Cargando…
Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study
(1) Background: To investigate the effect of a xenogeneic collagen matrix (CMX) seeded with autologous gingiva-derived mesenchymal cells (GMSCs) when combined with a coronally advanced flap (CAF) in the treatment of localized gingival recession type 1 (RT1). (2) Methods: Dehiscence-type defects were...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950013/ https://www.ncbi.nlm.nih.gov/pubmed/35328670 http://dx.doi.org/10.3390/ijms23063248 |
_version_ | 1784675041664827392 |
---|---|
author | Sanchez, Nerea Vignoletti, Fabio Sanz-Martin, Ignacio Coca, Alejandro Nuñez, Javier Maldonado, Estela Sanz-Esporrin, Javier Hernando-Pradíes, Irene Santamaría, Silvia Herrera, David Garcia-Sanz, Jose A. Sanz, Mariano |
author_facet | Sanchez, Nerea Vignoletti, Fabio Sanz-Martin, Ignacio Coca, Alejandro Nuñez, Javier Maldonado, Estela Sanz-Esporrin, Javier Hernando-Pradíes, Irene Santamaría, Silvia Herrera, David Garcia-Sanz, Jose A. Sanz, Mariano |
author_sort | Sanchez, Nerea |
collection | PubMed |
description | (1) Background: To investigate the effect of a xenogeneic collagen matrix (CMX) seeded with autologous gingiva-derived mesenchymal cells (GMSCs) when combined with a coronally advanced flap (CAF) in the treatment of localized gingival recession type 1 (RT1). (2) Methods: Dehiscence-type defects were created in seven dogs. GMSCs were isolated, transfected with a vector carrying green fluorescent protein (GFP) and expanded. Once chronified, the defects were randomly treated with (1) CAF plus the combination of CMX and GFP(+) GMSCs, (2) CAF plus CMX with autologous fibroblasts, (3) CAF plus CMX and (4) CAF alone. Histological and clinical outcomes at 2- and 6-week healing periods were analyzed and compared among groups. (3) Results: Histologically, the addition of autologous cells to the CMX resulted in reduced inflammation and a variable degree of new cementum/bone formation. CMX plus GMSCs resulted in greater mean recession reduction (1.42; SD = 1.88 mm) and percentage of teeth with recession reduction of ≥2 mm (57%) when compared to the other groups, although these differences were not statistically significant. (4) Conclusions: The histometric and clinical results indicated a positive trend favouring the combination of CMX and GMSCs with the CAF when compared to the groups without cells, although these differences were not statistically significant. |
format | Online Article Text |
id | pubmed-8950013 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89500132022-03-26 Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study Sanchez, Nerea Vignoletti, Fabio Sanz-Martin, Ignacio Coca, Alejandro Nuñez, Javier Maldonado, Estela Sanz-Esporrin, Javier Hernando-Pradíes, Irene Santamaría, Silvia Herrera, David Garcia-Sanz, Jose A. Sanz, Mariano Int J Mol Sci Article (1) Background: To investigate the effect of a xenogeneic collagen matrix (CMX) seeded with autologous gingiva-derived mesenchymal cells (GMSCs) when combined with a coronally advanced flap (CAF) in the treatment of localized gingival recession type 1 (RT1). (2) Methods: Dehiscence-type defects were created in seven dogs. GMSCs were isolated, transfected with a vector carrying green fluorescent protein (GFP) and expanded. Once chronified, the defects were randomly treated with (1) CAF plus the combination of CMX and GFP(+) GMSCs, (2) CAF plus CMX with autologous fibroblasts, (3) CAF plus CMX and (4) CAF alone. Histological and clinical outcomes at 2- and 6-week healing periods were analyzed and compared among groups. (3) Results: Histologically, the addition of autologous cells to the CMX resulted in reduced inflammation and a variable degree of new cementum/bone formation. CMX plus GMSCs resulted in greater mean recession reduction (1.42; SD = 1.88 mm) and percentage of teeth with recession reduction of ≥2 mm (57%) when compared to the other groups, although these differences were not statistically significant. (4) Conclusions: The histometric and clinical results indicated a positive trend favouring the combination of CMX and GMSCs with the CAF when compared to the groups without cells, although these differences were not statistically significant. MDPI 2022-03-17 /pmc/articles/PMC8950013/ /pubmed/35328670 http://dx.doi.org/10.3390/ijms23063248 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sanchez, Nerea Vignoletti, Fabio Sanz-Martin, Ignacio Coca, Alejandro Nuñez, Javier Maldonado, Estela Sanz-Esporrin, Javier Hernando-Pradíes, Irene Santamaría, Silvia Herrera, David Garcia-Sanz, Jose A. Sanz, Mariano Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study |
title | Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study |
title_full | Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study |
title_fullStr | Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study |
title_full_unstemmed | Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study |
title_short | Cell Therapy Based on Gingiva-Derived Mesenchymal Stem Cells Seeded in a Xenogeneic Collagen Matrix for Root Coverage of RT1 Gingival Lesions: An In Vivo Experimental Study |
title_sort | cell therapy based on gingiva-derived mesenchymal stem cells seeded in a xenogeneic collagen matrix for root coverage of rt1 gingival lesions: an in vivo experimental study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950013/ https://www.ncbi.nlm.nih.gov/pubmed/35328670 http://dx.doi.org/10.3390/ijms23063248 |
work_keys_str_mv | AT sancheznerea celltherapybasedongingivaderivedmesenchymalstemcellsseededinaxenogeneiccollagenmatrixforrootcoverageofrt1gingivallesionsaninvivoexperimentalstudy AT vignolettifabio celltherapybasedongingivaderivedmesenchymalstemcellsseededinaxenogeneiccollagenmatrixforrootcoverageofrt1gingivallesionsaninvivoexperimentalstudy AT sanzmartinignacio celltherapybasedongingivaderivedmesenchymalstemcellsseededinaxenogeneiccollagenmatrixforrootcoverageofrt1gingivallesionsaninvivoexperimentalstudy AT cocaalejandro celltherapybasedongingivaderivedmesenchymalstemcellsseededinaxenogeneiccollagenmatrixforrootcoverageofrt1gingivallesionsaninvivoexperimentalstudy AT nunezjavier celltherapybasedongingivaderivedmesenchymalstemcellsseededinaxenogeneiccollagenmatrixforrootcoverageofrt1gingivallesionsaninvivoexperimentalstudy AT maldonadoestela celltherapybasedongingivaderivedmesenchymalstemcellsseededinaxenogeneiccollagenmatrixforrootcoverageofrt1gingivallesionsaninvivoexperimentalstudy AT sanzesporrinjavier celltherapybasedongingivaderivedmesenchymalstemcellsseededinaxenogeneiccollagenmatrixforrootcoverageofrt1gingivallesionsaninvivoexperimentalstudy AT hernandopradiesirene celltherapybasedongingivaderivedmesenchymalstemcellsseededinaxenogeneiccollagenmatrixforrootcoverageofrt1gingivallesionsaninvivoexperimentalstudy AT santamariasilvia celltherapybasedongingivaderivedmesenchymalstemcellsseededinaxenogeneiccollagenmatrixforrootcoverageofrt1gingivallesionsaninvivoexperimentalstudy AT herreradavid celltherapybasedongingivaderivedmesenchymalstemcellsseededinaxenogeneiccollagenmatrixforrootcoverageofrt1gingivallesionsaninvivoexperimentalstudy AT garciasanzjosea celltherapybasedongingivaderivedmesenchymalstemcellsseededinaxenogeneiccollagenmatrixforrootcoverageofrt1gingivallesionsaninvivoexperimentalstudy AT sanzmariano celltherapybasedongingivaderivedmesenchymalstemcellsseededinaxenogeneiccollagenmatrixforrootcoverageofrt1gingivallesionsaninvivoexperimentalstudy |