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Metabolomic and Physiological Changes in Fagus sylvatica Seedlings Infected with Phytophthora plurivora and the A1 and A2 Mating Types of P. ×cambivora

Phytophthora infections are followed by histological alterations, physiological and metabolomic adjustments in the host but very few studies contemplate these changes simultaneously. Fagus sylvatica seedlings were inoculated with A1 and A2 mating types of the heterothallic P. ×cambivora and with the...

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Autores principales: Corcobado, Tamara, Milenković, Ivan, Saiz-Fernández, Iñigo, Kudláček, Tomáš, Plichta, Roman, Májek, Tomáš, Bačová, Aneta, Ďatková, Henrieta, Dálya, László Benedek, Trifković, Miloš, Mureddu, Davide, Račko, Vladimír, Kardošová, Monika, Ďurkovič, Jaroslav, Rattunde, Roman, Jung, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949215/
https://www.ncbi.nlm.nih.gov/pubmed/35330301
http://dx.doi.org/10.3390/jof8030298
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author Corcobado, Tamara
Milenković, Ivan
Saiz-Fernández, Iñigo
Kudláček, Tomáš
Plichta, Roman
Májek, Tomáš
Bačová, Aneta
Ďatková, Henrieta
Dálya, László Benedek
Trifković, Miloš
Mureddu, Davide
Račko, Vladimír
Kardošová, Monika
Ďurkovič, Jaroslav
Rattunde, Roman
Jung, Thomas
author_facet Corcobado, Tamara
Milenković, Ivan
Saiz-Fernández, Iñigo
Kudláček, Tomáš
Plichta, Roman
Májek, Tomáš
Bačová, Aneta
Ďatková, Henrieta
Dálya, László Benedek
Trifković, Miloš
Mureddu, Davide
Račko, Vladimír
Kardošová, Monika
Ďurkovič, Jaroslav
Rattunde, Roman
Jung, Thomas
author_sort Corcobado, Tamara
collection PubMed
description Phytophthora infections are followed by histological alterations, physiological and metabolomic adjustments in the host but very few studies contemplate these changes simultaneously. Fagus sylvatica seedlings were inoculated with A1 and A2 mating types of the heterothallic P. ×cambivora and with the homothallic P. plurivora to identify plant physiological and metabolomic changes accompanying microscope observations of the colonization process one, two and three weeks after inoculation. Phytophthora plurivora-infected plants died at a faster pace than those inoculated with P. ×cambivora and showed higher mortality than P. ×cambivora A1-infected plants. Phytophthora ×cambivora A1 and A2 caused similar progression and total rate of mortality. Most differences in the physiological parameters between inoculated and non-inoculated plants were detected two weeks after inoculation. Alterations in primary and secondary metabolites in roots and leaves were demonstrated for all the inoculated plants two and three weeks after inoculation. The results indicate that P. plurivora is more aggressive to Fagus sylvatica seedlings than both mating types of P. ×cambivora while P. ×cambivora A1 showed a slower infection mode than P. ×cambivora A2 and led to minor plant metabolomic adjustments.
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spelling pubmed-89492152022-03-26 Metabolomic and Physiological Changes in Fagus sylvatica Seedlings Infected with Phytophthora plurivora and the A1 and A2 Mating Types of P. ×cambivora Corcobado, Tamara Milenković, Ivan Saiz-Fernández, Iñigo Kudláček, Tomáš Plichta, Roman Májek, Tomáš Bačová, Aneta Ďatková, Henrieta Dálya, László Benedek Trifković, Miloš Mureddu, Davide Račko, Vladimír Kardošová, Monika Ďurkovič, Jaroslav Rattunde, Roman Jung, Thomas J Fungi (Basel) Article Phytophthora infections are followed by histological alterations, physiological and metabolomic adjustments in the host but very few studies contemplate these changes simultaneously. Fagus sylvatica seedlings were inoculated with A1 and A2 mating types of the heterothallic P. ×cambivora and with the homothallic P. plurivora to identify plant physiological and metabolomic changes accompanying microscope observations of the colonization process one, two and three weeks after inoculation. Phytophthora plurivora-infected plants died at a faster pace than those inoculated with P. ×cambivora and showed higher mortality than P. ×cambivora A1-infected plants. Phytophthora ×cambivora A1 and A2 caused similar progression and total rate of mortality. Most differences in the physiological parameters between inoculated and non-inoculated plants were detected two weeks after inoculation. Alterations in primary and secondary metabolites in roots and leaves were demonstrated for all the inoculated plants two and three weeks after inoculation. The results indicate that P. plurivora is more aggressive to Fagus sylvatica seedlings than both mating types of P. ×cambivora while P. ×cambivora A1 showed a slower infection mode than P. ×cambivora A2 and led to minor plant metabolomic adjustments. MDPI 2022-03-14 /pmc/articles/PMC8949215/ /pubmed/35330301 http://dx.doi.org/10.3390/jof8030298 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Corcobado, Tamara
Milenković, Ivan
Saiz-Fernández, Iñigo
Kudláček, Tomáš
Plichta, Roman
Májek, Tomáš
Bačová, Aneta
Ďatková, Henrieta
Dálya, László Benedek
Trifković, Miloš
Mureddu, Davide
Račko, Vladimír
Kardošová, Monika
Ďurkovič, Jaroslav
Rattunde, Roman
Jung, Thomas
Metabolomic and Physiological Changes in Fagus sylvatica Seedlings Infected with Phytophthora plurivora and the A1 and A2 Mating Types of P. ×cambivora
title Metabolomic and Physiological Changes in Fagus sylvatica Seedlings Infected with Phytophthora plurivora and the A1 and A2 Mating Types of P. ×cambivora
title_full Metabolomic and Physiological Changes in Fagus sylvatica Seedlings Infected with Phytophthora plurivora and the A1 and A2 Mating Types of P. ×cambivora
title_fullStr Metabolomic and Physiological Changes in Fagus sylvatica Seedlings Infected with Phytophthora plurivora and the A1 and A2 Mating Types of P. ×cambivora
title_full_unstemmed Metabolomic and Physiological Changes in Fagus sylvatica Seedlings Infected with Phytophthora plurivora and the A1 and A2 Mating Types of P. ×cambivora
title_short Metabolomic and Physiological Changes in Fagus sylvatica Seedlings Infected with Phytophthora plurivora and the A1 and A2 Mating Types of P. ×cambivora
title_sort metabolomic and physiological changes in fagus sylvatica seedlings infected with phytophthora plurivora and the a1 and a2 mating types of p. ×cambivora
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949215/
https://www.ncbi.nlm.nih.gov/pubmed/35330301
http://dx.doi.org/10.3390/jof8030298
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