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Neuroprotective Effect of 3′,4′-Dihydroxyphenylglycol in Type-1-like Diabetic Rats—Influence of the Hydroxytyrosol/3′,4′-dihydroxyphenylglycol Ratio

The aim of this study was to assess the possible neuroprotective effect of 3′,4′-dihydroxyphenylglycol (DHPG), a polyphenol from extra virgin olive oil (EVOO), in an experimental model of diabetes and whether this effect is modified by the presence of another EVOO polyphenol, hydroxytyrosol (HT). Th...

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Autores principales: Rodríguez-Pérez, María Dolores, Pérez de Algaba, Inmaculada, Martín-Aurioles, Esther, Arrebola, María Monsalud, Ortega-Hombrados, Laura, Verdugo, Cristina, Fernández-Prior, María África, Bermúdez-Oria, Alejandra, De La Cruz, José Pedro, González-Correa, José Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950825/
https://www.ncbi.nlm.nih.gov/pubmed/35334801
http://dx.doi.org/10.3390/nu14061146
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author Rodríguez-Pérez, María Dolores
Pérez de Algaba, Inmaculada
Martín-Aurioles, Esther
Arrebola, María Monsalud
Ortega-Hombrados, Laura
Verdugo, Cristina
Fernández-Prior, María África
Bermúdez-Oria, Alejandra
De La Cruz, José Pedro
González-Correa, José Antonio
author_facet Rodríguez-Pérez, María Dolores
Pérez de Algaba, Inmaculada
Martín-Aurioles, Esther
Arrebola, María Monsalud
Ortega-Hombrados, Laura
Verdugo, Cristina
Fernández-Prior, María África
Bermúdez-Oria, Alejandra
De La Cruz, José Pedro
González-Correa, José Antonio
author_sort Rodríguez-Pérez, María Dolores
collection PubMed
description The aim of this study was to assess the possible neuroprotective effect of 3′,4′-dihydroxyphenylglycol (DHPG), a polyphenol from extra virgin olive oil (EVOO), in an experimental model of diabetes and whether this effect is modified by the presence of another EVOO polyphenol, hydroxytyrosol (HT). The neuroprotective effect was assessed in a hypoxia–reoxygenation model in brain slices and by quantifying retinal nerve cells. The animals were distributed as follows: (1) normoglycemic rats (NDR), (2) diabetic rats (DR), (3) DR treated with HT (5 mg/kg/day p.o.), (4) DR treated with DHPG (0.5 mg/kg/day), or (5) with 1 mg/kg/day, (6) DR treated with HT plus DHPG 0.5 mg/kg/day, or (7) HT plus 1 mg/kg/day p.o. DHPG. Diabetic animals presented higher levels of oxidative stress variables and lower numbers of neuronal cells in retinal tissue. The administration of DHPG or HT reduced most of the oxidative stress variables and brain lactate dehydrogenase efflux (LDH) as an indirect index of cellular death and also reduced the loss of retinal cells. The association of DHPG+HT in the same proportions, as found in EVOO, improved the neuroprotective and antioxidant effects of both polyphenols.
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spelling pubmed-89508252022-03-26 Neuroprotective Effect of 3′,4′-Dihydroxyphenylglycol in Type-1-like Diabetic Rats—Influence of the Hydroxytyrosol/3′,4′-dihydroxyphenylglycol Ratio Rodríguez-Pérez, María Dolores Pérez de Algaba, Inmaculada Martín-Aurioles, Esther Arrebola, María Monsalud Ortega-Hombrados, Laura Verdugo, Cristina Fernández-Prior, María África Bermúdez-Oria, Alejandra De La Cruz, José Pedro González-Correa, José Antonio Nutrients Article The aim of this study was to assess the possible neuroprotective effect of 3′,4′-dihydroxyphenylglycol (DHPG), a polyphenol from extra virgin olive oil (EVOO), in an experimental model of diabetes and whether this effect is modified by the presence of another EVOO polyphenol, hydroxytyrosol (HT). The neuroprotective effect was assessed in a hypoxia–reoxygenation model in brain slices and by quantifying retinal nerve cells. The animals were distributed as follows: (1) normoglycemic rats (NDR), (2) diabetic rats (DR), (3) DR treated with HT (5 mg/kg/day p.o.), (4) DR treated with DHPG (0.5 mg/kg/day), or (5) with 1 mg/kg/day, (6) DR treated with HT plus DHPG 0.5 mg/kg/day, or (7) HT plus 1 mg/kg/day p.o. DHPG. Diabetic animals presented higher levels of oxidative stress variables and lower numbers of neuronal cells in retinal tissue. The administration of DHPG or HT reduced most of the oxidative stress variables and brain lactate dehydrogenase efflux (LDH) as an indirect index of cellular death and also reduced the loss of retinal cells. The association of DHPG+HT in the same proportions, as found in EVOO, improved the neuroprotective and antioxidant effects of both polyphenols. MDPI 2022-03-08 /pmc/articles/PMC8950825/ /pubmed/35334801 http://dx.doi.org/10.3390/nu14061146 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodríguez-Pérez, María Dolores
Pérez de Algaba, Inmaculada
Martín-Aurioles, Esther
Arrebola, María Monsalud
Ortega-Hombrados, Laura
Verdugo, Cristina
Fernández-Prior, María África
Bermúdez-Oria, Alejandra
De La Cruz, José Pedro
González-Correa, José Antonio
Neuroprotective Effect of 3′,4′-Dihydroxyphenylglycol in Type-1-like Diabetic Rats—Influence of the Hydroxytyrosol/3′,4′-dihydroxyphenylglycol Ratio
title Neuroprotective Effect of 3′,4′-Dihydroxyphenylglycol in Type-1-like Diabetic Rats—Influence of the Hydroxytyrosol/3′,4′-dihydroxyphenylglycol Ratio
title_full Neuroprotective Effect of 3′,4′-Dihydroxyphenylglycol in Type-1-like Diabetic Rats—Influence of the Hydroxytyrosol/3′,4′-dihydroxyphenylglycol Ratio
title_fullStr Neuroprotective Effect of 3′,4′-Dihydroxyphenylglycol in Type-1-like Diabetic Rats—Influence of the Hydroxytyrosol/3′,4′-dihydroxyphenylglycol Ratio
title_full_unstemmed Neuroprotective Effect of 3′,4′-Dihydroxyphenylglycol in Type-1-like Diabetic Rats—Influence of the Hydroxytyrosol/3′,4′-dihydroxyphenylglycol Ratio
title_short Neuroprotective Effect of 3′,4′-Dihydroxyphenylglycol in Type-1-like Diabetic Rats—Influence of the Hydroxytyrosol/3′,4′-dihydroxyphenylglycol Ratio
title_sort neuroprotective effect of 3′,4′-dihydroxyphenylglycol in type-1-like diabetic rats—influence of the hydroxytyrosol/3′,4′-dihydroxyphenylglycol ratio
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950825/
https://www.ncbi.nlm.nih.gov/pubmed/35334801
http://dx.doi.org/10.3390/nu14061146
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