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Laser Direct Writing of Dual-Scale 3D Structures for Cell Repelling at High Cellular Density

The fabrication of complex, reproducible, and accurate micro-and nanostructured interfaces that impede the interaction between material’s surface and different cell types represents an important objective in the development of medical devices. This can be achieved by topographical means such as dual...

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Detalles Bibliográficos
Autores principales: Paun, Irina Alexandra, Calin, Bogdan Stefanita, Popescu, Roxana Cristina, Tanasa, Eugenia, Moldovan, Antoniu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950975/
https://www.ncbi.nlm.nih.gov/pubmed/35328668
http://dx.doi.org/10.3390/ijms23063247
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author Paun, Irina Alexandra
Calin, Bogdan Stefanita
Popescu, Roxana Cristina
Tanasa, Eugenia
Moldovan, Antoniu
author_facet Paun, Irina Alexandra
Calin, Bogdan Stefanita
Popescu, Roxana Cristina
Tanasa, Eugenia
Moldovan, Antoniu
author_sort Paun, Irina Alexandra
collection PubMed
description The fabrication of complex, reproducible, and accurate micro-and nanostructured interfaces that impede the interaction between material’s surface and different cell types represents an important objective in the development of medical devices. This can be achieved by topographical means such as dual-scale structures, mainly represented by microstructures with surface nanopatterning. Fabrication via laser irradiation of materials seems promising. However, laser-assisted fabrication of dual-scale structures, i.e., ripples relies on stochastic processes deriving from laser–matter interaction, limiting the control over the structures’ topography. In this paper, we report on laser fabrication of cell-repellent dual-scale 3D structures with fully reproducible and high spatial accuracy topographies. Structures were designed as micrometric “mushrooms” decorated with fingerprint-like nanometric features with heights and periodicities close to those of the calamistrum, i.e., 200–300 nm. They were fabricated by Laser Direct Writing via Two-Photon Polymerization of IP-Dip photoresist. Design and laser writing parameters were optimized for conferring cell-repellent properties to the structures, even for high cellular densities in the culture medium. The structures were most efficient in repelling the cells when the fingerprint-like features had periodicities and heights of ≅200 nm, fairly close to the repellent surfaces of the calamistrum. Laser power was the most important parameter for the optimization protocol.
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spelling pubmed-89509752022-03-26 Laser Direct Writing of Dual-Scale 3D Structures for Cell Repelling at High Cellular Density Paun, Irina Alexandra Calin, Bogdan Stefanita Popescu, Roxana Cristina Tanasa, Eugenia Moldovan, Antoniu Int J Mol Sci Article The fabrication of complex, reproducible, and accurate micro-and nanostructured interfaces that impede the interaction between material’s surface and different cell types represents an important objective in the development of medical devices. This can be achieved by topographical means such as dual-scale structures, mainly represented by microstructures with surface nanopatterning. Fabrication via laser irradiation of materials seems promising. However, laser-assisted fabrication of dual-scale structures, i.e., ripples relies on stochastic processes deriving from laser–matter interaction, limiting the control over the structures’ topography. In this paper, we report on laser fabrication of cell-repellent dual-scale 3D structures with fully reproducible and high spatial accuracy topographies. Structures were designed as micrometric “mushrooms” decorated with fingerprint-like nanometric features with heights and periodicities close to those of the calamistrum, i.e., 200–300 nm. They were fabricated by Laser Direct Writing via Two-Photon Polymerization of IP-Dip photoresist. Design and laser writing parameters were optimized for conferring cell-repellent properties to the structures, even for high cellular densities in the culture medium. The structures were most efficient in repelling the cells when the fingerprint-like features had periodicities and heights of ≅200 nm, fairly close to the repellent surfaces of the calamistrum. Laser power was the most important parameter for the optimization protocol. MDPI 2022-03-17 /pmc/articles/PMC8950975/ /pubmed/35328668 http://dx.doi.org/10.3390/ijms23063247 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Paun, Irina Alexandra
Calin, Bogdan Stefanita
Popescu, Roxana Cristina
Tanasa, Eugenia
Moldovan, Antoniu
Laser Direct Writing of Dual-Scale 3D Structures for Cell Repelling at High Cellular Density
title Laser Direct Writing of Dual-Scale 3D Structures for Cell Repelling at High Cellular Density
title_full Laser Direct Writing of Dual-Scale 3D Structures for Cell Repelling at High Cellular Density
title_fullStr Laser Direct Writing of Dual-Scale 3D Structures for Cell Repelling at High Cellular Density
title_full_unstemmed Laser Direct Writing of Dual-Scale 3D Structures for Cell Repelling at High Cellular Density
title_short Laser Direct Writing of Dual-Scale 3D Structures for Cell Repelling at High Cellular Density
title_sort laser direct writing of dual-scale 3d structures for cell repelling at high cellular density
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950975/
https://www.ncbi.nlm.nih.gov/pubmed/35328668
http://dx.doi.org/10.3390/ijms23063247
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