Cargando…

Reclassification of Enterobacter sp. FY-07 as Kosakonia oryzendophytica FY-07 and Its Potential to Promote Plant Growth

Precise classification of bacteria facilitates prediction of their ecological niche. The genus Enterobacter includes pathogens of plants and animals but also beneficial bacteria that may require reclassification. Here, we propose reclassification of Enterobacter FY-07 (FY-07), a strain that has many...

Descripción completa

Detalles Bibliográficos
Autores principales: Gao, Ge, Zhang, Yan, Niu, Shaofang, Chen, Yu, Wang, Shaojing, Anwar, Nusratgul, Chen, Shuai, Li, Guoqiang, Ma, Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951479/
https://www.ncbi.nlm.nih.gov/pubmed/35336150
http://dx.doi.org/10.3390/microorganisms10030575
_version_ 1784675396601511936
author Gao, Ge
Zhang, Yan
Niu, Shaofang
Chen, Yu
Wang, Shaojing
Anwar, Nusratgul
Chen, Shuai
Li, Guoqiang
Ma, Ting
author_facet Gao, Ge
Zhang, Yan
Niu, Shaofang
Chen, Yu
Wang, Shaojing
Anwar, Nusratgul
Chen, Shuai
Li, Guoqiang
Ma, Ting
author_sort Gao, Ge
collection PubMed
description Precise classification of bacteria facilitates prediction of their ecological niche. The genus Enterobacter includes pathogens of plants and animals but also beneficial bacteria that may require reclassification. Here, we propose reclassification of Enterobacter FY-07 (FY-07), a strain that has many plant-growth-promoting traits and produces bacterial cellulose (BC), to the Kosakonia genera. To re-examine the taxonomic position of FY-07, a polyphasic approach including 16S rRNA gene sequence analysis, ATP synthase β subunit (atpD) gene sequence analysis, DNA gyrase (gyrB) gene sequence analysis, initiation translation factor 2 (infB) gene sequence analysis, RNA polymerase β subunit (rpoB) gene sequence analysis, determination of DNA G + C content, average nucleotide identity based on BLAST, in silico DNA–DNA hybridization and analysis of phenotypic features was applied. This polyphasic analysis suggested that Enterobacter sp. FY-07 should be reclassified as Kosakonia oryzendophytica FY-07. In addition, the potential of FY-07 to promote plant growth was also investigated by detecting related traits and the colonization of FY-07 in rice roots.
format Online
Article
Text
id pubmed-8951479
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89514792022-03-26 Reclassification of Enterobacter sp. FY-07 as Kosakonia oryzendophytica FY-07 and Its Potential to Promote Plant Growth Gao, Ge Zhang, Yan Niu, Shaofang Chen, Yu Wang, Shaojing Anwar, Nusratgul Chen, Shuai Li, Guoqiang Ma, Ting Microorganisms Article Precise classification of bacteria facilitates prediction of their ecological niche. The genus Enterobacter includes pathogens of plants and animals but also beneficial bacteria that may require reclassification. Here, we propose reclassification of Enterobacter FY-07 (FY-07), a strain that has many plant-growth-promoting traits and produces bacterial cellulose (BC), to the Kosakonia genera. To re-examine the taxonomic position of FY-07, a polyphasic approach including 16S rRNA gene sequence analysis, ATP synthase β subunit (atpD) gene sequence analysis, DNA gyrase (gyrB) gene sequence analysis, initiation translation factor 2 (infB) gene sequence analysis, RNA polymerase β subunit (rpoB) gene sequence analysis, determination of DNA G + C content, average nucleotide identity based on BLAST, in silico DNA–DNA hybridization and analysis of phenotypic features was applied. This polyphasic analysis suggested that Enterobacter sp. FY-07 should be reclassified as Kosakonia oryzendophytica FY-07. In addition, the potential of FY-07 to promote plant growth was also investigated by detecting related traits and the colonization of FY-07 in rice roots. MDPI 2022-03-06 /pmc/articles/PMC8951479/ /pubmed/35336150 http://dx.doi.org/10.3390/microorganisms10030575 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gao, Ge
Zhang, Yan
Niu, Shaofang
Chen, Yu
Wang, Shaojing
Anwar, Nusratgul
Chen, Shuai
Li, Guoqiang
Ma, Ting
Reclassification of Enterobacter sp. FY-07 as Kosakonia oryzendophytica FY-07 and Its Potential to Promote Plant Growth
title Reclassification of Enterobacter sp. FY-07 as Kosakonia oryzendophytica FY-07 and Its Potential to Promote Plant Growth
title_full Reclassification of Enterobacter sp. FY-07 as Kosakonia oryzendophytica FY-07 and Its Potential to Promote Plant Growth
title_fullStr Reclassification of Enterobacter sp. FY-07 as Kosakonia oryzendophytica FY-07 and Its Potential to Promote Plant Growth
title_full_unstemmed Reclassification of Enterobacter sp. FY-07 as Kosakonia oryzendophytica FY-07 and Its Potential to Promote Plant Growth
title_short Reclassification of Enterobacter sp. FY-07 as Kosakonia oryzendophytica FY-07 and Its Potential to Promote Plant Growth
title_sort reclassification of enterobacter sp. fy-07 as kosakonia oryzendophytica fy-07 and its potential to promote plant growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951479/
https://www.ncbi.nlm.nih.gov/pubmed/35336150
http://dx.doi.org/10.3390/microorganisms10030575
work_keys_str_mv AT gaoge reclassificationofenterobacterspfy07askosakoniaoryzendophyticafy07anditspotentialtopromoteplantgrowth
AT zhangyan reclassificationofenterobacterspfy07askosakoniaoryzendophyticafy07anditspotentialtopromoteplantgrowth
AT niushaofang reclassificationofenterobacterspfy07askosakoniaoryzendophyticafy07anditspotentialtopromoteplantgrowth
AT chenyu reclassificationofenterobacterspfy07askosakoniaoryzendophyticafy07anditspotentialtopromoteplantgrowth
AT wangshaojing reclassificationofenterobacterspfy07askosakoniaoryzendophyticafy07anditspotentialtopromoteplantgrowth
AT anwarnusratgul reclassificationofenterobacterspfy07askosakoniaoryzendophyticafy07anditspotentialtopromoteplantgrowth
AT chenshuai reclassificationofenterobacterspfy07askosakoniaoryzendophyticafy07anditspotentialtopromoteplantgrowth
AT liguoqiang reclassificationofenterobacterspfy07askosakoniaoryzendophyticafy07anditspotentialtopromoteplantgrowth
AT mating reclassificationofenterobacterspfy07askosakoniaoryzendophyticafy07anditspotentialtopromoteplantgrowth