Cargando…

Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection

In light of the ever-increasing number of multidrug-resistant bacteria worldwide, bacteriophages are becoming a valid alternative to antibiotics; therefore, their interactions with host bacteria must be thoroughly investigated. Here, we report genome-wide transcriptional changes in a clinical Staphy...

Descripción completa

Detalles Bibliográficos
Autores principales: Kuptsov, Nikita, Kornienko, Maria, Bespiatykh, Dmitry, Gorodnichev, Roman, Klimina, Ksenia, Veselovsky, Vladimir, Shitikov, Egor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950790/
https://www.ncbi.nlm.nih.gov/pubmed/35336974
http://dx.doi.org/10.3390/v14030567
_version_ 1784675227552186368
author Kuptsov, Nikita
Kornienko, Maria
Bespiatykh, Dmitry
Gorodnichev, Roman
Klimina, Ksenia
Veselovsky, Vladimir
Shitikov, Egor
author_facet Kuptsov, Nikita
Kornienko, Maria
Bespiatykh, Dmitry
Gorodnichev, Roman
Klimina, Ksenia
Veselovsky, Vladimir
Shitikov, Egor
author_sort Kuptsov, Nikita
collection PubMed
description In light of the ever-increasing number of multidrug-resistant bacteria worldwide, bacteriophages are becoming a valid alternative to antibiotics; therefore, their interactions with host bacteria must be thoroughly investigated. Here, we report genome-wide transcriptional changes in a clinical Staphylococcus aureus SA515 strain for three time points after infection with the vB_SauM-515A1 kayvirus. Using an RNA sequencing approach, we identify 263 genes that were differentially expressed (DEGs) between phage-infected and uninfected host samples. Most of the DEGs were identified at an early stage of phage infection and were mainly involved in nucleotide and amino acid metabolism, as well as in cell death prevention. At the subsequent infection stages, the vast majority of DEGs were upregulated. Interestingly, 39 upregulated DEGs were common between the 15th and 30th minutes post-infection, and a substantial number of them belonged to the prophages. Furthermore, some virulence factors were overexpressed at the late infection stage, which necessitates more stringent host strain selection requirements for further use of bacteriophages for therapeutic purposes. Thus, this work allows us to better understand the influence of kayviruses on the metabolic systems of S. aureus and contributes to a better comprehension of phage therapy.
format Online
Article
Text
id pubmed-8950790
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89507902022-03-26 Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection Kuptsov, Nikita Kornienko, Maria Bespiatykh, Dmitry Gorodnichev, Roman Klimina, Ksenia Veselovsky, Vladimir Shitikov, Egor Viruses Article In light of the ever-increasing number of multidrug-resistant bacteria worldwide, bacteriophages are becoming a valid alternative to antibiotics; therefore, their interactions with host bacteria must be thoroughly investigated. Here, we report genome-wide transcriptional changes in a clinical Staphylococcus aureus SA515 strain for three time points after infection with the vB_SauM-515A1 kayvirus. Using an RNA sequencing approach, we identify 263 genes that were differentially expressed (DEGs) between phage-infected and uninfected host samples. Most of the DEGs were identified at an early stage of phage infection and were mainly involved in nucleotide and amino acid metabolism, as well as in cell death prevention. At the subsequent infection stages, the vast majority of DEGs were upregulated. Interestingly, 39 upregulated DEGs were common between the 15th and 30th minutes post-infection, and a substantial number of them belonged to the prophages. Furthermore, some virulence factors were overexpressed at the late infection stage, which necessitates more stringent host strain selection requirements for further use of bacteriophages for therapeutic purposes. Thus, this work allows us to better understand the influence of kayviruses on the metabolic systems of S. aureus and contributes to a better comprehension of phage therapy. MDPI 2022-03-09 /pmc/articles/PMC8950790/ /pubmed/35336974 http://dx.doi.org/10.3390/v14030567 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kuptsov, Nikita
Kornienko, Maria
Bespiatykh, Dmitry
Gorodnichev, Roman
Klimina, Ksenia
Veselovsky, Vladimir
Shitikov, Egor
Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection
title Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection
title_full Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection
title_fullStr Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection
title_full_unstemmed Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection
title_short Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection
title_sort global transcriptomic response of staphylococcus aureus to virulent bacteriophage infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950790/
https://www.ncbi.nlm.nih.gov/pubmed/35336974
http://dx.doi.org/10.3390/v14030567
work_keys_str_mv AT kuptsovnikita globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection
AT kornienkomaria globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection
AT bespiatykhdmitry globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection
AT gorodnichevroman globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection
AT kliminaksenia globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection
AT veselovskyvladimir globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection
AT shitikovegor globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection