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Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules

The genus Bidens a member of family Compositae, is widely documented as an ethno-medicinally important genus of plants. In the present study, anticancer potential of three ethno-medicinally important species i.e., B. bipinnata, B. biternata and B. pilosa were tested. For in-vitro evaluation, an MTT...

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Autores principales: Zahara, Kulsoom, Bibi, Yamin, Masood, Saadia, Nisa, Sobia, Sher, Ahmad, Ali, Naushad, Kumar, Sunjeet, Qayyum, Abdul, Ahmed, Waseem, Sami, Rokayya, Al-Mushhin, Amina A. M., Aljahani, Amani H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950169/
https://www.ncbi.nlm.nih.gov/pubmed/35335292
http://dx.doi.org/10.3390/molecules27061927
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author Zahara, Kulsoom
Bibi, Yamin
Masood, Saadia
Nisa, Sobia
Sher, Ahmad
Ali, Naushad
Kumar, Sunjeet
Qayyum, Abdul
Ahmed, Waseem
Sami, Rokayya
Al-Mushhin, Amina A. M.
Aljahani, Amani H.
author_facet Zahara, Kulsoom
Bibi, Yamin
Masood, Saadia
Nisa, Sobia
Sher, Ahmad
Ali, Naushad
Kumar, Sunjeet
Qayyum, Abdul
Ahmed, Waseem
Sami, Rokayya
Al-Mushhin, Amina A. M.
Aljahani, Amani H.
author_sort Zahara, Kulsoom
collection PubMed
description The genus Bidens a member of family Compositae, is widely documented as an ethno-medicinally important genus of plants. In the present study, anticancer potential of three ethno-medicinally important species i.e., B. bipinnata, B. biternata and B. pilosa were tested. For in-vitro evaluation, an MTT (Thiazolyl blue tetrazolium bromide) assay was performed against cervical cancer cells (HeLa), hepatocellular carcinoma (HepG), and adenocarcinoma human alveolar basal epithelial cells (A549). For in vivo evaluation, Artemia salina, Danio rerio, and Caenorhabditis elegans were used. Among all the tested extracts, the ethanol extract of B. biternata appeared to have highest anticancer activity, and the compounds responsible for this activity were identified to be Tris (2,4-di-tert-butylphenyl), 4-hydroxy-2,4′-dimethoxychalcone, and 2,4-di-tert-butylphenol. This is the first report of the isolation of Tris (2,4-di-tert-butylphenyl) phosphate from the genus Bidens and the first report of 4-hydroxy-2,4′-dimethoxychalcone and 2,4-di-tert-butylphenol from B. biternata. Among the isolated compounds, 4-hydroxy-2,4′-dimethoxychalcone showed the highest anticancer activity with an LD50 value of 236.7 µg/mL. Therefore, this compound carries promising potential for being established as a pharmaceutical for chemoprevention and chemotherapy.
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spelling pubmed-89501692022-03-26 Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules Zahara, Kulsoom Bibi, Yamin Masood, Saadia Nisa, Sobia Sher, Ahmad Ali, Naushad Kumar, Sunjeet Qayyum, Abdul Ahmed, Waseem Sami, Rokayya Al-Mushhin, Amina A. M. Aljahani, Amani H. Molecules Article The genus Bidens a member of family Compositae, is widely documented as an ethno-medicinally important genus of plants. In the present study, anticancer potential of three ethno-medicinally important species i.e., B. bipinnata, B. biternata and B. pilosa were tested. For in-vitro evaluation, an MTT (Thiazolyl blue tetrazolium bromide) assay was performed against cervical cancer cells (HeLa), hepatocellular carcinoma (HepG), and adenocarcinoma human alveolar basal epithelial cells (A549). For in vivo evaluation, Artemia salina, Danio rerio, and Caenorhabditis elegans were used. Among all the tested extracts, the ethanol extract of B. biternata appeared to have highest anticancer activity, and the compounds responsible for this activity were identified to be Tris (2,4-di-tert-butylphenyl), 4-hydroxy-2,4′-dimethoxychalcone, and 2,4-di-tert-butylphenol. This is the first report of the isolation of Tris (2,4-di-tert-butylphenyl) phosphate from the genus Bidens and the first report of 4-hydroxy-2,4′-dimethoxychalcone and 2,4-di-tert-butylphenol from B. biternata. Among the isolated compounds, 4-hydroxy-2,4′-dimethoxychalcone showed the highest anticancer activity with an LD50 value of 236.7 µg/mL. Therefore, this compound carries promising potential for being established as a pharmaceutical for chemoprevention and chemotherapy. MDPI 2022-03-16 /pmc/articles/PMC8950169/ /pubmed/35335292 http://dx.doi.org/10.3390/molecules27061927 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zahara, Kulsoom
Bibi, Yamin
Masood, Saadia
Nisa, Sobia
Sher, Ahmad
Ali, Naushad
Kumar, Sunjeet
Qayyum, Abdul
Ahmed, Waseem
Sami, Rokayya
Al-Mushhin, Amina A. M.
Aljahani, Amani H.
Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules
title Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules
title_full Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules
title_fullStr Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules
title_full_unstemmed Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules
title_short Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules
title_sort isolation and identification of bioactive compounds from bidens spp. using hplc-dad and gc-ms analysis and their biological activity as anticancer molecules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950169/
https://www.ncbi.nlm.nih.gov/pubmed/35335292
http://dx.doi.org/10.3390/molecules27061927
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