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Transfer of Human Microbiome to Drosophila Gut Model

Laboratory animals with human microbiome have increasingly been used to study the role of bacteria and host interaction. Drosophila melanogaster, as a model of microbiota-host interaction with high reproductive efficiency and high availability, has always been lacking studies of interaction with hum...

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Detalles Bibliográficos
Autores principales: Ji, Dongxu, Sun, Hao, Yang, Weichao, Gao, Mingfu, Xu, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948740/
https://www.ncbi.nlm.nih.gov/pubmed/35336128
http://dx.doi.org/10.3390/microorganisms10030553
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author Ji, Dongxu
Sun, Hao
Yang, Weichao
Gao, Mingfu
Xu, Hui
author_facet Ji, Dongxu
Sun, Hao
Yang, Weichao
Gao, Mingfu
Xu, Hui
author_sort Ji, Dongxu
collection PubMed
description Laboratory animals with human microbiome have increasingly been used to study the role of bacteria and host interaction. Drosophila melanogaster, as a model of microbiota-host interaction with high reproductive efficiency and high availability, has always been lacking studies of interaction with human gut microbiome. In this study, we attempted to use antibiotic therapy and human fecal exposure strategy to transfer the human microbiome to the drosophila. The method includes depleting the original intestinal bacteria using a broad-spectrum antibiotic and then introducing human microorganisms by a diet supplemented with donor’s fecal samples. The sequencing results showed that 80–87.5% of the OTUs (Operational Taxonomic Units) from donor feces were adopted by the recipient drosophila following 30 days of observation. In comparison to females, the male recipient drosophila inherited more microbiota from the donor feces and had significantly increased lifespan as well as improved vertical climbing ability. Furthermore, distinctly differential expression patterns for age and insulin-like signaling-related genes were obtained for the male vs. female recipients. Only the male drosophila offspring acquired the characteristics of the donor fecal microbiota.
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spelling pubmed-89487402022-03-26 Transfer of Human Microbiome to Drosophila Gut Model Ji, Dongxu Sun, Hao Yang, Weichao Gao, Mingfu Xu, Hui Microorganisms Article Laboratory animals with human microbiome have increasingly been used to study the role of bacteria and host interaction. Drosophila melanogaster, as a model of microbiota-host interaction with high reproductive efficiency and high availability, has always been lacking studies of interaction with human gut microbiome. In this study, we attempted to use antibiotic therapy and human fecal exposure strategy to transfer the human microbiome to the drosophila. The method includes depleting the original intestinal bacteria using a broad-spectrum antibiotic and then introducing human microorganisms by a diet supplemented with donor’s fecal samples. The sequencing results showed that 80–87.5% of the OTUs (Operational Taxonomic Units) from donor feces were adopted by the recipient drosophila following 30 days of observation. In comparison to females, the male recipient drosophila inherited more microbiota from the donor feces and had significantly increased lifespan as well as improved vertical climbing ability. Furthermore, distinctly differential expression patterns for age and insulin-like signaling-related genes were obtained for the male vs. female recipients. Only the male drosophila offspring acquired the characteristics of the donor fecal microbiota. MDPI 2022-03-03 /pmc/articles/PMC8948740/ /pubmed/35336128 http://dx.doi.org/10.3390/microorganisms10030553 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ji, Dongxu
Sun, Hao
Yang, Weichao
Gao, Mingfu
Xu, Hui
Transfer of Human Microbiome to Drosophila Gut Model
title Transfer of Human Microbiome to Drosophila Gut Model
title_full Transfer of Human Microbiome to Drosophila Gut Model
title_fullStr Transfer of Human Microbiome to Drosophila Gut Model
title_full_unstemmed Transfer of Human Microbiome to Drosophila Gut Model
title_short Transfer of Human Microbiome to Drosophila Gut Model
title_sort transfer of human microbiome to drosophila gut model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8948740/
https://www.ncbi.nlm.nih.gov/pubmed/35336128
http://dx.doi.org/10.3390/microorganisms10030553
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