Cargando…

KASP Markers Specific for the Fertility Restorer Locus Rf1 and Application for Genetic Purity Testing in Sunflowers (Helianthus annuus L.)

Single nucleotide polymorphisms (SNPs) were significantly associated with fertility restoration of cytoplasmic male sterility (CMS) PET1 by the restorer gene Rf1. For these SNPs, four Kompetitive allele-specific PCR (KASP) markers were successfully designed. The KASP markers cover the fertility rest...

Descripción completa

Detalles Bibliográficos
Autores principales: Radanović, Aleksandra, Sprycha, Yves, Jocković, Milan, Sundt, Monja, Miladinović, Dragana, Jansen, Constantin, Horn, Renate
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951052/
https://www.ncbi.nlm.nih.gov/pubmed/35328019
http://dx.doi.org/10.3390/genes13030465
_version_ 1784675291063386112
author Radanović, Aleksandra
Sprycha, Yves
Jocković, Milan
Sundt, Monja
Miladinović, Dragana
Jansen, Constantin
Horn, Renate
author_facet Radanović, Aleksandra
Sprycha, Yves
Jocković, Milan
Sundt, Monja
Miladinović, Dragana
Jansen, Constantin
Horn, Renate
author_sort Radanović, Aleksandra
collection PubMed
description Single nucleotide polymorphisms (SNPs) were significantly associated with fertility restoration of cytoplasmic male sterility (CMS) PET1 by the restorer gene Rf1. For these SNPs, four Kompetitive allele-specific PCR (KASP) markers were successfully designed. The KASP markers cover the fertility restorer locus Rf1, spanning about 3 Mb, and clearly differentiate restorer and maintainer lines. For genetic purity testing in sunflower hybrid production, the efficiency for detecting contaminations in samples was simulated using mixtures of hypocotyls or leaves. Contaminations of restorer lines with 1%, 3%, 5%, 10%, and 50% of maintainer lines were screened with all four KASP markers. Contaminations of 10% could be clearly detected in pools of 100 plants. Contaminations below this level require detection on a single plant level. For single plant detections, ethyl methanesulfonate-treated sunflower F1 hybrids, which had been phenotypically evaluated for male sterility (potential mutation in the Rf1 gene) were screened. Nine identified either partially male-sterile or male-sterile plants were analyzed with all four KASP markers and only one proved to be a hybrid with a mutation, seven were male-sterile contaminants in the F1 seeds used (1.6%) and one a recombinant plant. The four KASP markers should be valuable tools for marker-assisted selection (MAS) in sunflower breeding regarding the restorer locus Rf1.
format Online
Article
Text
id pubmed-8951052
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89510522022-03-26 KASP Markers Specific for the Fertility Restorer Locus Rf1 and Application for Genetic Purity Testing in Sunflowers (Helianthus annuus L.) Radanović, Aleksandra Sprycha, Yves Jocković, Milan Sundt, Monja Miladinović, Dragana Jansen, Constantin Horn, Renate Genes (Basel) Article Single nucleotide polymorphisms (SNPs) were significantly associated with fertility restoration of cytoplasmic male sterility (CMS) PET1 by the restorer gene Rf1. For these SNPs, four Kompetitive allele-specific PCR (KASP) markers were successfully designed. The KASP markers cover the fertility restorer locus Rf1, spanning about 3 Mb, and clearly differentiate restorer and maintainer lines. For genetic purity testing in sunflower hybrid production, the efficiency for detecting contaminations in samples was simulated using mixtures of hypocotyls or leaves. Contaminations of restorer lines with 1%, 3%, 5%, 10%, and 50% of maintainer lines were screened with all four KASP markers. Contaminations of 10% could be clearly detected in pools of 100 plants. Contaminations below this level require detection on a single plant level. For single plant detections, ethyl methanesulfonate-treated sunflower F1 hybrids, which had been phenotypically evaluated for male sterility (potential mutation in the Rf1 gene) were screened. Nine identified either partially male-sterile or male-sterile plants were analyzed with all four KASP markers and only one proved to be a hybrid with a mutation, seven were male-sterile contaminants in the F1 seeds used (1.6%) and one a recombinant plant. The four KASP markers should be valuable tools for marker-assisted selection (MAS) in sunflower breeding regarding the restorer locus Rf1. MDPI 2022-03-05 /pmc/articles/PMC8951052/ /pubmed/35328019 http://dx.doi.org/10.3390/genes13030465 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Radanović, Aleksandra
Sprycha, Yves
Jocković, Milan
Sundt, Monja
Miladinović, Dragana
Jansen, Constantin
Horn, Renate
KASP Markers Specific for the Fertility Restorer Locus Rf1 and Application for Genetic Purity Testing in Sunflowers (Helianthus annuus L.)
title KASP Markers Specific for the Fertility Restorer Locus Rf1 and Application for Genetic Purity Testing in Sunflowers (Helianthus annuus L.)
title_full KASP Markers Specific for the Fertility Restorer Locus Rf1 and Application for Genetic Purity Testing in Sunflowers (Helianthus annuus L.)
title_fullStr KASP Markers Specific for the Fertility Restorer Locus Rf1 and Application for Genetic Purity Testing in Sunflowers (Helianthus annuus L.)
title_full_unstemmed KASP Markers Specific for the Fertility Restorer Locus Rf1 and Application for Genetic Purity Testing in Sunflowers (Helianthus annuus L.)
title_short KASP Markers Specific for the Fertility Restorer Locus Rf1 and Application for Genetic Purity Testing in Sunflowers (Helianthus annuus L.)
title_sort kasp markers specific for the fertility restorer locus rf1 and application for genetic purity testing in sunflowers (helianthus annuus l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951052/
https://www.ncbi.nlm.nih.gov/pubmed/35328019
http://dx.doi.org/10.3390/genes13030465
work_keys_str_mv AT radanovicaleksandra kaspmarkersspecificforthefertilityrestorerlocusrf1andapplicationforgeneticpuritytestinginsunflowershelianthusannuusl
AT sprychayves kaspmarkersspecificforthefertilityrestorerlocusrf1andapplicationforgeneticpuritytestinginsunflowershelianthusannuusl
AT jockovicmilan kaspmarkersspecificforthefertilityrestorerlocusrf1andapplicationforgeneticpuritytestinginsunflowershelianthusannuusl
AT sundtmonja kaspmarkersspecificforthefertilityrestorerlocusrf1andapplicationforgeneticpuritytestinginsunflowershelianthusannuusl
AT miladinovicdragana kaspmarkersspecificforthefertilityrestorerlocusrf1andapplicationforgeneticpuritytestinginsunflowershelianthusannuusl
AT jansenconstantin kaspmarkersspecificforthefertilityrestorerlocusrf1andapplicationforgeneticpuritytestinginsunflowershelianthusannuusl
AT hornrenate kaspmarkersspecificforthefertilityrestorerlocusrf1andapplicationforgeneticpuritytestinginsunflowershelianthusannuusl