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Genetic Analysis of Influenza A/H1N1pdm Strains Isolated in Bangladesh in Early 2020

Influenza is one of the most common respiratory virus infections. We analyzed hemagglutinin (HA) and neuraminidase (NA) gene segments of viruses isolated from influenza patients who visited Evercare Hospital Dhaka, Bangladesh, in early 2020 immediately before the coronavirus disease 2019 (COVID-19)...

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Autores principales: Hasan, Abu, Sasaki, Tadahiro, Phadungsombat, Juthamas, Koketsu, Ritsuko, Rahim, Rummana, Ara, Nikhat, Biswas, Suma Mita, Yonezawa, Riku, Nakayama, Emi E., Rahman, Mizanur, Shioda, Tatsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949303/
https://www.ncbi.nlm.nih.gov/pubmed/35324585
http://dx.doi.org/10.3390/tropicalmed7030038
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author Hasan, Abu
Sasaki, Tadahiro
Phadungsombat, Juthamas
Koketsu, Ritsuko
Rahim, Rummana
Ara, Nikhat
Biswas, Suma Mita
Yonezawa, Riku
Nakayama, Emi E.
Rahman, Mizanur
Shioda, Tatsuo
author_facet Hasan, Abu
Sasaki, Tadahiro
Phadungsombat, Juthamas
Koketsu, Ritsuko
Rahim, Rummana
Ara, Nikhat
Biswas, Suma Mita
Yonezawa, Riku
Nakayama, Emi E.
Rahman, Mizanur
Shioda, Tatsuo
author_sort Hasan, Abu
collection PubMed
description Influenza is one of the most common respiratory virus infections. We analyzed hemagglutinin (HA) and neuraminidase (NA) gene segments of viruses isolated from influenza patients who visited Evercare Hospital Dhaka, Bangladesh, in early 2020 immediately before the coronavirus disease 2019 (COVID-19) pandemic. All of them were influenza virus type A (IAV) H1N1pdm. Sequence analysis of the HA segments of the virus strains isolated from the clinical specimens and the subsequent phylogenic analyses of the obtained sequences revealed that all of the H1N1pdm recent subclades 6B.1A5A + 187V/A, 6B.1A5A + 156K, and 6B.1A5A + 156K with K209M were already present in Bangladesh in January 2020. Molecular clock analysis results suggested that the subclade 6B.1A5A + 156K emerged in Denmark, Australia, or the United States in July 2019, while subclades 6B.1A5A + 187V/A and 6B.1A5A + 156K with K209M emerged in East Asia in April and September 2019, respectively. On the other hand, sequence analysis of NA segments showed that the viruses lacked the H275Y mutation that confers oseltamivir resistance. Since the number of influenza cases in Bangladesh is usually small between November and January, these results indicated that the IAV H1N1pdm had spread extremely rapidly without acquiring oseltamivir resistance during a time of active international flow of people before the COVID-19 pandemic.
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spelling pubmed-89493032022-03-26 Genetic Analysis of Influenza A/H1N1pdm Strains Isolated in Bangladesh in Early 2020 Hasan, Abu Sasaki, Tadahiro Phadungsombat, Juthamas Koketsu, Ritsuko Rahim, Rummana Ara, Nikhat Biswas, Suma Mita Yonezawa, Riku Nakayama, Emi E. Rahman, Mizanur Shioda, Tatsuo Trop Med Infect Dis Article Influenza is one of the most common respiratory virus infections. We analyzed hemagglutinin (HA) and neuraminidase (NA) gene segments of viruses isolated from influenza patients who visited Evercare Hospital Dhaka, Bangladesh, in early 2020 immediately before the coronavirus disease 2019 (COVID-19) pandemic. All of them were influenza virus type A (IAV) H1N1pdm. Sequence analysis of the HA segments of the virus strains isolated from the clinical specimens and the subsequent phylogenic analyses of the obtained sequences revealed that all of the H1N1pdm recent subclades 6B.1A5A + 187V/A, 6B.1A5A + 156K, and 6B.1A5A + 156K with K209M were already present in Bangladesh in January 2020. Molecular clock analysis results suggested that the subclade 6B.1A5A + 156K emerged in Denmark, Australia, or the United States in July 2019, while subclades 6B.1A5A + 187V/A and 6B.1A5A + 156K with K209M emerged in East Asia in April and September 2019, respectively. On the other hand, sequence analysis of NA segments showed that the viruses lacked the H275Y mutation that confers oseltamivir resistance. Since the number of influenza cases in Bangladesh is usually small between November and January, these results indicated that the IAV H1N1pdm had spread extremely rapidly without acquiring oseltamivir resistance during a time of active international flow of people before the COVID-19 pandemic. MDPI 2022-02-28 /pmc/articles/PMC8949303/ /pubmed/35324585 http://dx.doi.org/10.3390/tropicalmed7030038 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hasan, Abu
Sasaki, Tadahiro
Phadungsombat, Juthamas
Koketsu, Ritsuko
Rahim, Rummana
Ara, Nikhat
Biswas, Suma Mita
Yonezawa, Riku
Nakayama, Emi E.
Rahman, Mizanur
Shioda, Tatsuo
Genetic Analysis of Influenza A/H1N1pdm Strains Isolated in Bangladesh in Early 2020
title Genetic Analysis of Influenza A/H1N1pdm Strains Isolated in Bangladesh in Early 2020
title_full Genetic Analysis of Influenza A/H1N1pdm Strains Isolated in Bangladesh in Early 2020
title_fullStr Genetic Analysis of Influenza A/H1N1pdm Strains Isolated in Bangladesh in Early 2020
title_full_unstemmed Genetic Analysis of Influenza A/H1N1pdm Strains Isolated in Bangladesh in Early 2020
title_short Genetic Analysis of Influenza A/H1N1pdm Strains Isolated in Bangladesh in Early 2020
title_sort genetic analysis of influenza a/h1n1pdm strains isolated in bangladesh in early 2020
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949303/
https://www.ncbi.nlm.nih.gov/pubmed/35324585
http://dx.doi.org/10.3390/tropicalmed7030038
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