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Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study

(1) Background: To investigate the effect of a xenogeneic collagen matrix (CMX) seeded with autologous gingiva-derived mesenchymal cells (GMSCs) when combined with a coronally advanced flap (CAF) in the treatment of localized gingival recession type 1 (RT1). (2) Methods: Dehiscence-type defects were...

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Autores principales: Sanchez, Nerea, Vignoletti, Fabio, Sanz-Martin, Ignacio, Coca, Alejandro, Nuñez, Javier, Maldonado, Estela, Sanz-Esporrin, Javier, Hernando-Pradíes, Irene, Santamaría, Silvia, Herrera, David, Garcia-Sanz, Jose A., Sanz, Mariano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950013/
https://www.ncbi.nlm.nih.gov/pubmed/35328670
http://dx.doi.org/10.3390/ijms23063248
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author Sanchez, Nerea
Vignoletti, Fabio
Sanz-Martin, Ignacio
Coca, Alejandro
Nuñez, Javier
Maldonado, Estela
Sanz-Esporrin, Javier
Hernando-Pradíes, Irene
Santamaría, Silvia
Herrera, David
Garcia-Sanz, Jose A.
Sanz, Mariano
author_facet Sanchez, Nerea
Vignoletti, Fabio
Sanz-Martin, Ignacio
Coca, Alejandro
Nuñez, Javier
Maldonado, Estela
Sanz-Esporrin, Javier
Hernando-Pradíes, Irene
Santamaría, Silvia
Herrera, David
Garcia-Sanz, Jose A.
Sanz, Mariano
author_sort Sanchez, Nerea
collection PubMed
description (1) Background: To investigate the effect of a xenogeneic collagen matrix (CMX) seeded with autologous gingiva-derived mesenchymal cells (GMSCs) when combined with a coronally advanced flap (CAF) in the treatment of localized gingival recession type 1 (RT1). (2) Methods: Dehiscence-type defects were created in seven dogs. GMSCs were isolated, transfected with a vector carrying green fluorescent protein (GFP) and expanded. Once chronified, the defects were randomly treated with (1) CAF plus the combination of CMX and GFP(+) GMSCs, (2) CAF plus CMX with autologous fibroblasts, (3) CAF plus CMX and (4) CAF alone. Histological and clinical outcomes at 2- and 6-week healing periods were analyzed and compared among groups. (3) Results: Histologically, the addition of autologous cells to the CMX resulted in reduced inflammation and a variable degree of new cementum/bone formation. CMX plus GMSCs resulted in greater mean recession reduction (1.42; SD = 1.88 mm) and percentage of teeth with recession reduction of ≥2 mm (57%) when compared to the other groups, although these differences were not statistically significant. (4) Conclusions: The histometric and clinical results indicated a positive trend favouring the combination of CMX and GMSCs with the CAF when compared to the groups without cells, although these differences were not statistically significant.
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spelling pubmed-89500132022-03-26 Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study Sanchez, Nerea Vignoletti, Fabio Sanz-Martin, Ignacio Coca, Alejandro Nuñez, Javier Maldonado, Estela Sanz-Esporrin, Javier Hernando-Pradíes, Irene Santamaría, Silvia Herrera, David Garcia-Sanz, Jose A. Sanz, Mariano Int J Mol Sci Article (1) Background: To investigate the effect of a xenogeneic collagen matrix (CMX) seeded with autologous gingiva-derived mesenchymal cells (GMSCs) when combined with a coronally advanced flap (CAF) in the treatment of localized gingival recession type 1 (RT1). (2) Methods: Dehiscence-type defects were created in seven dogs. GMSCs were isolated, transfected with a vector carrying green fluorescent protein (GFP) and expanded. Once chronified, the defects were randomly treated with (1) CAF plus the combination of CMX and GFP(+) GMSCs, (2) CAF plus CMX with autologous fibroblasts, (3) CAF plus CMX and (4) CAF alone. Histological and clinical outcomes at 2- and 6-week healing periods were analyzed and compared among groups. (3) Results: Histologically, the addition of autologous cells to the CMX resulted in reduced inflammation and a variable degree of new cementum/bone formation. CMX plus GMSCs resulted in greater mean recession reduction (1.42; SD = 1.88 mm) and percentage of teeth with recession reduction of ≥2 mm (57%) when compared to the other groups, although these differences were not statistically significant. (4) Conclusions: The histometric and clinical results indicated a positive trend favouring the combination of CMX and GMSCs with the CAF when compared to the groups without cells, although these differences were not statistically significant. MDPI 2022-03-17 /pmc/articles/PMC8950013/ /pubmed/35328670 http://dx.doi.org/10.3390/ijms23063248 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sanchez, Nerea
Vignoletti, Fabio
Sanz-Martin, Ignacio
Coca, Alejandro
Nuñez, Javier
Maldonado, Estela
Sanz-Esporrin, Javier
Hernando-Pradíes, Irene
Santamaría, Silvia
Herrera, David
Garcia-Sanz, Jose A.
Sanz, Mariano
Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study
title Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study
title_full Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study
title_fullStr Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study
title_full_unstemmed Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study
title_short Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study
title_sort cell therapy based on gingiva-derived mesenchymal stem cells seeded in a xenogeneic collagen matrix for root coverage of rt1 gingival lesions: an in vivo experimental study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950013/
https://www.ncbi.nlm.nih.gov/pubmed/35328670
http://dx.doi.org/10.3390/ijms23063248
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