Multiphoton single-molecule localization by sequential excitation with light minima

Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1–2 nm precision with moderate photon counts, enabling tracking and super-resolution imaging with true molecular resolution. Expanding this concept to multi-pho...

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Detalles Bibliográficos
Autores principales: Masullo, Luciano A., Stefani, Fernando D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
News & Views
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948360/
https://www.ncbi.nlm.nih.gov/pubmed/35332123
http://dx.doi.org/10.1038/s41377-022-00763-2
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author Masullo, Luciano A.
Stefani, Fernando D.
author_facet Masullo, Luciano A.
Stefani, Fernando D.
author_sort Masullo, Luciano A.
collection PubMed
description Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1–2 nm precision with moderate photon counts, enabling tracking and super-resolution imaging with true molecular resolution. Expanding this concept to multi-photon regimes may be a useful complement to reach even higher localization precision and get deeper into biological specimens.
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spelling pubmed-89483602022-04-11 Multiphoton single-molecule localization by sequential excitation with light minima Masullo, Luciano A. Stefani, Fernando D. Light Sci Appl News & Views Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1–2 nm precision with moderate photon counts, enabling tracking and super-resolution imaging with true molecular resolution. Expanding this concept to multi-photon regimes may be a useful complement to reach even higher localization precision and get deeper into biological specimens. Nature Publishing Group UK 2022-03-25 /pmc/articles/PMC8948360/ /pubmed/35332123 http://dx.doi.org/10.1038/s41377-022-00763-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle News & Views
Masullo, Luciano A.
Stefani, Fernando D.
Multiphoton single-molecule localization by sequential excitation with light minima
title Multiphoton single-molecule localization by sequential excitation with light minima
title_full Multiphoton single-molecule localization by sequential excitation with light minima
title_fullStr Multiphoton single-molecule localization by sequential excitation with light minima
title_full_unstemmed Multiphoton single-molecule localization by sequential excitation with light minima
title_short Multiphoton single-molecule localization by sequential excitation with light minima
title_sort multiphoton single-molecule localization by sequential excitation with light minima
topic News & Views
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948360/
https://www.ncbi.nlm.nih.gov/pubmed/35332123
http://dx.doi.org/10.1038/s41377-022-00763-2
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