Cargando…

SARS-CoV-2 Proteins Interact with Alpha Synuclein and Induce Lewy Body-like Pathology In Vitro

Growing cases of patients reported have shown a potential relationship between (severe acute respiratory syndrome coronavirus 2) SARS-CoV-2 infection and Parkinson’s disease (PD). However, it is unclear whether there is a molecular link between these two diseases. Alpha-synuclein (α-Syn), an aggrega...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Zhengcun, Zhang, Xiuao, Huang, Zhangqiong, Ma, Kaili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949667/
https://www.ncbi.nlm.nih.gov/pubmed/35328814
http://dx.doi.org/10.3390/ijms23063394
_version_ 1784674956342198272
author Wu, Zhengcun
Zhang, Xiuao
Huang, Zhangqiong
Ma, Kaili
author_facet Wu, Zhengcun
Zhang, Xiuao
Huang, Zhangqiong
Ma, Kaili
author_sort Wu, Zhengcun
collection PubMed
description Growing cases of patients reported have shown a potential relationship between (severe acute respiratory syndrome coronavirus 2) SARS-CoV-2 infection and Parkinson’s disease (PD). However, it is unclear whether there is a molecular link between these two diseases. Alpha-synuclein (α-Syn), an aggregation-prone protein, is considered a crucial factor in PD pathology. In this study, bioinformatics analysis confirmed favorable binding affinity between α-Syn and SARS-CoV-2 spike (S) protein and nucleocapsid (N) protein, and direct interactions were further verified in HEK293 cells. The expression of α-Syn was upregulated and its aggregation was accelerated by S protein and N protein. It was noticed that SARS-CoV-2 proteins caused Lewy-like pathology in the presence of α-Syn overexpression. By confirming that SARS-CoV-2 proteins directly interact with α-Syn, our study offered new insights into the mechanism underlying the development of PD on the background of COVID-19.
format Online
Article
Text
id pubmed-8949667
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89496672022-03-26 SARS-CoV-2 Proteins Interact with Alpha Synuclein and Induce Lewy Body-like Pathology In Vitro Wu, Zhengcun Zhang, Xiuao Huang, Zhangqiong Ma, Kaili Int J Mol Sci Article Growing cases of patients reported have shown a potential relationship between (severe acute respiratory syndrome coronavirus 2) SARS-CoV-2 infection and Parkinson’s disease (PD). However, it is unclear whether there is a molecular link between these two diseases. Alpha-synuclein (α-Syn), an aggregation-prone protein, is considered a crucial factor in PD pathology. In this study, bioinformatics analysis confirmed favorable binding affinity between α-Syn and SARS-CoV-2 spike (S) protein and nucleocapsid (N) protein, and direct interactions were further verified in HEK293 cells. The expression of α-Syn was upregulated and its aggregation was accelerated by S protein and N protein. It was noticed that SARS-CoV-2 proteins caused Lewy-like pathology in the presence of α-Syn overexpression. By confirming that SARS-CoV-2 proteins directly interact with α-Syn, our study offered new insights into the mechanism underlying the development of PD on the background of COVID-19. MDPI 2022-03-21 /pmc/articles/PMC8949667/ /pubmed/35328814 http://dx.doi.org/10.3390/ijms23063394 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Zhengcun
Zhang, Xiuao
Huang, Zhangqiong
Ma, Kaili
SARS-CoV-2 Proteins Interact with Alpha Synuclein and Induce Lewy Body-like Pathology In Vitro
title SARS-CoV-2 Proteins Interact with Alpha Synuclein and Induce Lewy Body-like Pathology In Vitro
title_full SARS-CoV-2 Proteins Interact with Alpha Synuclein and Induce Lewy Body-like Pathology In Vitro
title_fullStr SARS-CoV-2 Proteins Interact with Alpha Synuclein and Induce Lewy Body-like Pathology In Vitro
title_full_unstemmed SARS-CoV-2 Proteins Interact with Alpha Synuclein and Induce Lewy Body-like Pathology In Vitro
title_short SARS-CoV-2 Proteins Interact with Alpha Synuclein and Induce Lewy Body-like Pathology In Vitro
title_sort sars-cov-2 proteins interact with alpha synuclein and induce lewy body-like pathology in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949667/
https://www.ncbi.nlm.nih.gov/pubmed/35328814
http://dx.doi.org/10.3390/ijms23063394
work_keys_str_mv AT wuzhengcun sarscov2proteinsinteractwithalphasynucleinandinducelewybodylikepathologyinvitro
AT zhangxiuao sarscov2proteinsinteractwithalphasynucleinandinducelewybodylikepathologyinvitro
AT huangzhangqiong sarscov2proteinsinteractwithalphasynucleinandinducelewybodylikepathologyinvitro
AT makaili sarscov2proteinsinteractwithalphasynucleinandinducelewybodylikepathologyinvitro