Yeast-based production and in situ purification of acetaldehyde

Acetaldehyde is a platform chemical with a production volume of more than 1 Mt/a, but is chiefly synthesized from petrochemical feedstocks. We propose the fermentative conversion of glucose towards acetaldehyde via genetically modified S. cerevisiae. This allows for ethanol-free bioactaldehyde produ...

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Autores principales: Mengers, Hendrik G., Westarp, William Graf von, Brücker, Daniela, Jupke, Andreas, Blank, Lars M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948146/
https://www.ncbi.nlm.nih.gov/pubmed/35137261
http://dx.doi.org/10.1007/s00449-022-02697-w
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author Mengers, Hendrik G.
Westarp, William Graf von
Brücker, Daniela
Jupke, Andreas
Blank, Lars M.
author_facet Mengers, Hendrik G.
Westarp, William Graf von
Brücker, Daniela
Jupke, Andreas
Blank, Lars M.
author_sort Mengers, Hendrik G.
collection PubMed
description Acetaldehyde is a platform chemical with a production volume of more than 1 Mt/a, but is chiefly synthesized from petrochemical feedstocks. We propose the fermentative conversion of glucose towards acetaldehyde via genetically modified S. cerevisiae. This allows for ethanol-free bioactaldehyde production. Exploiting the high volatility of the product, in situ gas stripping in an aerated reactor is inevitable and crucial due to the respiratory toxicity effects of the acetaldehyde overproduction. We devise a lab-scale setup for the recovery of the product from the off-gas. Water was chosen as a suitable solvent and the Henry coefficient of acetaldehyde in water was validated experimentally. Based on an experimentally verified capture efficiency of 75%, an acetaldehyde production rate of over 100 mg/g/h was reached in 200 mL lab-scale fermentations. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00449-022-02697-w.
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spelling pubmed-89481462022-04-07 Yeast-based production and in situ purification of acetaldehyde Mengers, Hendrik G. Westarp, William Graf von Brücker, Daniela Jupke, Andreas Blank, Lars M. Bioprocess Biosyst Eng Research Paper Acetaldehyde is a platform chemical with a production volume of more than 1 Mt/a, but is chiefly synthesized from petrochemical feedstocks. We propose the fermentative conversion of glucose towards acetaldehyde via genetically modified S. cerevisiae. This allows for ethanol-free bioactaldehyde production. Exploiting the high volatility of the product, in situ gas stripping in an aerated reactor is inevitable and crucial due to the respiratory toxicity effects of the acetaldehyde overproduction. We devise a lab-scale setup for the recovery of the product from the off-gas. Water was chosen as a suitable solvent and the Henry coefficient of acetaldehyde in water was validated experimentally. Based on an experimentally verified capture efficiency of 75%, an acetaldehyde production rate of over 100 mg/g/h was reached in 200 mL lab-scale fermentations. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00449-022-02697-w. Springer Berlin Heidelberg 2022-02-08 2022 /pmc/articles/PMC8948146/ /pubmed/35137261 http://dx.doi.org/10.1007/s00449-022-02697-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Paper
Mengers, Hendrik G.
Westarp, William Graf von
Brücker, Daniela
Jupke, Andreas
Blank, Lars M.
Yeast-based production and in situ purification of acetaldehyde
title Yeast-based production and in situ purification of acetaldehyde
title_full Yeast-based production and in situ purification of acetaldehyde
title_fullStr Yeast-based production and in situ purification of acetaldehyde
title_full_unstemmed Yeast-based production and in situ purification of acetaldehyde
title_short Yeast-based production and in situ purification of acetaldehyde
title_sort yeast-based production and in situ purification of acetaldehyde
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948146/
https://www.ncbi.nlm.nih.gov/pubmed/35137261
http://dx.doi.org/10.1007/s00449-022-02697-w
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AT jupkeandreas yeastbasedproductionandinsitupurificationofacetaldehyde
AT blanklarsm yeastbasedproductionandinsitupurificationofacetaldehyde

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