Cargando…
Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection
In light of the ever-increasing number of multidrug-resistant bacteria worldwide, bacteriophages are becoming a valid alternative to antibiotics; therefore, their interactions with host bacteria must be thoroughly investigated. Here, we report genome-wide transcriptional changes in a clinical Staphy...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950790/ https://www.ncbi.nlm.nih.gov/pubmed/35336974 http://dx.doi.org/10.3390/v14030567 |
_version_ | 1784675227552186368 |
---|---|
author | Kuptsov, Nikita Kornienko, Maria Bespiatykh, Dmitry Gorodnichev, Roman Klimina, Ksenia Veselovsky, Vladimir Shitikov, Egor |
author_facet | Kuptsov, Nikita Kornienko, Maria Bespiatykh, Dmitry Gorodnichev, Roman Klimina, Ksenia Veselovsky, Vladimir Shitikov, Egor |
author_sort | Kuptsov, Nikita |
collection | PubMed |
description | In light of the ever-increasing number of multidrug-resistant bacteria worldwide, bacteriophages are becoming a valid alternative to antibiotics; therefore, their interactions with host bacteria must be thoroughly investigated. Here, we report genome-wide transcriptional changes in a clinical Staphylococcus aureus SA515 strain for three time points after infection with the vB_SauM-515A1 kayvirus. Using an RNA sequencing approach, we identify 263 genes that were differentially expressed (DEGs) between phage-infected and uninfected host samples. Most of the DEGs were identified at an early stage of phage infection and were mainly involved in nucleotide and amino acid metabolism, as well as in cell death prevention. At the subsequent infection stages, the vast majority of DEGs were upregulated. Interestingly, 39 upregulated DEGs were common between the 15th and 30th minutes post-infection, and a substantial number of them belonged to the prophages. Furthermore, some virulence factors were overexpressed at the late infection stage, which necessitates more stringent host strain selection requirements for further use of bacteriophages for therapeutic purposes. Thus, this work allows us to better understand the influence of kayviruses on the metabolic systems of S. aureus and contributes to a better comprehension of phage therapy. |
format | Online Article Text |
id | pubmed-8950790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89507902022-03-26 Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection Kuptsov, Nikita Kornienko, Maria Bespiatykh, Dmitry Gorodnichev, Roman Klimina, Ksenia Veselovsky, Vladimir Shitikov, Egor Viruses Article In light of the ever-increasing number of multidrug-resistant bacteria worldwide, bacteriophages are becoming a valid alternative to antibiotics; therefore, their interactions with host bacteria must be thoroughly investigated. Here, we report genome-wide transcriptional changes in a clinical Staphylococcus aureus SA515 strain for three time points after infection with the vB_SauM-515A1 kayvirus. Using an RNA sequencing approach, we identify 263 genes that were differentially expressed (DEGs) between phage-infected and uninfected host samples. Most of the DEGs were identified at an early stage of phage infection and were mainly involved in nucleotide and amino acid metabolism, as well as in cell death prevention. At the subsequent infection stages, the vast majority of DEGs were upregulated. Interestingly, 39 upregulated DEGs were common between the 15th and 30th minutes post-infection, and a substantial number of them belonged to the prophages. Furthermore, some virulence factors were overexpressed at the late infection stage, which necessitates more stringent host strain selection requirements for further use of bacteriophages for therapeutic purposes. Thus, this work allows us to better understand the influence of kayviruses on the metabolic systems of S. aureus and contributes to a better comprehension of phage therapy. MDPI 2022-03-09 /pmc/articles/PMC8950790/ /pubmed/35336974 http://dx.doi.org/10.3390/v14030567 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kuptsov, Nikita Kornienko, Maria Bespiatykh, Dmitry Gorodnichev, Roman Klimina, Ksenia Veselovsky, Vladimir Shitikov, Egor Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection |
title | Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection |
title_full | Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection |
title_fullStr | Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection |
title_full_unstemmed | Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection |
title_short | Global Transcriptomic Response of Staphylococcus aureus to Virulent Bacteriophage Infection |
title_sort | global transcriptomic response of staphylococcus aureus to virulent bacteriophage infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950790/ https://www.ncbi.nlm.nih.gov/pubmed/35336974 http://dx.doi.org/10.3390/v14030567 |
work_keys_str_mv | AT kuptsovnikita globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection AT kornienkomaria globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection AT bespiatykhdmitry globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection AT gorodnichevroman globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection AT kliminaksenia globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection AT veselovskyvladimir globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection AT shitikovegor globaltranscriptomicresponseofstaphylococcusaureustovirulentbacteriophageinfection |