Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)

A novel neutral polysaccharide designated as PAP1b was isolated from Areca catechu L. by hot water extraction, ethanol precipitation, and column chromatography. PAP1b was mainly composed of mannose, galactose, xylose, and arabinose in a ratio of 4.1:3.3:0.9:1.7, with an average molecular weight of 3...

Descripción completa

Detalles Bibliográficos
Autores principales: Ji, Xiaolong, Guo, Jianhang, Pan, Feibing, Kuang, Fengjun, Chen, Haiming, Guo, Xudan, Liu, Yanqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Nutrition
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948432/
https://www.ncbi.nlm.nih.gov/pubmed/35340550
http://dx.doi.org/10.3389/fnut.2022.853115
_version_ 1784674657884962816
author Ji, Xiaolong
Guo, Jianhang
Pan, Feibing
Kuang, Fengjun
Chen, Haiming
Guo, Xudan
Liu, Yanqi
author_facet Ji, Xiaolong
Guo, Jianhang
Pan, Feibing
Kuang, Fengjun
Chen, Haiming
Guo, Xudan
Liu, Yanqi
author_sort Ji, Xiaolong
collection PubMed
description A novel neutral polysaccharide designated as PAP1b was isolated from Areca catechu L. by hot water extraction, ethanol precipitation, and column chromatography. PAP1b was mainly composed of mannose, galactose, xylose, and arabinose in a ratio of 4.1:3.3:0.9:1.7, with an average molecular weight of 37.3 kDa. Structural characterization indicated that the backbone of PAP1b appeared to be composed mainly of → 6-β-Manp-(1 →, → 4)-α-Galp-(1 → and → 3,6)-β-Manp-(1 →) residues with some branches, and terminal of (1 →)-linked-β-Manp residues. The results of bioactivity experiments showed that PAP1b had antioxidant in vitro, esspecially on scavenging DPPH and hydroxyl radicals. Therefore, the polysaccharide from Areca catechu L. could be used as a potential antioxidant in functional food.
format Online
Article
Text
id pubmed-8948432
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-89484322022-03-26 Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.) Ji, Xiaolong Guo, Jianhang Pan, Feibing Kuang, Fengjun Chen, Haiming Guo, Xudan Liu, Yanqi Front Nutr Nutrition A novel neutral polysaccharide designated as PAP1b was isolated from Areca catechu L. by hot water extraction, ethanol precipitation, and column chromatography. PAP1b was mainly composed of mannose, galactose, xylose, and arabinose in a ratio of 4.1:3.3:0.9:1.7, with an average molecular weight of 37.3 kDa. Structural characterization indicated that the backbone of PAP1b appeared to be composed mainly of → 6-β-Manp-(1 →, → 4)-α-Galp-(1 → and → 3,6)-β-Manp-(1 →) residues with some branches, and terminal of (1 →)-linked-β-Manp residues. The results of bioactivity experiments showed that PAP1b had antioxidant in vitro, esspecially on scavenging DPPH and hydroxyl radicals. Therefore, the polysaccharide from Areca catechu L. could be used as a potential antioxidant in functional food. Frontiers Media S.A. 2022-03-07 /pmc/articles/PMC8948432/ /pubmed/35340550 http://dx.doi.org/10.3389/fnut.2022.853115 Text en Copyright © 2022 Ji, Guo, Pan, Kuang, Chen, Guo and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Ji, Xiaolong
Guo, Jianhang
Pan, Feibing
Kuang, Fengjun
Chen, Haiming
Guo, Xudan
Liu, Yanqi
Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)
title Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)
title_full Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)
title_fullStr Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)
title_full_unstemmed Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)
title_short Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)
title_sort structural elucidation and antioxidant activities of a neutral polysaccharide from arecanut (areca catechu l.)
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948432/
https://www.ncbi.nlm.nih.gov/pubmed/35340550
http://dx.doi.org/10.3389/fnut.2022.853115
work_keys_str_mv AT jixiaolong structuralelucidationandantioxidantactivitiesofaneutralpolysaccharidefromarecanutarecacatechul
AT guojianhang structuralelucidationandantioxidantactivitiesofaneutralpolysaccharidefromarecanutarecacatechul
AT panfeibing structuralelucidationandantioxidantactivitiesofaneutralpolysaccharidefromarecanutarecacatechul
AT kuangfengjun structuralelucidationandantioxidantactivitiesofaneutralpolysaccharidefromarecanutarecacatechul
AT chenhaiming structuralelucidationandantioxidantactivitiesofaneutralpolysaccharidefromarecanutarecacatechul
AT guoxudan structuralelucidationandantioxidantactivitiesofaneutralpolysaccharidefromarecanutarecacatechul
AT liuyanqi structuralelucidationandantioxidantactivitiesofaneutralpolysaccharidefromarecanutarecacatechul

Ejemplares similares