Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing

Prime editing recently emerged as a next-generation approach for precise genome editing. Here we exploit DNA double-strand break (DSB) repair to develop two strategies that install precise genomic insertions using an SpCas9 nuclease-based prime editor (PEn). We first demonstrate that PEn coupled to...

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Detalles Bibliográficos
Autores principales: Peterka, Martin, Akrap, Nina, Li, Songyuan, Wimberger, Sandra, Hsieh, Pei-Pei, Degtev, Dmitrii, Bestas, Burcu, Barr, Jack, van de Plassche, Stijn, Mendoza-Garcia, Patricia, Šviković, Saša, Sienski, Grzegorz, Firth, Mike, Maresca, Marcello
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948305/
https://www.ncbi.nlm.nih.gov/pubmed/35332138
http://dx.doi.org/10.1038/s41467-022-28771-1

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