatty liver disease (NAFLD) is a chronic liver disorder characterized by an enhanced accumulation of lipids, which affects around 40% of the world’s population. The T. fuciformis fungus possesses immunomodulatory activity and other beneficial properties that may alleviate steatosis thro...

Descripción completa

Detalles Bibliográficos
Autores principales: Khan, Tariq Jamal, Xu, Xiaofei, Xie, Xiaoling, Dai, Ximing, Sun, Pingnan, Xie, Qingdong, Zhou, Xiaoling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8947202/
https://www.ncbi.nlm.nih.gov/pubmed/35723304
http://dx.doi.org/10.3390/cimb44030081
_version_ 1784674382759591936
author Khan, Tariq Jamal
Xu, Xiaofei
Xie, Xiaoling
Dai, Ximing
Sun, Pingnan
Xie, Qingdong
Zhou, Xiaoling
author_facet Khan, Tariq Jamal
Xu, Xiaofei
Xie, Xiaoling
Dai, Ximing
Sun, Pingnan
Xie, Qingdong
Zhou, Xiaoling
author_sort Khan, Tariq Jamal
collection PubMed
description Nonalcoholic fatty liver disease (NAFLD) is a chronic liver disorder characterized by an enhanced accumulation of lipids, which affects around 40% of the world’s population. The T. fuciformis fungus possesses immunomodulatory activity and other beneficial properties that may alleviate steatosis through a different mechanism. The present study was designed to evaluate the effect T. fuciformis crude polysaccharides (TFCP) on inflammatory and lipid metabolism gene expression, oxidative stress, and lipid profile. Mice were divided into groups receiving (a) a normal chow diet (NCD), (b) a methionine–choline-deficient (MCD) diet, and (c) a MCD diet with TFCP. Liver histopathology was performed, and the hepatic gene expression levels were estimated using qRT-PCR. The lipid profiles, ALT, AST, and efficient oxidative enzymes were analyzed using ELISA. The TFCP administration in the MCD-fed mice suppressed hepatic lipid accumulation, lipid metabolism-associated genes (HMGCR, FABP, SREBP, ACC, and FAS), and inflammation-associated genes (IL-1β, TLR4, TNF-α, and IL-6) whilst enhancing the expression of HNF4α genes. TFCP mitigated against oxidative stress and normalized healthy lipid profiles. These results highlighted that TFCP prevents NAFLD through the inhibition of oxidative stress and inflammation, suggesting TFCP would potentially be an effective therapeutic agent against NAFLD progression.
format Online
Article
Text
id pubmed-8947202
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89472022022-06-04 Tremella fuciformis Crude Polysaccharides Attenuates Steatosis and Suppresses Inflammation in Diet-Induced NAFLD Mice Khan, Tariq Jamal Xu, Xiaofei Xie, Xiaoling Dai, Ximing Sun, Pingnan Xie, Qingdong Zhou, Xiaoling Curr Issues Mol Biol Article Nonalcoholic fatty liver disease (NAFLD) is a chronic liver disorder characterized by an enhanced accumulation of lipids, which affects around 40% of the world’s population. The T. fuciformis fungus possesses immunomodulatory activity and other beneficial properties that may alleviate steatosis through a different mechanism. The present study was designed to evaluate the effect T. fuciformis crude polysaccharides (TFCP) on inflammatory and lipid metabolism gene expression, oxidative stress, and lipid profile. Mice were divided into groups receiving (a) a normal chow diet (NCD), (b) a methionine–choline-deficient (MCD) diet, and (c) a MCD diet with TFCP. Liver histopathology was performed, and the hepatic gene expression levels were estimated using qRT-PCR. The lipid profiles, ALT, AST, and efficient oxidative enzymes were analyzed using ELISA. The TFCP administration in the MCD-fed mice suppressed hepatic lipid accumulation, lipid metabolism-associated genes (HMGCR, FABP, SREBP, ACC, and FAS), and inflammation-associated genes (IL-1β, TLR4, TNF-α, and IL-6) whilst enhancing the expression of HNF4α genes. TFCP mitigated against oxidative stress and normalized healthy lipid profiles. These results highlighted that TFCP prevents NAFLD through the inhibition of oxidative stress and inflammation, suggesting TFCP would potentially be an effective therapeutic agent against NAFLD progression. MDPI 2022-03-03 /pmc/articles/PMC8947202/ /pubmed/35723304 http://dx.doi.org/10.3390/cimb44030081 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khan, Tariq Jamal
Xu, Xiaofei
Xie, Xiaoling
Dai, Ximing
Sun, Pingnan
Xie, Qingdong
Zhou, Xiaoling
Tremella fuciformis Crude Polysaccharides Attenuates Steatosis and Suppresses Inflammation in Diet-Induced NAFLD Mice
title Tremella fuciformis Crude Polysaccharides Attenuates Steatosis and Suppresses Inflammation in Diet-Induced NAFLD Mice
title_full Tremella fuciformis Crude Polysaccharides Attenuates Steatosis and Suppresses Inflammation in Diet-Induced NAFLD Mice
title_fullStr Tremella fuciformis Crude Polysaccharides Attenuates Steatosis and Suppresses Inflammation in Diet-Induced NAFLD Mice
title_full_unstemmed Tremella fuciformis Crude Polysaccharides Attenuates Steatosis and Suppresses Inflammation in Diet-Induced NAFLD Mice
title_short Tremella fuciformis Crude Polysaccharides Attenuates Steatosis and Suppresses Inflammation in Diet-Induced NAFLD Mice
title_sort tremella fuciformis crude polysaccharides attenuates steatosis and suppresses inflammation in diet-induced nafld mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8947202/
https://www.ncbi.nlm.nih.gov/pubmed/35723304
http://dx.doi.org/10.3390/cimb44030081
work_keys_str_mv AT khantariqjamal tremellafuciformiscrudepolysaccharidesattenuatessteatosisandsuppressesinflammationindietinducednafldmice
AT xuxiaofei tremellafuciformiscrudepolysaccharidesattenuatessteatosisandsuppressesinflammationindietinducednafldmice
AT xiexiaoling tremellafuciformiscrudepolysaccharidesattenuatessteatosisandsuppressesinflammationindietinducednafldmice
AT daiximing tremellafuciformiscrudepolysaccharidesattenuatessteatosisandsuppressesinflammationindietinducednafldmice
AT sunpingnan tremellafuciformiscrudepolysaccharidesattenuatessteatosisandsuppressesinflammationindietinducednafldmice
AT xieqingdong tremellafuciformiscrudepolysaccharidesattenuatessteatosisandsuppressesinflammationindietinducednafldmice
AT zhouxiaoling tremellafuciformiscrudepolysaccharidesattenuatessteatosisandsuppressesinflammationindietinducednafldmice