Synthesis of [7-(15)N]-GTPs for RNA structure and dynamics by NMR spectroscopy

Several isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-(15)N]-guanosine-5′-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcrip...

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Autores principales: Taiwo, Kehinde M., Olenginski, Lukasz T., Nußbaumer, Felix, Nam, Hyeyeon, Hilber, Stefan, Kreutz, Christoph, Dayie, T. Kwaku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2022
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948113/
https://www.ncbi.nlm.nih.gov/pubmed/35400760
http://dx.doi.org/10.1007/s00706-022-02892-1
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author Taiwo, Kehinde M.
Olenginski, Lukasz T.
Nußbaumer, Felix
Nam, Hyeyeon
Hilber, Stefan
Kreutz, Christoph
Dayie, T. Kwaku
author_facet Taiwo, Kehinde M.
Olenginski, Lukasz T.
Nußbaumer, Felix
Nam, Hyeyeon
Hilber, Stefan
Kreutz, Christoph
Dayie, T. Kwaku
author_sort Taiwo, Kehinde M.
collection PubMed
description Several isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-(15)N]-guanosine-5′-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcription. We showcase the utility of these labels to probe both structure and dynamics in two biologically important RNAs. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00706-022-02892-1.
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spelling pubmed-89481132022-04-07 Synthesis of [7-(15)N]-GTPs for RNA structure and dynamics by NMR spectroscopy Taiwo, Kehinde M. Olenginski, Lukasz T. Nußbaumer, Felix Nam, Hyeyeon Hilber, Stefan Kreutz, Christoph Dayie, T. Kwaku Monatsh Chem Original Paper Several isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-(15)N]-guanosine-5′-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcription. We showcase the utility of these labels to probe both structure and dynamics in two biologically important RNAs. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00706-022-02892-1. Springer Vienna 2022-02-26 2022 /pmc/articles/PMC8948113/ /pubmed/35400760 http://dx.doi.org/10.1007/s00706-022-02892-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Taiwo, Kehinde M.
Olenginski, Lukasz T.
Nußbaumer, Felix
Nam, Hyeyeon
Hilber, Stefan
Kreutz, Christoph
Dayie, T. Kwaku
Synthesis of [7-(15)N]-GTPs for RNA structure and dynamics by NMR spectroscopy
title Synthesis of [7-(15)N]-GTPs for RNA structure and dynamics by NMR spectroscopy
title_full Synthesis of [7-(15)N]-GTPs for RNA structure and dynamics by NMR spectroscopy
title_fullStr Synthesis of [7-(15)N]-GTPs for RNA structure and dynamics by NMR spectroscopy
title_full_unstemmed Synthesis of [7-(15)N]-GTPs for RNA structure and dynamics by NMR spectroscopy
title_short Synthesis of [7-(15)N]-GTPs for RNA structure and dynamics by NMR spectroscopy
title_sort synthesis of [7-(15)n]-gtps for rna structure and dynamics by nmr spectroscopy
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948113/
https://www.ncbi.nlm.nih.gov/pubmed/35400760
http://dx.doi.org/10.1007/s00706-022-02892-1
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