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The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes
In view of the possible medical applications of saponins, the molecular structure of a GOTCAB saponin from the roots of Gypsophila paniculata L. was determined by NMR. The biological activity of saponins may depend on the interaction with cell membranes. To obtain more insight in the mechanism of me...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949875/ https://www.ncbi.nlm.nih.gov/pubmed/35328818 http://dx.doi.org/10.3390/ijms23063397 |
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author | Korchowiec, Beata Korchowiec, Jacek Kwiecińska, Klaudia Gevrenova, Reneta Bouguet-Bonnet, Sabine Deng, Cheng Henry, Max Rogalska, Ewa |
author_facet | Korchowiec, Beata Korchowiec, Jacek Kwiecińska, Klaudia Gevrenova, Reneta Bouguet-Bonnet, Sabine Deng, Cheng Henry, Max Rogalska, Ewa |
author_sort | Korchowiec, Beata |
collection | PubMed |
description | In view of the possible medical applications of saponins, the molecular structure of a GOTCAB saponin from the roots of Gypsophila paniculata L. was determined by NMR. The biological activity of saponins may depend on the interaction with cell membranes. To obtain more insight in the mechanism of membrane-related saponin function, an experimental and theoretical study was conducted. Ternary lipid systems composed of sphingomyelin, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, and cholesterol were used as models of mammalian cell membranes. The membrane–saponin interaction was studied experimentally by monitoring surface pressure in the monomolecular films formed at the air–aqueous subphase interface. The behavior of GOTCAB saponin in a water box and model monolayer systems was characterized by molecular dynamics simulations. The results obtained showed that, in the systems used, cholesterol had a decisive effect on the interaction between GOTCAB and phosphocholine or sphingomyelin as well as on its location within the lipid film. |
format | Online Article Text |
id | pubmed-8949875 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89498752022-03-26 The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes Korchowiec, Beata Korchowiec, Jacek Kwiecińska, Klaudia Gevrenova, Reneta Bouguet-Bonnet, Sabine Deng, Cheng Henry, Max Rogalska, Ewa Int J Mol Sci Article In view of the possible medical applications of saponins, the molecular structure of a GOTCAB saponin from the roots of Gypsophila paniculata L. was determined by NMR. The biological activity of saponins may depend on the interaction with cell membranes. To obtain more insight in the mechanism of membrane-related saponin function, an experimental and theoretical study was conducted. Ternary lipid systems composed of sphingomyelin, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, and cholesterol were used as models of mammalian cell membranes. The membrane–saponin interaction was studied experimentally by monitoring surface pressure in the monomolecular films formed at the air–aqueous subphase interface. The behavior of GOTCAB saponin in a water box and model monolayer systems was characterized by molecular dynamics simulations. The results obtained showed that, in the systems used, cholesterol had a decisive effect on the interaction between GOTCAB and phosphocholine or sphingomyelin as well as on its location within the lipid film. MDPI 2022-03-21 /pmc/articles/PMC8949875/ /pubmed/35328818 http://dx.doi.org/10.3390/ijms23063397 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Korchowiec, Beata Korchowiec, Jacek Kwiecińska, Klaudia Gevrenova, Reneta Bouguet-Bonnet, Sabine Deng, Cheng Henry, Max Rogalska, Ewa The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes |
title | The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes |
title_full | The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes |
title_fullStr | The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes |
title_full_unstemmed | The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes |
title_short | The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes |
title_sort | molecular bases of the interaction between a saponin from the roots of gypsophila paniculata l. and model lipid membranes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8949875/ https://www.ncbi.nlm.nih.gov/pubmed/35328818 http://dx.doi.org/10.3390/ijms23063397 |
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