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Nanoparticles Loaded Thermoresponsive In Situ Gel for Ocular Antibiotic Delivery against Bacterial Keratitis
Antibiotics delivered through conventional dosage against ophthalmic infections show lower therapeutic efficacy due to their low residence time. Therefore, there is a great need to design and develop novel dosage forms that would increase the ocular residence time of antibiotics at the site of infec...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951139/ https://www.ncbi.nlm.nih.gov/pubmed/35335465 http://dx.doi.org/10.3390/polym14061135 |
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author | Abbas, Muhammad Naseer Khan, Saeed Ahmad Sadozai, Sajid Khan Khalil, Islam A. Anter, Asem Fouly, Marwa El Osman, Ahmed H. Kazi, Mohsin |
author_facet | Abbas, Muhammad Naseer Khan, Saeed Ahmad Sadozai, Sajid Khan Khalil, Islam A. Anter, Asem Fouly, Marwa El Osman, Ahmed H. Kazi, Mohsin |
author_sort | Abbas, Muhammad Naseer |
collection | PubMed |
description | Antibiotics delivered through conventional dosage against ophthalmic infections show lower therapeutic efficacy due to their low residence time. Therefore, there is a great need to design and develop novel dosage forms that would increase the ocular residence time of antibiotics at the site of infection. This study describes the development of nanoparticles laden in situ gelling solution, intended to sustain antibiotic release for improved therapeutic efficiency. Oxytetracycline-loaded gelatin-polyacrylic acid nanoparticles were prepared and incorporated in poloxamer-N407 solution. The rheological properties of the system were studied concerning time and temperature. Moreover, in vivo biocompatibility of the system was ascertained using the Draize test and histological studies. Finally, the optimized formulation was evaluated for in vitro antibacterial activity against one of the most common keratitis causing bacteria, Pseudomonas aeruginosa. Additionally, the in vivo efficacy was evaluated on the rabbit’s eye conjunctivitis model. The formulation showed a sustained effect against keratitis; furthermore, the antibacterial activity was comparable with the commercial product. |
format | Online Article Text |
id | pubmed-8951139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89511392022-03-26 Nanoparticles Loaded Thermoresponsive In Situ Gel for Ocular Antibiotic Delivery against Bacterial Keratitis Abbas, Muhammad Naseer Khan, Saeed Ahmad Sadozai, Sajid Khan Khalil, Islam A. Anter, Asem Fouly, Marwa El Osman, Ahmed H. Kazi, Mohsin Polymers (Basel) Article Antibiotics delivered through conventional dosage against ophthalmic infections show lower therapeutic efficacy due to their low residence time. Therefore, there is a great need to design and develop novel dosage forms that would increase the ocular residence time of antibiotics at the site of infection. This study describes the development of nanoparticles laden in situ gelling solution, intended to sustain antibiotic release for improved therapeutic efficiency. Oxytetracycline-loaded gelatin-polyacrylic acid nanoparticles were prepared and incorporated in poloxamer-N407 solution. The rheological properties of the system were studied concerning time and temperature. Moreover, in vivo biocompatibility of the system was ascertained using the Draize test and histological studies. Finally, the optimized formulation was evaluated for in vitro antibacterial activity against one of the most common keratitis causing bacteria, Pseudomonas aeruginosa. Additionally, the in vivo efficacy was evaluated on the rabbit’s eye conjunctivitis model. The formulation showed a sustained effect against keratitis; furthermore, the antibacterial activity was comparable with the commercial product. MDPI 2022-03-11 /pmc/articles/PMC8951139/ /pubmed/35335465 http://dx.doi.org/10.3390/polym14061135 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Abbas, Muhammad Naseer Khan, Saeed Ahmad Sadozai, Sajid Khan Khalil, Islam A. Anter, Asem Fouly, Marwa El Osman, Ahmed H. Kazi, Mohsin Nanoparticles Loaded Thermoresponsive In Situ Gel for Ocular Antibiotic Delivery against Bacterial Keratitis |
title | Nanoparticles Loaded Thermoresponsive In Situ Gel for Ocular Antibiotic Delivery against Bacterial Keratitis |
title_full | Nanoparticles Loaded Thermoresponsive In Situ Gel for Ocular Antibiotic Delivery against Bacterial Keratitis |
title_fullStr | Nanoparticles Loaded Thermoresponsive In Situ Gel for Ocular Antibiotic Delivery against Bacterial Keratitis |
title_full_unstemmed | Nanoparticles Loaded Thermoresponsive In Situ Gel for Ocular Antibiotic Delivery against Bacterial Keratitis |
title_short | Nanoparticles Loaded Thermoresponsive In Situ Gel for Ocular Antibiotic Delivery against Bacterial Keratitis |
title_sort | nanoparticles loaded thermoresponsive in situ gel for ocular antibiotic delivery against bacterial keratitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951139/ https://www.ncbi.nlm.nih.gov/pubmed/35335465 http://dx.doi.org/10.3390/polym14061135 |
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