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A Novel H-2(d) Epitope for Influenza A Polymerase Acidic Protein
Understanding the complexity of the T-cell epitope hierarchy in humans through mouse models can be difficult. In particular, using only one murine strain, the C57BL/6 mouse, to investigate the immune response to influenza virus infection limits our understanding. In the present study, by immunizing...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949235/ https://www.ncbi.nlm.nih.gov/pubmed/35337006 http://dx.doi.org/10.3390/v14030601 |
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author | Uddbäck, Ida Kohlmeier, Jacob E. Thomsen, Allan R. Christensen, Jan P. |
author_facet | Uddbäck, Ida Kohlmeier, Jacob E. Thomsen, Allan R. Christensen, Jan P. |
author_sort | Uddbäck, Ida |
collection | PubMed |
description | Understanding the complexity of the T-cell epitope hierarchy in humans through mouse models can be difficult. In particular, using only one murine strain, the C57BL/6 mouse, to investigate the immune response to influenza virus infection limits our understanding. In the present study, by immunizing C57BL/6 mice with an adenoviral vector encoding the polymerase acidic (AdIiPA) protein of influenza A virus, we were able to induce a high number of PA-specific T cells. However, upon challenge, these cells were only partly protective. When instead immunizing BALB/c mice with AdIiPA, we found that the immunized mice were fully protected against challenge. We found that this protection was dependent on CD8 T cells, and we identified a novel H-2D(d)-restricted epitope, PA33. These findings provide a new tool for researchers to study PA-specific immunity in mice with an H-2(d) haplotype. Additionally, our findings underscore the importance of critically evaluating important limitations of using a single inbred mouse strain in vaccine studies. |
format | Online Article Text |
id | pubmed-8949235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89492352022-03-26 A Novel H-2(d) Epitope for Influenza A Polymerase Acidic Protein Uddbäck, Ida Kohlmeier, Jacob E. Thomsen, Allan R. Christensen, Jan P. Viruses Article Understanding the complexity of the T-cell epitope hierarchy in humans through mouse models can be difficult. In particular, using only one murine strain, the C57BL/6 mouse, to investigate the immune response to influenza virus infection limits our understanding. In the present study, by immunizing C57BL/6 mice with an adenoviral vector encoding the polymerase acidic (AdIiPA) protein of influenza A virus, we were able to induce a high number of PA-specific T cells. However, upon challenge, these cells were only partly protective. When instead immunizing BALB/c mice with AdIiPA, we found that the immunized mice were fully protected against challenge. We found that this protection was dependent on CD8 T cells, and we identified a novel H-2D(d)-restricted epitope, PA33. These findings provide a new tool for researchers to study PA-specific immunity in mice with an H-2(d) haplotype. Additionally, our findings underscore the importance of critically evaluating important limitations of using a single inbred mouse strain in vaccine studies. MDPI 2022-03-14 /pmc/articles/PMC8949235/ /pubmed/35337006 http://dx.doi.org/10.3390/v14030601 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Uddbäck, Ida Kohlmeier, Jacob E. Thomsen, Allan R. Christensen, Jan P. A Novel H-2(d) Epitope for Influenza A Polymerase Acidic Protein |
title | A Novel H-2(d) Epitope for Influenza A Polymerase Acidic Protein |
title_full | A Novel H-2(d) Epitope for Influenza A Polymerase Acidic Protein |
title_fullStr | A Novel H-2(d) Epitope for Influenza A Polymerase Acidic Protein |
title_full_unstemmed | A Novel H-2(d) Epitope for Influenza A Polymerase Acidic Protein |
title_short | A Novel H-2(d) Epitope for Influenza A Polymerase Acidic Protein |
title_sort | novel h-2(d) epitope for influenza a polymerase acidic protein |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949235/ https://www.ncbi.nlm.nih.gov/pubmed/35337006 http://dx.doi.org/10.3390/v14030601 |
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