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Deterministic Lateral Displacement Microfluidic Chip for Minicell Purification

Deterministic lateral displacement (DLD) is a well-known microfluidic technique for particle separation with high potential for integration into bioreactors for therapeutic applications. Separation is based on the interaction of suspended particles in a liquid flowing through an array of microposts...

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Autores principales: Sherbaz, Ahmad, Konak, Büşra M. K., Pezeshkpour, Pegah, Di Ventura, Barbara, Rapp, Bastian E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951003/
https://www.ncbi.nlm.nih.gov/pubmed/35334657
http://dx.doi.org/10.3390/mi13030365
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author Sherbaz, Ahmad
Konak, Büşra M. K.
Pezeshkpour, Pegah
Di Ventura, Barbara
Rapp, Bastian E.
author_facet Sherbaz, Ahmad
Konak, Büşra M. K.
Pezeshkpour, Pegah
Di Ventura, Barbara
Rapp, Bastian E.
author_sort Sherbaz, Ahmad
collection PubMed
description Deterministic lateral displacement (DLD) is a well-known microfluidic technique for particle separation with high potential for integration into bioreactors for therapeutic applications. Separation is based on the interaction of suspended particles in a liquid flowing through an array of microposts under low Reynolds conditions. This technique has been used previously to separate living cells of different sizes but similar shapes. Here, we present a DLD microchip to separate rod-shaped bacterial cells up to 10 µm from submicron spherical minicells. We designed two microchips with 50 and 25 µm cylindrical posts and spacing of 15 and 2.5 µm, respectively. Soft lithography was used to fabricate polydimethylsiloxane (PDMS) chips, which were assessed at different flow rates for their separation potential. The results showed negligible shear effect on the separation efficiency for both designs. However, the higher flow rates resulted in faster separation. We optimized the geometrical parameters including the shape, size, angle and critical radii of the posts and the width and depth of the channel as well as the number of arrays to achieve separation efficiency as high as 75.5% on a single-stage separation. These results pave the way for high-throughput separation and purification modules with the potential of direct integration into bioreactors.
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spelling pubmed-89510032022-03-26 Deterministic Lateral Displacement Microfluidic Chip for Minicell Purification Sherbaz, Ahmad Konak, Büşra M. K. Pezeshkpour, Pegah Di Ventura, Barbara Rapp, Bastian E. Micromachines (Basel) Article Deterministic lateral displacement (DLD) is a well-known microfluidic technique for particle separation with high potential for integration into bioreactors for therapeutic applications. Separation is based on the interaction of suspended particles in a liquid flowing through an array of microposts under low Reynolds conditions. This technique has been used previously to separate living cells of different sizes but similar shapes. Here, we present a DLD microchip to separate rod-shaped bacterial cells up to 10 µm from submicron spherical minicells. We designed two microchips with 50 and 25 µm cylindrical posts and spacing of 15 and 2.5 µm, respectively. Soft lithography was used to fabricate polydimethylsiloxane (PDMS) chips, which were assessed at different flow rates for their separation potential. The results showed negligible shear effect on the separation efficiency for both designs. However, the higher flow rates resulted in faster separation. We optimized the geometrical parameters including the shape, size, angle and critical radii of the posts and the width and depth of the channel as well as the number of arrays to achieve separation efficiency as high as 75.5% on a single-stage separation. These results pave the way for high-throughput separation and purification modules with the potential of direct integration into bioreactors. MDPI 2022-02-25 /pmc/articles/PMC8951003/ /pubmed/35334657 http://dx.doi.org/10.3390/mi13030365 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sherbaz, Ahmad
Konak, Büşra M. K.
Pezeshkpour, Pegah
Di Ventura, Barbara
Rapp, Bastian E.
Deterministic Lateral Displacement Microfluidic Chip for Minicell Purification
title Deterministic Lateral Displacement Microfluidic Chip for Minicell Purification
title_full Deterministic Lateral Displacement Microfluidic Chip for Minicell Purification
title_fullStr Deterministic Lateral Displacement Microfluidic Chip for Minicell Purification
title_full_unstemmed Deterministic Lateral Displacement Microfluidic Chip for Minicell Purification
title_short Deterministic Lateral Displacement Microfluidic Chip for Minicell Purification
title_sort deterministic lateral displacement microfluidic chip for minicell purification
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951003/
https://www.ncbi.nlm.nih.gov/pubmed/35334657
http://dx.doi.org/10.3390/mi13030365
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