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The Role of Plasma Extracellular Vesicles in Remote Ischemic Conditioning and Exercise-Induced Ischemic Tolerance
Ischemic conditioning and exercise have been suggested for protecting against brain ischemia-reperfusion injury. However, the endogenous protective mechanisms stimulated by these interventions remain unclear. Here, in a comprehensive translational study, we investigated the protective role of extrac...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951333/ https://www.ncbi.nlm.nih.gov/pubmed/35328755 http://dx.doi.org/10.3390/ijms23063334 |
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| author | Gu, Tingting Just, Jesper Stenz, Katrine Tang Yan, Yan Sieljacks, Peter Wang, Jakob Groennebaek, Thomas Skjaerlund Jakobsgaard, Jesper Emil Rindom, Emil Herskind, Jon Gravholt, Anders Lassen, Thomas Ravn Jørgensen, Mathias Bæk, Rikke Gutiérrez-Jiménez, Eugenio Iversen, Nina Kerting Rasmussen, Peter Mondrup Nyengaard, Jens Randel Jørgensen, Malene Møller de Paoli, Frank Bøtker, Hans Erik Kjems, Jørgen Vissing, Kristian Drasbek, Kim Ryun |
| author_facet | Gu, Tingting Just, Jesper Stenz, Katrine Tang Yan, Yan Sieljacks, Peter Wang, Jakob Groennebaek, Thomas Skjaerlund Jakobsgaard, Jesper Emil Rindom, Emil Herskind, Jon Gravholt, Anders Lassen, Thomas Ravn Jørgensen, Mathias Bæk, Rikke Gutiérrez-Jiménez, Eugenio Iversen, Nina Kerting Rasmussen, Peter Mondrup Nyengaard, Jens Randel Jørgensen, Malene Møller de Paoli, Frank Bøtker, Hans Erik Kjems, Jørgen Vissing, Kristian Drasbek, Kim Ryun |
| author_sort | Gu, Tingting |
| collection | PubMed |
| description | Ischemic conditioning and exercise have been suggested for protecting against brain ischemia-reperfusion injury. However, the endogenous protective mechanisms stimulated by these interventions remain unclear. Here, in a comprehensive translational study, we investigated the protective role of extracellular vesicles (EVs) released after remote ischemic conditioning (RIC), blood flow restricted resistance exercise (BFRRE), or high-load resistance exercise (HLRE). Blood samples were collected from human participants before and at serial time points after intervention. RIC and BFRRE plasma EVs released early after stimulation improved viability of endothelial cells subjected to oxygen-glucose deprivation. Furthermore, post-RIC EVs accumulated in the ischemic area of a stroke mouse model, and a mean decrease in infarct volume was observed for post-RIC EVs, although not reaching statistical significance. Thus, circulating EVs induced by RIC and BFRRE can mediate protection, but the in vivo and translational effects of conditioned EVs require further experimental verification. |
| format | Online Article Text |
| id | pubmed-8951333 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | MDPI |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-89513332022-03-26 The Role of Plasma Extracellular Vesicles in Remote Ischemic Conditioning and Exercise-Induced Ischemic Tolerance Gu, Tingting Just, Jesper Stenz, Katrine Tang Yan, Yan Sieljacks, Peter Wang, Jakob Groennebaek, Thomas Skjaerlund Jakobsgaard, Jesper Emil Rindom, Emil Herskind, Jon Gravholt, Anders Lassen, Thomas Ravn Jørgensen, Mathias Bæk, Rikke Gutiérrez-Jiménez, Eugenio Iversen, Nina Kerting Rasmussen, Peter Mondrup Nyengaard, Jens Randel Jørgensen, Malene Møller de Paoli, Frank Bøtker, Hans Erik Kjems, Jørgen Vissing, Kristian Drasbek, Kim Ryun Int J Mol Sci Article Ischemic conditioning and exercise have been suggested for protecting against brain ischemia-reperfusion injury. However, the endogenous protective mechanisms stimulated by these interventions remain unclear. Here, in a comprehensive translational study, we investigated the protective role of extracellular vesicles (EVs) released after remote ischemic conditioning (RIC), blood flow restricted resistance exercise (BFRRE), or high-load resistance exercise (HLRE). Blood samples were collected from human participants before and at serial time points after intervention. RIC and BFRRE plasma EVs released early after stimulation improved viability of endothelial cells subjected to oxygen-glucose deprivation. Furthermore, post-RIC EVs accumulated in the ischemic area of a stroke mouse model, and a mean decrease in infarct volume was observed for post-RIC EVs, although not reaching statistical significance. Thus, circulating EVs induced by RIC and BFRRE can mediate protection, but the in vivo and translational effects of conditioned EVs require further experimental verification. MDPI 2022-03-19 /pmc/articles/PMC8951333/ /pubmed/35328755 http://dx.doi.org/10.3390/ijms23063334 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Article Gu, Tingting Just, Jesper Stenz, Katrine Tang Yan, Yan Sieljacks, Peter Wang, Jakob Groennebaek, Thomas Skjaerlund Jakobsgaard, Jesper Emil Rindom, Emil Herskind, Jon Gravholt, Anders Lassen, Thomas Ravn Jørgensen, Mathias Bæk, Rikke Gutiérrez-Jiménez, Eugenio Iversen, Nina Kerting Rasmussen, Peter Mondrup Nyengaard, Jens Randel Jørgensen, Malene Møller de Paoli, Frank Bøtker, Hans Erik Kjems, Jørgen Vissing, Kristian Drasbek, Kim Ryun The Role of Plasma Extracellular Vesicles in Remote Ischemic Conditioning and Exercise-Induced Ischemic Tolerance |
| title | The Role of Plasma Extracellular Vesicles in Remote Ischemic Conditioning and Exercise-Induced Ischemic Tolerance |
| title_full | The Role of Plasma Extracellular Vesicles in Remote Ischemic Conditioning and Exercise-Induced Ischemic Tolerance |
| title_fullStr | The Role of Plasma Extracellular Vesicles in Remote Ischemic Conditioning and Exercise-Induced Ischemic Tolerance |
| title_full_unstemmed | The Role of Plasma Extracellular Vesicles in Remote Ischemic Conditioning and Exercise-Induced Ischemic Tolerance |
| title_short | The Role of Plasma Extracellular Vesicles in Remote Ischemic Conditioning and Exercise-Induced Ischemic Tolerance |
| title_sort | role of plasma extracellular vesicles in remote ischemic conditioning and exercise-induced ischemic tolerance |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951333/ https://www.ncbi.nlm.nih.gov/pubmed/35328755 http://dx.doi.org/10.3390/ijms23063334 |
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