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Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant

The global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become more serious because of the continuous emergence of variants of concern (VOC), thus calling for the development of broad-spectrum vaccines with greater efficac...

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Autores principales: Zhang, Naru, Ji, Qianting, Liu, Zezhong, Tang, Kaiming, Xie, Yubin, Li, Kangchen, Zhou, Jie, Li, Sisi, Shang, Haotian, Shi, Zecan, Zheng, Tianyu, Yao, Jiawei, Lu, Lu, Yuan, Shuofeng, Jiang, Shibo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950793/
https://www.ncbi.nlm.nih.gov/pubmed/35336907
http://dx.doi.org/10.3390/v14030501
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author Zhang, Naru
Ji, Qianting
Liu, Zezhong
Tang, Kaiming
Xie, Yubin
Li, Kangchen
Zhou, Jie
Li, Sisi
Shang, Haotian
Shi, Zecan
Zheng, Tianyu
Yao, Jiawei
Lu, Lu
Yuan, Shuofeng
Jiang, Shibo
author_facet Zhang, Naru
Ji, Qianting
Liu, Zezhong
Tang, Kaiming
Xie, Yubin
Li, Kangchen
Zhou, Jie
Li, Sisi
Shang, Haotian
Shi, Zecan
Zheng, Tianyu
Yao, Jiawei
Lu, Lu
Yuan, Shuofeng
Jiang, Shibo
author_sort Zhang, Naru
collection PubMed
description The global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become more serious because of the continuous emergence of variants of concern (VOC), thus calling for the development of broad-spectrum vaccines with greater efficacy. Adjuvants play important roles in enhancing the immunogenicity of protein-based subunit vaccines. In this study, we compared the effect of three adjuvants, including aluminum, nanoparticle manganese and MF59, on the immunogenicity of three protein-based COVID-19 vaccine candidates, including RBD-Fc, RBD and S-trimer. We found that the nanoparticle manganese adjuvant elicited the highest titers of SARS-CoV-2 RBD-specific IgG, IgG1 and IgG2a, as well as neutralizing antibodies against infection by pseudotyped SARS-CoV-2 and its Delta variant. What is more, the nanoparticle manganese adjuvant effectively reduced the viral load of the authentic SARS-CoV-2 and Delta variant in the cell culture supernatants. These results suggest that nanoparticle manganese, known to facilitate cGAS-STING activation, is an optimal adjuvant for protein-based COVID-19 subunit vaccines.
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spelling pubmed-89507932022-03-26 Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant Zhang, Naru Ji, Qianting Liu, Zezhong Tang, Kaiming Xie, Yubin Li, Kangchen Zhou, Jie Li, Sisi Shang, Haotian Shi, Zecan Zheng, Tianyu Yao, Jiawei Lu, Lu Yuan, Shuofeng Jiang, Shibo Viruses Article The global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become more serious because of the continuous emergence of variants of concern (VOC), thus calling for the development of broad-spectrum vaccines with greater efficacy. Adjuvants play important roles in enhancing the immunogenicity of protein-based subunit vaccines. In this study, we compared the effect of three adjuvants, including aluminum, nanoparticle manganese and MF59, on the immunogenicity of three protein-based COVID-19 vaccine candidates, including RBD-Fc, RBD and S-trimer. We found that the nanoparticle manganese adjuvant elicited the highest titers of SARS-CoV-2 RBD-specific IgG, IgG1 and IgG2a, as well as neutralizing antibodies against infection by pseudotyped SARS-CoV-2 and its Delta variant. What is more, the nanoparticle manganese adjuvant effectively reduced the viral load of the authentic SARS-CoV-2 and Delta variant in the cell culture supernatants. These results suggest that nanoparticle manganese, known to facilitate cGAS-STING activation, is an optimal adjuvant for protein-based COVID-19 subunit vaccines. MDPI 2022-02-28 /pmc/articles/PMC8950793/ /pubmed/35336907 http://dx.doi.org/10.3390/v14030501 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Naru
Ji, Qianting
Liu, Zezhong
Tang, Kaiming
Xie, Yubin
Li, Kangchen
Zhou, Jie
Li, Sisi
Shang, Haotian
Shi, Zecan
Zheng, Tianyu
Yao, Jiawei
Lu, Lu
Yuan, Shuofeng
Jiang, Shibo
Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant
title Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant
title_full Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant
title_fullStr Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant
title_full_unstemmed Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant
title_short Effect of Different Adjuvants on Immune Responses Elicited by Protein-Based Subunit Vaccines against SARS-CoV-2 and Its Delta Variant
title_sort effect of different adjuvants on immune responses elicited by protein-based subunit vaccines against sars-cov-2 and its delta variant
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950793/
https://www.ncbi.nlm.nih.gov/pubmed/35336907
http://dx.doi.org/10.3390/v14030501
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