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In Vitro Anthelmintic Activity of Isatis tinctoria Extracts against Ewes’ Gastrointestinal Nematodes (GINs), a Possible Application for Animal Welfare

Sheep gastrointestinal nematode (GIN) infestation represents a limiting factor for sheep farming and milk production in Italy. The development of anthelmintic resistance to conventionally used drugs suggests the path towards the use of natural remedies as a possible alternative. The purpose of this...

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Autores principales: Ragusa, Monica, Miceli, Natalizia, Piras, Cristian, Bosco, Antonio, Castagna, Fabio, Rinaldi, Laura, Musella, Vincenzo, Taviano, Maria Fernanda, Britti, Domenico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949818/
https://www.ncbi.nlm.nih.gov/pubmed/35324857
http://dx.doi.org/10.3390/vetsci9030129
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author Ragusa, Monica
Miceli, Natalizia
Piras, Cristian
Bosco, Antonio
Castagna, Fabio
Rinaldi, Laura
Musella, Vincenzo
Taviano, Maria Fernanda
Britti, Domenico
author_facet Ragusa, Monica
Miceli, Natalizia
Piras, Cristian
Bosco, Antonio
Castagna, Fabio
Rinaldi, Laura
Musella, Vincenzo
Taviano, Maria Fernanda
Britti, Domenico
author_sort Ragusa, Monica
collection PubMed
description Sheep gastrointestinal nematode (GIN) infestation represents a limiting factor for sheep farming and milk production in Italy. The development of anthelmintic resistance to conventionally used drugs suggests the path towards the use of natural remedies as a possible alternative. The purpose of this study is to evaluate the in vitro anthelmintic efficacy of the hydroalcoholic extracts of basal leaves (It-BL), cauline leaves (It-CL) and flowers (It-F) of Isatis tinctoria (Brassicaceae), a spontaneous Sicilian species renowned as an important source of bioactive compounds. The dry extracts of the different parts of the plant were tested using the egg hatch test (EHT) in vitro to verify the efficacy against ovine GIN at different concentrations (1.00, 0.5, 0.25, 0.125 mg/mL). Thiabendazole and deionized water were used as positive and negative controls, respectively. The results obtained from EHT indicated that all the I. tinctoria extracts were highly effective (p < 0.0001) in inhibiting egg hatching within 48 h of exposure. The in vitro inhibitory effect was never less than 84% in all doses tested, and it was only slightly lower than the standard drug thiabendazole (95.6%). The current study documents the anthelmintic activity of I. tinctoria against sheep’s GIN, suggesting its application as alternative natural method to limit the use of antiparasitic drugs.
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spelling pubmed-89498182022-03-26 In Vitro Anthelmintic Activity of Isatis tinctoria Extracts against Ewes’ Gastrointestinal Nematodes (GINs), a Possible Application for Animal Welfare Ragusa, Monica Miceli, Natalizia Piras, Cristian Bosco, Antonio Castagna, Fabio Rinaldi, Laura Musella, Vincenzo Taviano, Maria Fernanda Britti, Domenico Vet Sci Article Sheep gastrointestinal nematode (GIN) infestation represents a limiting factor for sheep farming and milk production in Italy. The development of anthelmintic resistance to conventionally used drugs suggests the path towards the use of natural remedies as a possible alternative. The purpose of this study is to evaluate the in vitro anthelmintic efficacy of the hydroalcoholic extracts of basal leaves (It-BL), cauline leaves (It-CL) and flowers (It-F) of Isatis tinctoria (Brassicaceae), a spontaneous Sicilian species renowned as an important source of bioactive compounds. The dry extracts of the different parts of the plant were tested using the egg hatch test (EHT) in vitro to verify the efficacy against ovine GIN at different concentrations (1.00, 0.5, 0.25, 0.125 mg/mL). Thiabendazole and deionized water were used as positive and negative controls, respectively. The results obtained from EHT indicated that all the I. tinctoria extracts were highly effective (p < 0.0001) in inhibiting egg hatching within 48 h of exposure. The in vitro inhibitory effect was never less than 84% in all doses tested, and it was only slightly lower than the standard drug thiabendazole (95.6%). The current study documents the anthelmintic activity of I. tinctoria against sheep’s GIN, suggesting its application as alternative natural method to limit the use of antiparasitic drugs. MDPI 2022-03-10 /pmc/articles/PMC8949818/ /pubmed/35324857 http://dx.doi.org/10.3390/vetsci9030129 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ragusa, Monica
Miceli, Natalizia
Piras, Cristian
Bosco, Antonio
Castagna, Fabio
Rinaldi, Laura
Musella, Vincenzo
Taviano, Maria Fernanda
Britti, Domenico
In Vitro Anthelmintic Activity of Isatis tinctoria Extracts against Ewes’ Gastrointestinal Nematodes (GINs), a Possible Application for Animal Welfare
title In Vitro Anthelmintic Activity of Isatis tinctoria Extracts against Ewes’ Gastrointestinal Nematodes (GINs), a Possible Application for Animal Welfare
title_full In Vitro Anthelmintic Activity of Isatis tinctoria Extracts against Ewes’ Gastrointestinal Nematodes (GINs), a Possible Application for Animal Welfare
title_fullStr In Vitro Anthelmintic Activity of Isatis tinctoria Extracts against Ewes’ Gastrointestinal Nematodes (GINs), a Possible Application for Animal Welfare
title_full_unstemmed In Vitro Anthelmintic Activity of Isatis tinctoria Extracts against Ewes’ Gastrointestinal Nematodes (GINs), a Possible Application for Animal Welfare
title_short In Vitro Anthelmintic Activity of Isatis tinctoria Extracts against Ewes’ Gastrointestinal Nematodes (GINs), a Possible Application for Animal Welfare
title_sort in vitro anthelmintic activity of isatis tinctoria extracts against ewes’ gastrointestinal nematodes (gins), a possible application for animal welfare
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949818/
https://www.ncbi.nlm.nih.gov/pubmed/35324857
http://dx.doi.org/10.3390/vetsci9030129
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