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Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant
The global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become more serious because of the continuous emergence of variants of concern (VOC), thus calling for the development of broad-spectrum vaccines with greater efficac...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950793/ https://www.ncbi.nlm.nih.gov/pubmed/35336907 http://dx.doi.org/10.3390/v14030501 |
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author | Zhang, Naru Ji, Qianting Liu, Zezhong Tang, Kaiming Xie, Yubin Li, Kangchen Zhou, Jie Li, Sisi Shang, Haotian Shi, Zecan Zheng, Tianyu Yao, Jiawei Lu, Lu Yuan, Shuofeng Jiang, Shibo |
author_facet | Zhang, Naru Ji, Qianting Liu, Zezhong Tang, Kaiming Xie, Yubin Li, Kangchen Zhou, Jie Li, Sisi Shang, Haotian Shi, Zecan Zheng, Tianyu Yao, Jiawei Lu, Lu Yuan, Shuofeng Jiang, Shibo |
author_sort | Zhang, Naru |
collection | PubMed |
description | The global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become more serious because of the continuous emergence of variants of concern (VOC), thus calling for the development of broad-spectrum vaccines with greater efficacy. Adjuvants play important roles in enhancing the immunogenicity of protein-based subunit vaccines. In this study, we compared the effect of three adjuvants, including aluminum, nanoparticle manganese and MF59, on the immunogenicity of three protein-based COVID-19 vaccine candidates, including RBD-Fc, RBD and S-trimer. We found that the nanoparticle manganese adjuvant elicited the highest titers of SARS-CoV-2 RBD-specific IgG, IgG1 and IgG2a, as well as neutralizing antibodies against infection by pseudotyped SARS-CoV-2 and its Delta variant. What is more, the nanoparticle manganese adjuvant effectively reduced the viral load of the authentic SARS-CoV-2 and Delta variant in the cell culture supernatants. These results suggest that nanoparticle manganese, known to facilitate cGAS-STING activation, is an optimal adjuvant for protein-based COVID-19 subunit vaccines. |
format | Online Article Text |
id | pubmed-8950793 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89507932022-03-26 Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant Zhang, Naru Ji, Qianting Liu, Zezhong Tang, Kaiming Xie, Yubin Li, Kangchen Zhou, Jie Li, Sisi Shang, Haotian Shi, Zecan Zheng, Tianyu Yao, Jiawei Lu, Lu Yuan, Shuofeng Jiang, Shibo Viruses Article The global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become more serious because of the continuous emergence of variants of concern (VOC), thus calling for the development of broad-spectrum vaccines with greater efficacy. Adjuvants play important roles in enhancing the immunogenicity of protein-based subunit vaccines. In this study, we compared the effect of three adjuvants, including aluminum, nanoparticle manganese and MF59, on the immunogenicity of three protein-based COVID-19 vaccine candidates, including RBD-Fc, RBD and S-trimer. We found that the nanoparticle manganese adjuvant elicited the highest titers of SARS-CoV-2 RBD-specific IgG, IgG1 and IgG2a, as well as neutralizing antibodies against infection by pseudotyped SARS-CoV-2 and its Delta variant. What is more, the nanoparticle manganese adjuvant effectively reduced the viral load of the authentic SARS-CoV-2 and Delta variant in the cell culture supernatants. These results suggest that nanoparticle manganese, known to facilitate cGAS-STING activation, is an optimal adjuvant for protein-based COVID-19 subunit vaccines. MDPI 2022-02-28 /pmc/articles/PMC8950793/ /pubmed/35336907 http://dx.doi.org/10.3390/v14030501 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Naru Ji, Qianting Liu, Zezhong Tang, Kaiming Xie, Yubin Li, Kangchen Zhou, Jie Li, Sisi Shang, Haotian Shi, Zecan Zheng, Tianyu Yao, Jiawei Lu, Lu Yuan, Shuofeng Jiang, Shibo Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant |
title | Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant |
title_full | Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant |
title_fullStr | Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant |
title_full_unstemmed | Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant |
title_short | Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant |
title_sort | effect of different adjuvants on immune responses elicited by protein-based subunit vaccines against sars-cov-2 and its delta variant |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950793/ https://www.ncbi.nlm.nih.gov/pubmed/35336907 http://dx.doi.org/10.3390/v14030501 |
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