Effect of Salinity on Growth, Ion Accumulation and Mineral Nutrition of Different Accessions of a Crop Wild Relative Legume Species, Trifolium fragiferum

Crop wild relatives represent a valuable resource for the breeding of new crop varieties suitable for sustainable productivity in conditions of climate change. The aim of the present study was to assess salt tolerance of several wild accessions of T. fragiferum from habitats with different salinity levels in controlled conditions. Decrease of plant biomass and changes in partitioning between different organs was a characteristic response of plants with increasing substrate salinity, but these responses were genotype-specific. In several accessions, salinity stimulated reproductive development. The major differences in salinity responses between various T. fragiferum genotypes were at the level of dry biomass accumulation as well as water accumulation in plant tissues, resulting in relatively more similar effect on fresh mass. Na(+) and Cl(−) accumulation capacity were organ-specific, with leaf petioles accumulating more, followed by leaf blades and stolons. Responses of mineral nutrition clearly were both genotype- and organ-specific, but several elements showed a relatively general pattern, such as increase in Zn concentration in all plant parts, and decrease in Ca and Mg concentration. Alterations in mineralome possibly reflect a reprogramming of the metabolism to adapt to changes in growth, morphology and ion accumulation resulting from effect of NaCl. High intraspecies morphological and physiological variability in responses of T. fragiferum accessions to salinity allow to describe them as ecotypes.