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New approaches suggest term and preterm human fetal membranes may have distinct biomechanical properties

Preterm prelabour rupture of membranes is the leading cause of preterm birth and its associated infant mortality and morbidity. However, its underlying mechanism remains unknown. We utilized two novel biomechanical assessment techniques, ball indentation and Optical Coherence Elastography (OCE), to...

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Autores principales: Bhunia, Sudeshna, O’Brien, Shaughn, Ling, Yuting, Huang, Zhihong, Wu, Pensée, Yang, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948223/
https://www.ncbi.nlm.nih.gov/pubmed/35332209
http://dx.doi.org/10.1038/s41598-022-09005-2
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author Bhunia, Sudeshna
O’Brien, Shaughn
Ling, Yuting
Huang, Zhihong
Wu, Pensée
Yang, Ying
author_facet Bhunia, Sudeshna
O’Brien, Shaughn
Ling, Yuting
Huang, Zhihong
Wu, Pensée
Yang, Ying
author_sort Bhunia, Sudeshna
collection PubMed
description Preterm prelabour rupture of membranes is the leading cause of preterm birth and its associated infant mortality and morbidity. However, its underlying mechanism remains unknown. We utilized two novel biomechanical assessment techniques, ball indentation and Optical Coherence Elastography (OCE), to compare the mechanical properties and behaviours of term (≥ 37 weeks) and preterm (33–36 weeks) human fetal membranes from ruptured and non-ruptured regions. We defined the expression levels of collagen, sulfated glycosaminoglycans (sGAG), matrix metalloproteinase (MMP-9, MMP-13), fibronectin, and interleukin-1β (IL-1β) within membranes by biochemical analysis, immunohistochemical staining and Western blotting, both with and without simulated fetal movement forces on membrane rupture with a new loading system. Preterm membranes showed greater heterogeneity in mechanical properties/behaviours between ruptured and non-ruptured regions compared with their term counterparts (displacement rate: 36% vs. 15%; modulus: 125% vs. 34%; thickness: 93% vs. 30%; collagen content: 98% vs. 29%; sGAG: 85% vs 25%). Furthermore, simulated fetal movement forces triggered higher MMP-9, MMP-13 and IL-1β expression in preterm than term membranes, while nifedipine attenuated the observed increases in expression. In conclusion, the distinct biomechanical profiles of term and preterm membranes and the abnormal biochemical expression and activation by external forces in preterm membranes may provide insights into mechanisms of preterm rupture of membranes.
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spelling pubmed-89482232022-03-28 New approaches suggest term and preterm human fetal membranes may have distinct biomechanical properties Bhunia, Sudeshna O’Brien, Shaughn Ling, Yuting Huang, Zhihong Wu, Pensée Yang, Ying Sci Rep Article Preterm prelabour rupture of membranes is the leading cause of preterm birth and its associated infant mortality and morbidity. However, its underlying mechanism remains unknown. We utilized two novel biomechanical assessment techniques, ball indentation and Optical Coherence Elastography (OCE), to compare the mechanical properties and behaviours of term (≥ 37 weeks) and preterm (33–36 weeks) human fetal membranes from ruptured and non-ruptured regions. We defined the expression levels of collagen, sulfated glycosaminoglycans (sGAG), matrix metalloproteinase (MMP-9, MMP-13), fibronectin, and interleukin-1β (IL-1β) within membranes by biochemical analysis, immunohistochemical staining and Western blotting, both with and without simulated fetal movement forces on membrane rupture with a new loading system. Preterm membranes showed greater heterogeneity in mechanical properties/behaviours between ruptured and non-ruptured regions compared with their term counterparts (displacement rate: 36% vs. 15%; modulus: 125% vs. 34%; thickness: 93% vs. 30%; collagen content: 98% vs. 29%; sGAG: 85% vs 25%). Furthermore, simulated fetal movement forces triggered higher MMP-9, MMP-13 and IL-1β expression in preterm than term membranes, while nifedipine attenuated the observed increases in expression. In conclusion, the distinct biomechanical profiles of term and preterm membranes and the abnormal biochemical expression and activation by external forces in preterm membranes may provide insights into mechanisms of preterm rupture of membranes. Nature Publishing Group UK 2022-03-24 /pmc/articles/PMC8948223/ /pubmed/35332209 http://dx.doi.org/10.1038/s41598-022-09005-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Bhunia, Sudeshna
O’Brien, Shaughn
Ling, Yuting
Huang, Zhihong
Wu, Pensée
Yang, Ying
New approaches suggest term and preterm human fetal membranes may have distinct biomechanical properties
title New approaches suggest term and preterm human fetal membranes may have distinct biomechanical properties
title_full New approaches suggest term and preterm human fetal membranes may have distinct biomechanical properties
title_fullStr New approaches suggest term and preterm human fetal membranes may have distinct biomechanical properties
title_full_unstemmed New approaches suggest term and preterm human fetal membranes may have distinct biomechanical properties
title_short New approaches suggest term and preterm human fetal membranes may have distinct biomechanical properties
title_sort new approaches suggest term and preterm human fetal membranes may have distinct biomechanical properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948223/
https://www.ncbi.nlm.nih.gov/pubmed/35332209
http://dx.doi.org/10.1038/s41598-022-09005-2
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