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Effect of the PmARF6 Gene from Masson Pine (Pinus massoniana) on the Development of Arabidopsis

Masson pine (Pinus massoniana) is a core industrial tree species that is used for afforestation in southern China. Previous studies have shown that Auxin Response Factors (ARFs) are involved in the growth and development of various species, but the function of ARFs in Masson pine is unclear. In this...

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Autores principales: Ye, Youju, Han, Xin, Rong, Hao, Qian, Renjuan, Zheng, Jian, Ni, Zhouxian, Xu, Li’an
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949783/
https://www.ncbi.nlm.nih.gov/pubmed/35328022
http://dx.doi.org/10.3390/genes13030469
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author Ye, Youju
Han, Xin
Rong, Hao
Qian, Renjuan
Zheng, Jian
Ni, Zhouxian
Xu, Li’an
author_facet Ye, Youju
Han, Xin
Rong, Hao
Qian, Renjuan
Zheng, Jian
Ni, Zhouxian
Xu, Li’an
author_sort Ye, Youju
collection PubMed
description Masson pine (Pinus massoniana) is a core industrial tree species that is used for afforestation in southern China. Previous studies have shown that Auxin Response Factors (ARFs) are involved in the growth and development of various species, but the function of ARFs in Masson pine is unclear. In this research, we cloned and identified Masson pine ARF6 cDNA (PmARF6). The results showed that PmARF6 encodes a protein of 681 amino acids that is highly expressed in female flowers. Subcellular analysis showed that the PmARF6 protein occurred predominantly in the nucleus and cytomembrane of Masson pine cells. Compared with wild-type (WT) Arabidopsis, transgenic Arabidopsis plants overexpressing PmARF6 had fewer rosette leaves, and their flower development was slower. These results suggest that overexpression of PmARF6 may inhibit the flower and leaf development of Masson pine and provide new insights into the underlying developmental mechanism.
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spelling pubmed-89497832022-03-26 Effect of the PmARF6 Gene from Masson Pine (Pinus massoniana) on the Development of Arabidopsis Ye, Youju Han, Xin Rong, Hao Qian, Renjuan Zheng, Jian Ni, Zhouxian Xu, Li’an Genes (Basel) Article Masson pine (Pinus massoniana) is a core industrial tree species that is used for afforestation in southern China. Previous studies have shown that Auxin Response Factors (ARFs) are involved in the growth and development of various species, but the function of ARFs in Masson pine is unclear. In this research, we cloned and identified Masson pine ARF6 cDNA (PmARF6). The results showed that PmARF6 encodes a protein of 681 amino acids that is highly expressed in female flowers. Subcellular analysis showed that the PmARF6 protein occurred predominantly in the nucleus and cytomembrane of Masson pine cells. Compared with wild-type (WT) Arabidopsis, transgenic Arabidopsis plants overexpressing PmARF6 had fewer rosette leaves, and their flower development was slower. These results suggest that overexpression of PmARF6 may inhibit the flower and leaf development of Masson pine and provide new insights into the underlying developmental mechanism. MDPI 2022-03-07 /pmc/articles/PMC8949783/ /pubmed/35328022 http://dx.doi.org/10.3390/genes13030469 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ye, Youju
Han, Xin
Rong, Hao
Qian, Renjuan
Zheng, Jian
Ni, Zhouxian
Xu, Li’an
Effect of the PmARF6 Gene from Masson Pine (Pinus massoniana) on the Development of Arabidopsis
title Effect of the PmARF6 Gene from Masson Pine (Pinus massoniana) on the Development of Arabidopsis
title_full Effect of the PmARF6 Gene from Masson Pine (Pinus massoniana) on the Development of Arabidopsis
title_fullStr Effect of the PmARF6 Gene from Masson Pine (Pinus massoniana) on the Development of Arabidopsis
title_full_unstemmed Effect of the PmARF6 Gene from Masson Pine (Pinus massoniana) on the Development of Arabidopsis
title_short Effect of the PmARF6 Gene from Masson Pine (Pinus massoniana) on the Development of Arabidopsis
title_sort effect of the pmarf6 gene from masson pine (pinus massoniana) on the development of arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949783/
https://www.ncbi.nlm.nih.gov/pubmed/35328022
http://dx.doi.org/10.3390/genes13030469
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