Cargando…

Stabilization of Silver Nanoparticles on Polyester Fabric Using Organo-Matrices for Controlled Antimicrobial Performance

Antimicrobial textiles are helpful tools to fight against multidrug-resistant pathogens and nosocomial infections. The deposition of silver nanoparticles (AgNPs) onto textiles has been studied to achieve antimicrobial properties. Yet, due to health and environmental safety concerns associated with s...

Descripción completa

Detalles Bibliográficos
Autores principales: Ribeiro, Ana Isabel, Shvalya, Vasyl, Cvelbar, Uroš, Silva, Renata, Marques-Oliveira, Rita, Remião, Fernando, Felgueiras, Helena P., Padrão, Jorge, Zille, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950105/
https://www.ncbi.nlm.nih.gov/pubmed/35335469
http://dx.doi.org/10.3390/polym14061138
_version_ 1784675061885566976
author Ribeiro, Ana Isabel
Shvalya, Vasyl
Cvelbar, Uroš
Silva, Renata
Marques-Oliveira, Rita
Remião, Fernando
Felgueiras, Helena P.
Padrão, Jorge
Zille, Andrea
author_facet Ribeiro, Ana Isabel
Shvalya, Vasyl
Cvelbar, Uroš
Silva, Renata
Marques-Oliveira, Rita
Remião, Fernando
Felgueiras, Helena P.
Padrão, Jorge
Zille, Andrea
author_sort Ribeiro, Ana Isabel
collection PubMed
description Antimicrobial textiles are helpful tools to fight against multidrug-resistant pathogens and nosocomial infections. The deposition of silver nanoparticles (AgNPs) onto textiles has been studied to achieve antimicrobial properties. Yet, due to health and environmental safety concerns associated with such formulations, processing optimizations have been introduced: biocompatible materials, environmentally friendly agents, and delivery platforms that ensure a controlled release. In particular, the functionalization of polyester (PES) fabric with antimicrobial agents is a formulation in high demand in medical textiles. However, the lack of functional groups on PES fabric hinders the development of cost-effective, durable systems that allow a controlled release of antimicrobial agents. In this work, PES fabric was functionalized with AgNPs using one or two biocompatible layers of chitosan or hexamethyldisiloxane (HMDSO). The addition of organo-matrices stabilized the AgNPs onto the fabrics, protected AgNPs from further oxidation, and controlled their release. In addition, the layered samples were efficient against Staphylococcus aureus and Escherichia coli. The sample with two layers of chitosan showed the highest efficacy against S. aureus (log reduction of 2.15 ± 1.08 after 3 h of contact). Against E. coli, the sample with two layers of chitosan showed the best properties. Chitosan allowed to control the antimicrobial activity of AgNPs, avoid the complete loss of AgNPs after washings and act in synergy with AgNPs. After 3 h of incubation, this sample presented a log reduction of 4.81, and 7.27 of log reduction after 5 h of incubation. The antimicrobial results after washing showed a log reduction of 3.47 and 4.88 after 3 h and 5 h of contact, respectively. Furthermore, the sample with a final layer of HMDSO also presented a controlled antimicrobial effect. The antimicrobial effect was slower than the sample with just an initial layer of HMDSO, with a log reduction of 4.40 after 3 h of incubation (instead of 7.22) and 7.27 after 5 h. The biocompatibility of the composites was confirmed through the evaluation of their cytotoxicity towards HaCaT cells (cells viability > 96% in all samples). Therefore, the produced nanocomposites could have interesting applications in medical textiles once they present controlled antimicrobial properties, high biocompatibility and avoid the complete release of AgNPs to the environment.
format Online
Article
Text
id pubmed-8950105
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89501052022-03-26 Stabilization of Silver Nanoparticles on Polyester Fabric Using Organo-Matrices for Controlled Antimicrobial Performance Ribeiro, Ana Isabel Shvalya, Vasyl Cvelbar, Uroš Silva, Renata Marques-Oliveira, Rita Remião, Fernando Felgueiras, Helena P. Padrão, Jorge Zille, Andrea Polymers (Basel) Article Antimicrobial textiles are helpful tools to fight against multidrug-resistant pathogens and nosocomial infections. The deposition of silver nanoparticles (AgNPs) onto textiles has been studied to achieve antimicrobial properties. Yet, due to health and environmental safety concerns associated with such formulations, processing optimizations have been introduced: biocompatible materials, environmentally friendly agents, and delivery platforms that ensure a controlled release. In particular, the functionalization of polyester (PES) fabric with antimicrobial agents is a formulation in high demand in medical textiles. However, the lack of functional groups on PES fabric hinders the development of cost-effective, durable systems that allow a controlled release of antimicrobial agents. In this work, PES fabric was functionalized with AgNPs using one or two biocompatible layers of chitosan or hexamethyldisiloxane (HMDSO). The addition of organo-matrices stabilized the AgNPs onto the fabrics, protected AgNPs from further oxidation, and controlled their release. In addition, the layered samples were efficient against Staphylococcus aureus and Escherichia coli. The sample with two layers of chitosan showed the highest efficacy against S. aureus (log reduction of 2.15 ± 1.08 after 3 h of contact). Against E. coli, the sample with two layers of chitosan showed the best properties. Chitosan allowed to control the antimicrobial activity of AgNPs, avoid the complete loss of AgNPs after washings and act in synergy with AgNPs. After 3 h of incubation, this sample presented a log reduction of 4.81, and 7.27 of log reduction after 5 h of incubation. The antimicrobial results after washing showed a log reduction of 3.47 and 4.88 after 3 h and 5 h of contact, respectively. Furthermore, the sample with a final layer of HMDSO also presented a controlled antimicrobial effect. The antimicrobial effect was slower than the sample with just an initial layer of HMDSO, with a log reduction of 4.40 after 3 h of incubation (instead of 7.22) and 7.27 after 5 h. The biocompatibility of the composites was confirmed through the evaluation of their cytotoxicity towards HaCaT cells (cells viability > 96% in all samples). Therefore, the produced nanocomposites could have interesting applications in medical textiles once they present controlled antimicrobial properties, high biocompatibility and avoid the complete release of AgNPs to the environment. MDPI 2022-03-12 /pmc/articles/PMC8950105/ /pubmed/35335469 http://dx.doi.org/10.3390/polym14061138 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ribeiro, Ana Isabel
Shvalya, Vasyl
Cvelbar, Uroš
Silva, Renata
Marques-Oliveira, Rita
Remião, Fernando
Felgueiras, Helena P.
Padrão, Jorge
Zille, Andrea
Stabilization of Silver Nanoparticles on Polyester Fabric Using Organo-Matrices for Controlled Antimicrobial Performance
title Stabilization of Silver Nanoparticles on Polyester Fabric Using Organo-Matrices for Controlled Antimicrobial Performance
title_full Stabilization of Silver Nanoparticles on Polyester Fabric Using Organo-Matrices for Controlled Antimicrobial Performance
title_fullStr Stabilization of Silver Nanoparticles on Polyester Fabric Using Organo-Matrices for Controlled Antimicrobial Performance
title_full_unstemmed Stabilization of Silver Nanoparticles on Polyester Fabric Using Organo-Matrices for Controlled Antimicrobial Performance
title_short Stabilization of Silver Nanoparticles on Polyester Fabric Using Organo-Matrices for Controlled Antimicrobial Performance
title_sort stabilization of silver nanoparticles on polyester fabric using organo-matrices for controlled antimicrobial performance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950105/
https://www.ncbi.nlm.nih.gov/pubmed/35335469
http://dx.doi.org/10.3390/polym14061138
work_keys_str_mv AT ribeiroanaisabel stabilizationofsilvernanoparticlesonpolyesterfabricusingorganomatricesforcontrolledantimicrobialperformance
AT shvalyavasyl stabilizationofsilvernanoparticlesonpolyesterfabricusingorganomatricesforcontrolledantimicrobialperformance
AT cvelbaruros stabilizationofsilvernanoparticlesonpolyesterfabricusingorganomatricesforcontrolledantimicrobialperformance
AT silvarenata stabilizationofsilvernanoparticlesonpolyesterfabricusingorganomatricesforcontrolledantimicrobialperformance
AT marquesoliveirarita stabilizationofsilvernanoparticlesonpolyesterfabricusingorganomatricesforcontrolledantimicrobialperformance
AT remiaofernando stabilizationofsilvernanoparticlesonpolyesterfabricusingorganomatricesforcontrolledantimicrobialperformance
AT felgueirashelenap stabilizationofsilvernanoparticlesonpolyesterfabricusingorganomatricesforcontrolledantimicrobialperformance
AT padraojorge stabilizationofsilvernanoparticlesonpolyesterfabricusingorganomatricesforcontrolledantimicrobialperformance
AT zilleandrea stabilizationofsilvernanoparticlesonpolyesterfabricusingorganomatricesforcontrolledantimicrobialperformance