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Fish Capsules: A System for High‐Throughput Screening of Combinatorial Drugs
Large‐scale screening of molecules heavily relies on phenotyping of small living organisms during preclinical development. However, deep profiling candidate therapeutics on whole animals typically requires laborious manipulations and anesthetic treatment using traditional techniques or automated too...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948576/ https://www.ncbi.nlm.nih.gov/pubmed/35088577 http://dx.doi.org/10.1002/advs.202104449 |
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author | Tang, Minghui Duan, Xin Yang, Anqi He, Shijie Zhou, Yajing Liu, Yuxin Zhang, Lu Luo, Xuan Shi, Peng Li, Honglin Lin, Xudong |
author_facet | Tang, Minghui Duan, Xin Yang, Anqi He, Shijie Zhou, Yajing Liu, Yuxin Zhang, Lu Luo, Xuan Shi, Peng Li, Honglin Lin, Xudong |
author_sort | Tang, Minghui |
collection | PubMed |
description | Large‐scale screening of molecules heavily relies on phenotyping of small living organisms during preclinical development. However, deep profiling candidate therapeutics on whole animals typically requires laborious manipulations and anesthetic treatment using traditional techniques or automated tools. Here, a novel fish capsule system that combines automated zebrafish encapsulating technology and droplet microarray strategy for in vivo functional screening of mono/polytherapies is described. This platform enables automated, rapid zebrafish orientation and immobilization in agarose to generate large‐scale fish capsules by using a microfluidic device. Based on the effect of discontinuous dewetting, the prompt trapping of fish capsules in the aqueous arrays is successfully demonstrate. This system provides the capability to integrate pharmaceutical treatments with real‐time multispectral microscopic imaging in a simple, pipetting‐free and highly parallel manner. Coupling with machine learning algorithms, a small library of compounds is screened and analyzed, and clues about how to exploit compound combinations as therapeutic candidates are obtained. It is believed that this proposed strategy can be readily applied to multiple fields and is especially useful in the exploration of combinatorial drugs with limited amounts of samples and resources to accelerate the identification of novel therapeutics for precision medicines. |
format | Online Article Text |
id | pubmed-8948576 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89485762022-03-29 Fish Capsules: A System for High‐Throughput Screening of Combinatorial Drugs Tang, Minghui Duan, Xin Yang, Anqi He, Shijie Zhou, Yajing Liu, Yuxin Zhang, Lu Luo, Xuan Shi, Peng Li, Honglin Lin, Xudong Adv Sci (Weinh) Research Articles Large‐scale screening of molecules heavily relies on phenotyping of small living organisms during preclinical development. However, deep profiling candidate therapeutics on whole animals typically requires laborious manipulations and anesthetic treatment using traditional techniques or automated tools. Here, a novel fish capsule system that combines automated zebrafish encapsulating technology and droplet microarray strategy for in vivo functional screening of mono/polytherapies is described. This platform enables automated, rapid zebrafish orientation and immobilization in agarose to generate large‐scale fish capsules by using a microfluidic device. Based on the effect of discontinuous dewetting, the prompt trapping of fish capsules in the aqueous arrays is successfully demonstrate. This system provides the capability to integrate pharmaceutical treatments with real‐time multispectral microscopic imaging in a simple, pipetting‐free and highly parallel manner. Coupling with machine learning algorithms, a small library of compounds is screened and analyzed, and clues about how to exploit compound combinations as therapeutic candidates are obtained. It is believed that this proposed strategy can be readily applied to multiple fields and is especially useful in the exploration of combinatorial drugs with limited amounts of samples and resources to accelerate the identification of novel therapeutics for precision medicines. John Wiley and Sons Inc. 2022-01-27 /pmc/articles/PMC8948576/ /pubmed/35088577 http://dx.doi.org/10.1002/advs.202104449 Text en © 2022 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Tang, Minghui Duan, Xin Yang, Anqi He, Shijie Zhou, Yajing Liu, Yuxin Zhang, Lu Luo, Xuan Shi, Peng Li, Honglin Lin, Xudong Fish Capsules: A System for High‐Throughput Screening of Combinatorial Drugs |
title | Fish Capsules: A System for High‐Throughput Screening of Combinatorial Drugs |
title_full | Fish Capsules: A System for High‐Throughput Screening of Combinatorial Drugs |
title_fullStr | Fish Capsules: A System for High‐Throughput Screening of Combinatorial Drugs |
title_full_unstemmed | Fish Capsules: A System for High‐Throughput Screening of Combinatorial Drugs |
title_short | Fish Capsules: A System for High‐Throughput Screening of Combinatorial Drugs |
title_sort | fish capsules: a system for high‐throughput screening of combinatorial drugs |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948576/ https://www.ncbi.nlm.nih.gov/pubmed/35088577 http://dx.doi.org/10.1002/advs.202104449 |
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