Cargando…

Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart

In recent years the blood proteome has been increasingly researched for biomarkers for early detection of colorectal cancer (CRC). Blood samples from screening studies are often subject to preanalytical variability and repeated freeze–thaw cycles. We aimed to assess the correlation of repeat measure...

Descripción completa

Detalles Bibliográficos
Autores principales: Bhardwaj, Megha, Schrotz-King, Petra, Brenner, Hermann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949042/
https://www.ncbi.nlm.nih.gov/pubmed/35330110
http://dx.doi.org/10.3390/life12030359
_version_ 1784674799749955584
author Bhardwaj, Megha
Schrotz-King, Petra
Brenner, Hermann
author_facet Bhardwaj, Megha
Schrotz-King, Petra
Brenner, Hermann
author_sort Bhardwaj, Megha
collection PubMed
description In recent years the blood proteome has been increasingly researched for biomarkers for early detection of colorectal cancer (CRC). Blood samples from screening studies are often subject to preanalytical variability and repeated freeze–thaw cycles. We aimed to assess the correlation of repeat measurements of 27 candidate protein markers for CRC screening taken three years and multiple freeze–thaw cycles apart. The concentrations of 27 protein markers were measured in plasma samples of 39 newly detected CRC cases from a cohort of 9245 participants of screening colonoscopies. The proteins were measured using proximity extension assays (PEA) carried out on the same set of samples twice, three years apart, with an average of three freeze–thaw cycles in between the two measurements. Pearson’s product moment correlation coefficients were calculated. Correlation coefficients ranged from +0.43 to +0.97, with a median of 0.67 and an interquartile range of +0.58 to +0.84, with all p-values of correlation being <0.01 (<0.0005 for 22 proteins, <0.001 for 4 proteins). Repeat measurements of the 27 protein biomarkers for CRC screening performed three years later, and on average three freeze–thaw cycles apart, showed moderate to high levels of correlation. Apart from the effects of freeze–thaw cycles, slightly different preprocessing performed on the data may have contributed to recorded differences between measurements.
format Online
Article
Text
id pubmed-8949042
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89490422022-03-26 Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart Bhardwaj, Megha Schrotz-King, Petra Brenner, Hermann Life (Basel) Article In recent years the blood proteome has been increasingly researched for biomarkers for early detection of colorectal cancer (CRC). Blood samples from screening studies are often subject to preanalytical variability and repeated freeze–thaw cycles. We aimed to assess the correlation of repeat measurements of 27 candidate protein markers for CRC screening taken three years and multiple freeze–thaw cycles apart. The concentrations of 27 protein markers were measured in plasma samples of 39 newly detected CRC cases from a cohort of 9245 participants of screening colonoscopies. The proteins were measured using proximity extension assays (PEA) carried out on the same set of samples twice, three years apart, with an average of three freeze–thaw cycles in between the two measurements. Pearson’s product moment correlation coefficients were calculated. Correlation coefficients ranged from +0.43 to +0.97, with a median of 0.67 and an interquartile range of +0.58 to +0.84, with all p-values of correlation being <0.01 (<0.0005 for 22 proteins, <0.001 for 4 proteins). Repeat measurements of the 27 protein biomarkers for CRC screening performed three years later, and on average three freeze–thaw cycles apart, showed moderate to high levels of correlation. Apart from the effects of freeze–thaw cycles, slightly different preprocessing performed on the data may have contributed to recorded differences between measurements. MDPI 2022-03-02 /pmc/articles/PMC8949042/ /pubmed/35330110 http://dx.doi.org/10.3390/life12030359 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bhardwaj, Megha
Schrotz-King, Petra
Brenner, Hermann
Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart
title Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart
title_full Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart
title_fullStr Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart
title_full_unstemmed Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart
title_short Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart
title_sort correlation of repeat measurements of 27 candidate protein markers for colorectal cancer screening taken three years and multiple freeze–thaw cycles apart
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949042/
https://www.ncbi.nlm.nih.gov/pubmed/35330110
http://dx.doi.org/10.3390/life12030359
work_keys_str_mv AT bhardwajmegha correlationofrepeatmeasurementsof27candidateproteinmarkersforcolorectalcancerscreeningtakenthreeyearsandmultiplefreezethawcyclesapart
AT schrotzkingpetra correlationofrepeatmeasurementsof27candidateproteinmarkersforcolorectalcancerscreeningtakenthreeyearsandmultiplefreezethawcyclesapart
AT brennerhermann correlationofrepeatmeasurementsof27candidateproteinmarkersforcolorectalcancerscreeningtakenthreeyearsandmultiplefreezethawcyclesapart