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Enteric Viruses Nucleic Acids Distribution along the Digestive Tract of Rhesus Macaques with Idiopathic Chronic Diarrhea

Idiopathic chronic diarrhea (ICD) is a little understood common clinical problem in captive rhesus macaques claiming 33% of medical culls unrelated to research. The eukaryotic virome in digestive tract tissues collected at necropsy from nine animals with ICD was characterized using viral metagenomic...

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Autores principales: Delwart, Eric, Merriam, David, Ardeshir, Amir, Altan, Eda, Li, Yanpeng, Deng, Xutao, Hartigan-O’Connor, Dennis J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951234/
https://www.ncbi.nlm.nih.gov/pubmed/35337045
http://dx.doi.org/10.3390/v14030638
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author Delwart, Eric
Merriam, David
Ardeshir, Amir
Altan, Eda
Li, Yanpeng
Deng, Xutao
Hartigan-O’Connor, Dennis J.
author_facet Delwart, Eric
Merriam, David
Ardeshir, Amir
Altan, Eda
Li, Yanpeng
Deng, Xutao
Hartigan-O’Connor, Dennis J.
author_sort Delwart, Eric
collection PubMed
description Idiopathic chronic diarrhea (ICD) is a little understood common clinical problem in captive rhesus macaques claiming 33% of medical culls unrelated to research. The eukaryotic virome in digestive tract tissues collected at necropsy from nine animals with ICD was characterized using viral metagenomics. We compared the distribution of viral reads in tissues and mucosal scrapings from the stomach, duodenum, jejunum, ileum, and the proximal, transverse, and distal colons. In situ hybridization (ISH) using viral probes were performed on fixed tissues. Deep sequencing revealed multiple viruses in the Parvoviridae and Picornaviridae family. Tissues and mucosal scraping from the same locations showed closely related viral reads contents while different gut tissues from the same animal varied widely. ISH showed punctuated staining for both RNA and DNA viruses in the distal colon. Parvovirus staining was also detected in the stomach/duodenum/jejunum in distinct oval-shaped structures. The location of enteric viral nucleic acid differed widely between different viral families and along the length of the digestive tract.
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spelling pubmed-89512342022-03-26 Enteric Viruses Nucleic Acids Distribution along the Digestive Tract of Rhesus Macaques with Idiopathic Chronic Diarrhea Delwart, Eric Merriam, David Ardeshir, Amir Altan, Eda Li, Yanpeng Deng, Xutao Hartigan-O’Connor, Dennis J. Viruses Article Idiopathic chronic diarrhea (ICD) is a little understood common clinical problem in captive rhesus macaques claiming 33% of medical culls unrelated to research. The eukaryotic virome in digestive tract tissues collected at necropsy from nine animals with ICD was characterized using viral metagenomics. We compared the distribution of viral reads in tissues and mucosal scrapings from the stomach, duodenum, jejunum, ileum, and the proximal, transverse, and distal colons. In situ hybridization (ISH) using viral probes were performed on fixed tissues. Deep sequencing revealed multiple viruses in the Parvoviridae and Picornaviridae family. Tissues and mucosal scraping from the same locations showed closely related viral reads contents while different gut tissues from the same animal varied widely. ISH showed punctuated staining for both RNA and DNA viruses in the distal colon. Parvovirus staining was also detected in the stomach/duodenum/jejunum in distinct oval-shaped structures. The location of enteric viral nucleic acid differed widely between different viral families and along the length of the digestive tract. MDPI 2022-03-19 /pmc/articles/PMC8951234/ /pubmed/35337045 http://dx.doi.org/10.3390/v14030638 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Delwart, Eric
Merriam, David
Ardeshir, Amir
Altan, Eda
Li, Yanpeng
Deng, Xutao
Hartigan-O’Connor, Dennis J.
Enteric Viruses Nucleic Acids Distribution along the Digestive Tract of Rhesus Macaques with Idiopathic Chronic Diarrhea
title Enteric Viruses Nucleic Acids Distribution along the Digestive Tract of Rhesus Macaques with Idiopathic Chronic Diarrhea
title_full Enteric Viruses Nucleic Acids Distribution along the Digestive Tract of Rhesus Macaques with Idiopathic Chronic Diarrhea
title_fullStr Enteric Viruses Nucleic Acids Distribution along the Digestive Tract of Rhesus Macaques with Idiopathic Chronic Diarrhea
title_full_unstemmed Enteric Viruses Nucleic Acids Distribution along the Digestive Tract of Rhesus Macaques with Idiopathic Chronic Diarrhea
title_short Enteric Viruses Nucleic Acids Distribution along the Digestive Tract of Rhesus Macaques with Idiopathic Chronic Diarrhea
title_sort enteric viruses nucleic acids distribution along the digestive tract of rhesus macaques with idiopathic chronic diarrhea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951234/
https://www.ncbi.nlm.nih.gov/pubmed/35337045
http://dx.doi.org/10.3390/v14030638
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