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Synthesis of [7-(15)N]-GTPs for RNA structure and dynamics by NMR spectroscopy

Several isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-(15)N]-guanosine-5′-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcrip...

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Detalles Bibliográficos
Autores principales:	Taiwo, Kehinde M., Olenginski, Lukasz T., Nußbaumer, Felix, Nam, Hyeyeon, Hilber, Stefan, Kreutz, Christoph, Dayie, T. Kwaku
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Vienna 2022
Materias:	Original Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948113/ https://www.ncbi.nlm.nih.gov/pubmed/35400760 http://dx.doi.org/10.1007/s00706-022-02892-1

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