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Transfer of Human Microbiome to Drosophila Gut Model
Laboratory animals with human microbiome have increasingly been used to study the role of bacteria and host interaction. Drosophila melanogaster, as a model of microbiota-host interaction with high reproductive efficiency and high availability, has always been lacking studies of interaction with hum...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948740/ https://www.ncbi.nlm.nih.gov/pubmed/35336128 http://dx.doi.org/10.3390/microorganisms10030553 |
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author | Ji, Dongxu Sun, Hao Yang, Weichao Gao, Mingfu Xu, Hui |
author_facet | Ji, Dongxu Sun, Hao Yang, Weichao Gao, Mingfu Xu, Hui |
author_sort | Ji, Dongxu |
collection | PubMed |
description | Laboratory animals with human microbiome have increasingly been used to study the role of bacteria and host interaction. Drosophila melanogaster, as a model of microbiota-host interaction with high reproductive efficiency and high availability, has always been lacking studies of interaction with human gut microbiome. In this study, we attempted to use antibiotic therapy and human fecal exposure strategy to transfer the human microbiome to the drosophila. The method includes depleting the original intestinal bacteria using a broad-spectrum antibiotic and then introducing human microorganisms by a diet supplemented with donor’s fecal samples. The sequencing results showed that 80–87.5% of the OTUs (Operational Taxonomic Units) from donor feces were adopted by the recipient drosophila following 30 days of observation. In comparison to females, the male recipient drosophila inherited more microbiota from the donor feces and had significantly increased lifespan as well as improved vertical climbing ability. Furthermore, distinctly differential expression patterns for age and insulin-like signaling-related genes were obtained for the male vs. female recipients. Only the male drosophila offspring acquired the characteristics of the donor fecal microbiota. |
format | Online Article Text |
id | pubmed-8948740 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89487402022-03-26 Transfer of Human Microbiome to Drosophila Gut Model Ji, Dongxu Sun, Hao Yang, Weichao Gao, Mingfu Xu, Hui Microorganisms Article Laboratory animals with human microbiome have increasingly been used to study the role of bacteria and host interaction. Drosophila melanogaster, as a model of microbiota-host interaction with high reproductive efficiency and high availability, has always been lacking studies of interaction with human gut microbiome. In this study, we attempted to use antibiotic therapy and human fecal exposure strategy to transfer the human microbiome to the drosophila. The method includes depleting the original intestinal bacteria using a broad-spectrum antibiotic and then introducing human microorganisms by a diet supplemented with donor’s fecal samples. The sequencing results showed that 80–87.5% of the OTUs (Operational Taxonomic Units) from donor feces were adopted by the recipient drosophila following 30 days of observation. In comparison to females, the male recipient drosophila inherited more microbiota from the donor feces and had significantly increased lifespan as well as improved vertical climbing ability. Furthermore, distinctly differential expression patterns for age and insulin-like signaling-related genes were obtained for the male vs. female recipients. Only the male drosophila offspring acquired the characteristics of the donor fecal microbiota. MDPI 2022-03-03 /pmc/articles/PMC8948740/ /pubmed/35336128 http://dx.doi.org/10.3390/microorganisms10030553 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ji, Dongxu Sun, Hao Yang, Weichao Gao, Mingfu Xu, Hui Transfer of Human Microbiome to Drosophila Gut Model |
title | Transfer of Human Microbiome to Drosophila Gut Model |
title_full | Transfer of Human Microbiome to Drosophila Gut Model |
title_fullStr | Transfer of Human Microbiome to Drosophila Gut Model |
title_full_unstemmed | Transfer of Human Microbiome to Drosophila Gut Model |
title_short | Transfer of Human Microbiome to Drosophila Gut Model |
title_sort | transfer of human microbiome to drosophila gut model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948740/ https://www.ncbi.nlm.nih.gov/pubmed/35336128 http://dx.doi.org/10.3390/microorganisms10030553 |
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