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Microfluidic Organ-on-a-Chip Devices for Liver Disease Modeling In Vitro

Mortality from liver disease conditions continues to be very high. As liver diseases manifest and progress silently, prompt measures after diagnosis are essential in the treatment of these conditions. Microfluidic organs-on-chip platforms have significant potential for the study of the pathophysiolo...

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Autores principales: Kanabekova, Perizat, Kadyrova, Adina, Kulsharova, Gulsim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950395/
https://www.ncbi.nlm.nih.gov/pubmed/35334720
http://dx.doi.org/10.3390/mi13030428
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author Kanabekova, Perizat
Kadyrova, Adina
Kulsharova, Gulsim
author_facet Kanabekova, Perizat
Kadyrova, Adina
Kulsharova, Gulsim
author_sort Kanabekova, Perizat
collection PubMed
description Mortality from liver disease conditions continues to be very high. As liver diseases manifest and progress silently, prompt measures after diagnosis are essential in the treatment of these conditions. Microfluidic organs-on-chip platforms have significant potential for the study of the pathophysiology of liver diseases in vitro. Different liver-on-a-chip microphysiological platforms have been reported to study cell-signaling pathways such as those activating stellate cells within liver diseases. Moreover, the drug efficacy for liver conditions might be evaluated on a cellular metabolic level. Here, we present a comprehensive review of microphysiological platforms used for modelling liver diseases. First, we briefly introduce the concept and importance of organs-on-a-chip in studying liver diseases in vitro, reflecting on existing reviews of healthy liver-on-a-chip platforms. Second, the techniques of cell cultures used in the microfluidic devices, including 2D, 3D, and spheroid cells, are explained. Next, the types of liver diseases (NAFLD, ALD, hepatitis infections, and drug injury) on-chip are explained for a further comprehensive overview of the design and methods of developing liver diseases in vitro. Finally, some challenges in design and existing solutions to them are reviewed
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spelling pubmed-89503952022-03-26 Microfluidic Organ-on-a-Chip Devices for Liver Disease Modeling In Vitro Kanabekova, Perizat Kadyrova, Adina Kulsharova, Gulsim Micromachines (Basel) Review Mortality from liver disease conditions continues to be very high. As liver diseases manifest and progress silently, prompt measures after diagnosis are essential in the treatment of these conditions. Microfluidic organs-on-chip platforms have significant potential for the study of the pathophysiology of liver diseases in vitro. Different liver-on-a-chip microphysiological platforms have been reported to study cell-signaling pathways such as those activating stellate cells within liver diseases. Moreover, the drug efficacy for liver conditions might be evaluated on a cellular metabolic level. Here, we present a comprehensive review of microphysiological platforms used for modelling liver diseases. First, we briefly introduce the concept and importance of organs-on-a-chip in studying liver diseases in vitro, reflecting on existing reviews of healthy liver-on-a-chip platforms. Second, the techniques of cell cultures used in the microfluidic devices, including 2D, 3D, and spheroid cells, are explained. Next, the types of liver diseases (NAFLD, ALD, hepatitis infections, and drug injury) on-chip are explained for a further comprehensive overview of the design and methods of developing liver diseases in vitro. Finally, some challenges in design and existing solutions to them are reviewed MDPI 2022-03-10 /pmc/articles/PMC8950395/ /pubmed/35334720 http://dx.doi.org/10.3390/mi13030428 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Kanabekova, Perizat
Kadyrova, Adina
Kulsharova, Gulsim
Microfluidic Organ-on-a-Chip Devices for Liver Disease Modeling In Vitro
title Microfluidic Organ-on-a-Chip Devices for Liver Disease Modeling In Vitro
title_full Microfluidic Organ-on-a-Chip Devices for Liver Disease Modeling In Vitro
title_fullStr Microfluidic Organ-on-a-Chip Devices for Liver Disease Modeling In Vitro
title_full_unstemmed Microfluidic Organ-on-a-Chip Devices for Liver Disease Modeling In Vitro
title_short Microfluidic Organ-on-a-Chip Devices for Liver Disease Modeling In Vitro
title_sort microfluidic organ-on-a-chip devices for liver disease modeling in vitro
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950395/
https://www.ncbi.nlm.nih.gov/pubmed/35334720
http://dx.doi.org/10.3390/mi13030428
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