Establishment, Genetic Diversity, and Habitat Suitability of Aedes albopictus Populations from Ecuador

SIMPLE SUMMARY: The tiger mosquito, Aedes albopictus, is a vector of multiple viral diseases. Therefore, it is crucial to understand its distribution and the genetic diversity of Ecuadorian populations. This study used a genetic marker to understand aspects of the dynamics of the Ecuadorian populations of Aedes albopictus from five sites along the coastal, Amazon basin, and Andean lowland regions. Our results evidence two haplotypes within the Ecuadorian populations. Haplotype 1 was found in the coastal regions and Amazon basin, while haplotype 2 was found in the northeastern lowlands. Worldwide, haplotype 1 has been found in 21 countries in temperate and tropical habitats, and haplotype 2 has been found in five countries in tropical habitats. Hence, a difference in adaptation traits could be speculated between both haplotypes. Our study presents a prediction model that shows the suitable habitats for Aedes albopictus in Ecuador. Our results showed that the vector could spread through all country regions, including the Galapagos Islands. Thus, understanding the different aspects of the local populations can help establish better vector surveillance and control strategies. ABSTRACT: Aedes albopictus, also known as the tiger mosquito, is widespread worldwide across tropical, subtropical, and temperate regions. This insect is associated with the transmission of several vector-borne diseases, and, as such, monitoring its distribution is highly important for public health. In Ecuador, Ae. albopictus was first reported in 2017 in Guayaquil. Since then, the vector has been identified in the Northeastern lowlands and the Amazon basin. This study aims to determine the genetic diversity of Ecuadorian populations of Ae. albopictus through the analysis of the mitochondrial gene COI and to describe the potential distribution areas of this species within the country. The genetic diversity was determined by combining phylogenetic and population genetics analyses of five localities in Ecuador. Results showed two haplotypes in the Ecuadorian populations of Ae. albopictus. Haplotype 1 (H1) was found in the coastal and Amazon individuals, while haplotype 2 (H2) was only found in the three northeastern lowlands sites. In a worldwide context, H1 is the most widespread in 21 countries with temperate and tropical habitats. In contrast, H2 distribution is limited to five countries in tropical regions, suggesting fewer adaptation traits. Our prediction model showed a suitable habitat for Ae. albopictus in all regions (coastal, Amazon basin, and Andean lowland regions and the Galápagos Islands) of Ecuador. Hence, understanding different aspects of the vector can help us implement better control strategies for surveillance and vectorial control in Ecuador.