Cargando…
Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart
In recent years the blood proteome has been increasingly researched for biomarkers for early detection of colorectal cancer (CRC). Blood samples from screening studies are often subject to preanalytical variability and repeated freeze–thaw cycles. We aimed to assess the correlation of repeat measure...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949042/ https://www.ncbi.nlm.nih.gov/pubmed/35330110 http://dx.doi.org/10.3390/life12030359 |
_version_ | 1784674799749955584 |
---|---|
author | Bhardwaj, Megha Schrotz-King, Petra Brenner, Hermann |
author_facet | Bhardwaj, Megha Schrotz-King, Petra Brenner, Hermann |
author_sort | Bhardwaj, Megha |
collection | PubMed |
description | In recent years the blood proteome has been increasingly researched for biomarkers for early detection of colorectal cancer (CRC). Blood samples from screening studies are often subject to preanalytical variability and repeated freeze–thaw cycles. We aimed to assess the correlation of repeat measurements of 27 candidate protein markers for CRC screening taken three years and multiple freeze–thaw cycles apart. The concentrations of 27 protein markers were measured in plasma samples of 39 newly detected CRC cases from a cohort of 9245 participants of screening colonoscopies. The proteins were measured using proximity extension assays (PEA) carried out on the same set of samples twice, three years apart, with an average of three freeze–thaw cycles in between the two measurements. Pearson’s product moment correlation coefficients were calculated. Correlation coefficients ranged from +0.43 to +0.97, with a median of 0.67 and an interquartile range of +0.58 to +0.84, with all p-values of correlation being <0.01 (<0.0005 for 22 proteins, <0.001 for 4 proteins). Repeat measurements of the 27 protein biomarkers for CRC screening performed three years later, and on average three freeze–thaw cycles apart, showed moderate to high levels of correlation. Apart from the effects of freeze–thaw cycles, slightly different preprocessing performed on the data may have contributed to recorded differences between measurements. |
format | Online Article Text |
id | pubmed-8949042 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89490422022-03-26 Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart Bhardwaj, Megha Schrotz-King, Petra Brenner, Hermann Life (Basel) Article In recent years the blood proteome has been increasingly researched for biomarkers for early detection of colorectal cancer (CRC). Blood samples from screening studies are often subject to preanalytical variability and repeated freeze–thaw cycles. We aimed to assess the correlation of repeat measurements of 27 candidate protein markers for CRC screening taken three years and multiple freeze–thaw cycles apart. The concentrations of 27 protein markers were measured in plasma samples of 39 newly detected CRC cases from a cohort of 9245 participants of screening colonoscopies. The proteins were measured using proximity extension assays (PEA) carried out on the same set of samples twice, three years apart, with an average of three freeze–thaw cycles in between the two measurements. Pearson’s product moment correlation coefficients were calculated. Correlation coefficients ranged from +0.43 to +0.97, with a median of 0.67 and an interquartile range of +0.58 to +0.84, with all p-values of correlation being <0.01 (<0.0005 for 22 proteins, <0.001 for 4 proteins). Repeat measurements of the 27 protein biomarkers for CRC screening performed three years later, and on average three freeze–thaw cycles apart, showed moderate to high levels of correlation. Apart from the effects of freeze–thaw cycles, slightly different preprocessing performed on the data may have contributed to recorded differences between measurements. MDPI 2022-03-02 /pmc/articles/PMC8949042/ /pubmed/35330110 http://dx.doi.org/10.3390/life12030359 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bhardwaj, Megha Schrotz-King, Petra Brenner, Hermann Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart |
title | Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart |
title_full | Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart |
title_fullStr | Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart |
title_full_unstemmed | Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart |
title_short | Correlation of Repeat Measurements of 27 Candidate Protein Markers for Colorectal Cancer Screening Taken Three Years and Multiple Freeze–Thaw Cycles Apart |
title_sort | correlation of repeat measurements of 27 candidate protein markers for colorectal cancer screening taken three years and multiple freeze–thaw cycles apart |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949042/ https://www.ncbi.nlm.nih.gov/pubmed/35330110 http://dx.doi.org/10.3390/life12030359 |
work_keys_str_mv | AT bhardwajmegha correlationofrepeatmeasurementsof27candidateproteinmarkersforcolorectalcancerscreeningtakenthreeyearsandmultiplefreezethawcyclesapart AT schrotzkingpetra correlationofrepeatmeasurementsof27candidateproteinmarkersforcolorectalcancerscreeningtakenthreeyearsandmultiplefreezethawcyclesapart AT brennerhermann correlationofrepeatmeasurementsof27candidateproteinmarkersforcolorectalcancerscreeningtakenthreeyearsandmultiplefreezethawcyclesapart |