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Metabolomics Mechanism and Lignin Response to Laxogenin C, a Natural Regulator of Plants Growth

Laxogenin C (LGC) is a natural spirostanol deriving from plant hormone which has shown growing regulation similar to those of brassinosteroids. In the present study, LGC showed a promoting effect on tomato seed germination and seedling growth in a dose-dependent manner. We applied LC-MS/MS to invest...

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Autores principales: Deng, Yuchan, Wang, Jiaqi, Zhang, Annan, Zhu, Zhaoju, Ren, Sipei, Zhang, Cunli, Zhang, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951225/
https://www.ncbi.nlm.nih.gov/pubmed/35328410
http://dx.doi.org/10.3390/ijms23062990
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author Deng, Yuchan
Wang, Jiaqi
Zhang, Annan
Zhu, Zhaoju
Ren, Sipei
Zhang, Cunli
Zhang, Qiang
author_facet Deng, Yuchan
Wang, Jiaqi
Zhang, Annan
Zhu, Zhaoju
Ren, Sipei
Zhang, Cunli
Zhang, Qiang
author_sort Deng, Yuchan
collection PubMed
description Laxogenin C (LGC) is a natural spirostanol deriving from plant hormone which has shown growing regulation similar to those of brassinosteroids. In the present study, LGC showed a promoting effect on tomato seed germination and seedling growth in a dose-dependent manner. We applied LC-MS/MS to investigate metabolome variations in the tomato treated with LGC, which revealed 10 differential metabolites (DMs) related to KEGG metabolites, associated with low and high doses of LGC. Enrichment and pathway mapping based on the KEGG database indicated that LGC regulated expressions of 2-hydroxycinnamic acid and l-phenylalanine to interfere with phenylalanine metabolism and phenylpropanoids biosynthesis. The two pathways are closely related to plant growth and lignin formation. In our further phenotypic verification, LGC was confirmed to affect seedling lignification and related phenylpropanoids, trans-ferulic acid and l-phenylalanine levels. These findings provided a metabolomic aspect on the plant hormone derivates and revealed the affected metabolites. Elucidating their regulation mechanisms can contribute to the development of sustainable agriculture. Further studies on agrichemical development would provide eco-friendly and efficient regulators for plant growth control and quality improvement.
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spelling pubmed-89512252022-03-26 Metabolomics Mechanism and Lignin Response to Laxogenin C, a Natural Regulator of Plants Growth Deng, Yuchan Wang, Jiaqi Zhang, Annan Zhu, Zhaoju Ren, Sipei Zhang, Cunli Zhang, Qiang Int J Mol Sci Article Laxogenin C (LGC) is a natural spirostanol deriving from plant hormone which has shown growing regulation similar to those of brassinosteroids. In the present study, LGC showed a promoting effect on tomato seed germination and seedling growth in a dose-dependent manner. We applied LC-MS/MS to investigate metabolome variations in the tomato treated with LGC, which revealed 10 differential metabolites (DMs) related to KEGG metabolites, associated with low and high doses of LGC. Enrichment and pathway mapping based on the KEGG database indicated that LGC regulated expressions of 2-hydroxycinnamic acid and l-phenylalanine to interfere with phenylalanine metabolism and phenylpropanoids biosynthesis. The two pathways are closely related to plant growth and lignin formation. In our further phenotypic verification, LGC was confirmed to affect seedling lignification and related phenylpropanoids, trans-ferulic acid and l-phenylalanine levels. These findings provided a metabolomic aspect on the plant hormone derivates and revealed the affected metabolites. Elucidating their regulation mechanisms can contribute to the development of sustainable agriculture. Further studies on agrichemical development would provide eco-friendly and efficient regulators for plant growth control and quality improvement. MDPI 2022-03-10 /pmc/articles/PMC8951225/ /pubmed/35328410 http://dx.doi.org/10.3390/ijms23062990 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Deng, Yuchan
Wang, Jiaqi
Zhang, Annan
Zhu, Zhaoju
Ren, Sipei
Zhang, Cunli
Zhang, Qiang
Metabolomics Mechanism and Lignin Response to Laxogenin C, a Natural Regulator of Plants Growth
title Metabolomics Mechanism and Lignin Response to Laxogenin C, a Natural Regulator of Plants Growth
title_full Metabolomics Mechanism and Lignin Response to Laxogenin C, a Natural Regulator of Plants Growth
title_fullStr Metabolomics Mechanism and Lignin Response to Laxogenin C, a Natural Regulator of Plants Growth
title_full_unstemmed Metabolomics Mechanism and Lignin Response to Laxogenin C, a Natural Regulator of Plants Growth
title_short Metabolomics Mechanism and Lignin Response to Laxogenin C, a Natural Regulator of Plants Growth
title_sort metabolomics mechanism and lignin response to laxogenin c, a natural regulator of plants growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951225/
https://www.ncbi.nlm.nih.gov/pubmed/35328410
http://dx.doi.org/10.3390/ijms23062990
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