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Decellularized Articular Cartilage Microgels as Microcarriers for Expansion of Mesenchymal Stem Cells

Conventional microcarriers used for expansion of human mesenchymal stem cells (hMSCs) require detachment and separation of the cells from the carrier prior to use in clinical applications for regeneration of articular cartilage, and the carrier can cause undesirable phenotypic changes in the expande...

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Detalles Bibliográficos
Autores principales: Jabbari, Esmaiel, Sepahvandi, Azadeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949257/
https://www.ncbi.nlm.nih.gov/pubmed/35323261
http://dx.doi.org/10.3390/gels8030148
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author Jabbari, Esmaiel
Sepahvandi, Azadeh
author_facet Jabbari, Esmaiel
Sepahvandi, Azadeh
author_sort Jabbari, Esmaiel
collection PubMed
description Conventional microcarriers used for expansion of human mesenchymal stem cells (hMSCs) require detachment and separation of the cells from the carrier prior to use in clinical applications for regeneration of articular cartilage, and the carrier can cause undesirable phenotypic changes in the expanded cells. This work describes a novel approach to expand hMSCs on biomimetic carriers based on adult or fetal decellularized bovine articular cartilage that supports tissue regeneration without the need to detach the expanded cells from the carrier. In this approach, the fetal or adult bovine articular cartilage was minced, decellularized, freeze-dried, ground, and sieved to produce articular cartilage microgels (CMGs) in a specified size range. Next, the hMSCs were expanded on CMGs in a bioreactor in basal medium to generate hMSC-loaded CMG microgels (CMG-MSCs). Then, the CMG-MSCs were suspended in sodium alginate, injected in a mold, crosslinked with calcium chloride, and incubated in chondrogenic medium as an injectable cellular construct for regeneration of articular cartilage. The expression of chondrogenic markers and compressive moduli of the injectable CMG-MSCs/alginate hydrogels incubated in chondrogenic medium were higher compared to the hMSCs directly encapsulated in alginate hydrogels.
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spelling pubmed-89492572022-03-26 Decellularized Articular Cartilage Microgels as Microcarriers for Expansion of Mesenchymal Stem Cells Jabbari, Esmaiel Sepahvandi, Azadeh Gels Article Conventional microcarriers used for expansion of human mesenchymal stem cells (hMSCs) require detachment and separation of the cells from the carrier prior to use in clinical applications for regeneration of articular cartilage, and the carrier can cause undesirable phenotypic changes in the expanded cells. This work describes a novel approach to expand hMSCs on biomimetic carriers based on adult or fetal decellularized bovine articular cartilage that supports tissue regeneration without the need to detach the expanded cells from the carrier. In this approach, the fetal or adult bovine articular cartilage was minced, decellularized, freeze-dried, ground, and sieved to produce articular cartilage microgels (CMGs) in a specified size range. Next, the hMSCs were expanded on CMGs in a bioreactor in basal medium to generate hMSC-loaded CMG microgels (CMG-MSCs). Then, the CMG-MSCs were suspended in sodium alginate, injected in a mold, crosslinked with calcium chloride, and incubated in chondrogenic medium as an injectable cellular construct for regeneration of articular cartilage. The expression of chondrogenic markers and compressive moduli of the injectable CMG-MSCs/alginate hydrogels incubated in chondrogenic medium were higher compared to the hMSCs directly encapsulated in alginate hydrogels. MDPI 2022-02-27 /pmc/articles/PMC8949257/ /pubmed/35323261 http://dx.doi.org/10.3390/gels8030148 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jabbari, Esmaiel
Sepahvandi, Azadeh
Decellularized Articular Cartilage Microgels as Microcarriers for Expansion of Mesenchymal Stem Cells
title Decellularized Articular Cartilage Microgels as Microcarriers for Expansion of Mesenchymal Stem Cells
title_full Decellularized Articular Cartilage Microgels as Microcarriers for Expansion of Mesenchymal Stem Cells
title_fullStr Decellularized Articular Cartilage Microgels as Microcarriers for Expansion of Mesenchymal Stem Cells
title_full_unstemmed Decellularized Articular Cartilage Microgels as Microcarriers for Expansion of Mesenchymal Stem Cells
title_short Decellularized Articular Cartilage Microgels as Microcarriers for Expansion of Mesenchymal Stem Cells
title_sort decellularized articular cartilage microgels as microcarriers for expansion of mesenchymal stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949257/
https://www.ncbi.nlm.nih.gov/pubmed/35323261
http://dx.doi.org/10.3390/gels8030148
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