Cargando…
Rapid Identification of Characteristic Chemical Constituents of Panax ginseng, Panax quinquefolius, and Panax japonicus Using UPLC-Q-TOF/MS
Saponins are the main active components in Panax ginseng C. A. Mey. (PG), Panax quinquefolius L. (PQ), and Panax japonicus C. A. Mey. (PJ), which belong to the genus Panax in the Araliaceae family. Because the chemical components in the three species are similar, they are often mixed and misused in...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8947929/ https://www.ncbi.nlm.nih.gov/pubmed/35340764 http://dx.doi.org/10.1155/2022/6463770 |
_version_ | 1784674553439453184 |
---|---|
author | Jinbiao, Liu Xinyue, Zhang Shenshen, Yang Shuo, Wang Chengcheng, Liu Bin, Yang Yubo, Li Ting, Cai |
author_facet | Jinbiao, Liu Xinyue, Zhang Shenshen, Yang Shuo, Wang Chengcheng, Liu Bin, Yang Yubo, Li Ting, Cai |
author_sort | Jinbiao, Liu |
collection | PubMed |
description | Saponins are the main active components in Panax ginseng C. A. Mey. (PG), Panax quinquefolius L. (PQ), and Panax japonicus C. A. Mey. (PJ), which belong to the genus Panax in the Araliaceae family. Because the chemical components in the three species are similar, they are often mixed and misused in functional foods and pharmaceuticals applications. Therefore, it is urgent to establish a method to quickly distinguish among PG, PQ, and PJ. Ultraperformance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was combined with data postprocessing to identify the main characteristic fragments (CFs) and the related neutral losses (NLs) of protopanaxadiol (PPD), protopanaxatriol (PPT), oleanolic acid (OLE), and ocotillol- (OCO-) type saponins. By comparing the mass spectral data, it was possible to rapidly classify and identify saponins in PG, PQ, and PJ. A total of twenty-three chemical components were identified in the PG samples, twenty-three components were identified in the PQ samples, and twenty-seven components were identified in the PJ samples. Among them, OCO-type saponins were characteristic of PQ and PJ. Ginsenoside Rf, which was absent from PQ, allowed for differentiation between PQ and PJ. The CFs and NLs in the mass spectra of the characteristic components of PG, PQ, and PJ allowed for the rapid classification and identification of these species. Additionally, these results provide technical support for the quality evaluation of Chinese herbal medicine and for constructing a scientific regulatory system. |
format | Online Article Text |
id | pubmed-8947929 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-89479292022-03-25 Rapid Identification of Characteristic Chemical Constituents of Panax ginseng, Panax quinquefolius, and Panax japonicus Using UPLC-Q-TOF/MS Jinbiao, Liu Xinyue, Zhang Shenshen, Yang Shuo, Wang Chengcheng, Liu Bin, Yang Yubo, Li Ting, Cai J Anal Methods Chem Research Article Saponins are the main active components in Panax ginseng C. A. Mey. (PG), Panax quinquefolius L. (PQ), and Panax japonicus C. A. Mey. (PJ), which belong to the genus Panax in the Araliaceae family. Because the chemical components in the three species are similar, they are often mixed and misused in functional foods and pharmaceuticals applications. Therefore, it is urgent to establish a method to quickly distinguish among PG, PQ, and PJ. Ultraperformance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was combined with data postprocessing to identify the main characteristic fragments (CFs) and the related neutral losses (NLs) of protopanaxadiol (PPD), protopanaxatriol (PPT), oleanolic acid (OLE), and ocotillol- (OCO-) type saponins. By comparing the mass spectral data, it was possible to rapidly classify and identify saponins in PG, PQ, and PJ. A total of twenty-three chemical components were identified in the PG samples, twenty-three components were identified in the PQ samples, and twenty-seven components were identified in the PJ samples. Among them, OCO-type saponins were characteristic of PQ and PJ. Ginsenoside Rf, which was absent from PQ, allowed for differentiation between PQ and PJ. The CFs and NLs in the mass spectra of the characteristic components of PG, PQ, and PJ allowed for the rapid classification and identification of these species. Additionally, these results provide technical support for the quality evaluation of Chinese herbal medicine and for constructing a scientific regulatory system. Hindawi 2022-02-15 /pmc/articles/PMC8947929/ /pubmed/35340764 http://dx.doi.org/10.1155/2022/6463770 Text en Copyright © 2022 Liu Jinbiao et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Jinbiao, Liu Xinyue, Zhang Shenshen, Yang Shuo, Wang Chengcheng, Liu Bin, Yang Yubo, Li Ting, Cai Rapid Identification of Characteristic Chemical Constituents of Panax ginseng, Panax quinquefolius, and Panax japonicus Using UPLC-Q-TOF/MS |
title | Rapid Identification of Characteristic Chemical Constituents of Panax ginseng, Panax quinquefolius, and Panax japonicus Using UPLC-Q-TOF/MS |
title_full | Rapid Identification of Characteristic Chemical Constituents of Panax ginseng, Panax quinquefolius, and Panax japonicus Using UPLC-Q-TOF/MS |
title_fullStr | Rapid Identification of Characteristic Chemical Constituents of Panax ginseng, Panax quinquefolius, and Panax japonicus Using UPLC-Q-TOF/MS |
title_full_unstemmed | Rapid Identification of Characteristic Chemical Constituents of Panax ginseng, Panax quinquefolius, and Panax japonicus Using UPLC-Q-TOF/MS |
title_short | Rapid Identification of Characteristic Chemical Constituents of Panax ginseng, Panax quinquefolius, and Panax japonicus Using UPLC-Q-TOF/MS |
title_sort | rapid identification of characteristic chemical constituents of panax ginseng, panax quinquefolius, and panax japonicus using uplc-q-tof/ms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8947929/ https://www.ncbi.nlm.nih.gov/pubmed/35340764 http://dx.doi.org/10.1155/2022/6463770 |
work_keys_str_mv | AT jinbiaoliu rapididentificationofcharacteristicchemicalconstituentsofpanaxginsengpanaxquinquefoliusandpanaxjaponicususinguplcqtofms AT xinyuezhang rapididentificationofcharacteristicchemicalconstituentsofpanaxginsengpanaxquinquefoliusandpanaxjaponicususinguplcqtofms AT shenshenyang rapididentificationofcharacteristicchemicalconstituentsofpanaxginsengpanaxquinquefoliusandpanaxjaponicususinguplcqtofms AT shuowang rapididentificationofcharacteristicchemicalconstituentsofpanaxginsengpanaxquinquefoliusandpanaxjaponicususinguplcqtofms AT chengchengliu rapididentificationofcharacteristicchemicalconstituentsofpanaxginsengpanaxquinquefoliusandpanaxjaponicususinguplcqtofms AT binyang rapididentificationofcharacteristicchemicalconstituentsofpanaxginsengpanaxquinquefoliusandpanaxjaponicususinguplcqtofms AT yuboli rapididentificationofcharacteristicchemicalconstituentsofpanaxginsengpanaxquinquefoliusandpanaxjaponicususinguplcqtofms AT tingcai rapididentificationofcharacteristicchemicalconstituentsofpanaxginsengpanaxquinquefoliusandpanaxjaponicususinguplcqtofms |