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Preparation and Characterization of Doxycycline-Loaded Electrospun PLA/HAP Nanofibers as a Drug Delivery System

The present study aimed to prepare nanofibers by electrospinning in the system polylactic acid-hydroxyapatite-doxycycline (PLA-HAP-Doxy) to be used as a drug delivery vehicle. Two different routes were employed for the preparation of Doxy-containing nanofibers: Immobilization on the electrospun mat’...

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Autores principales: Farkas, Noémi-Izabella, Marincaș, Laura, Barabás, Réka, Bizo, Liliana, Ilea, Aranka, Turdean, Graziella Liana, Toșa, Monica, Cadar, Oana, Barbu-Tudoran, Lucian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951507/
https://www.ncbi.nlm.nih.gov/pubmed/35329557
http://dx.doi.org/10.3390/ma15062105
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author Farkas, Noémi-Izabella
Marincaș, Laura
Barabás, Réka
Bizo, Liliana
Ilea, Aranka
Turdean, Graziella Liana
Toșa, Monica
Cadar, Oana
Barbu-Tudoran, Lucian
author_facet Farkas, Noémi-Izabella
Marincaș, Laura
Barabás, Réka
Bizo, Liliana
Ilea, Aranka
Turdean, Graziella Liana
Toșa, Monica
Cadar, Oana
Barbu-Tudoran, Lucian
author_sort Farkas, Noémi-Izabella
collection PubMed
description The present study aimed to prepare nanofibers by electrospinning in the system polylactic acid-hydroxyapatite-doxycycline (PLA-HAP-Doxy) to be used as a drug delivery vehicle. Two different routes were employed for the preparation of Doxy-containing nanofibers: Immobilization on the electrospun mat’s surface and encapsulation in the fiber structure. The nanofibers obtained by Doxy encapsulation were characterized using Fourier transform infrared (FTIR) spectroscopy, thermogravimetric (TG) and differential thermal analyses (DTA) and scanning electron microscopy (SEM). The adsorption properties of pure PLA and PLA-HAP nanofibers were investigated for solutions with different Doxy concentrations (3, 7 and 12 wt%). Moreover, the desorption properties of the active substance were tested in two different fluids, simulated body fluid (SBF) and phosphate buffer solution (PBS), to evidence the drug release properties. In vitro drug release studies were performed and different drug release kinetics were assessed to confirm the use of these nanofiber materials as efficient drug delivery vehicles. The obtained results indicate that the PLA-HAP-Doxy is a promising system for biomedical applications, the samples with 3 and 7 wt% of Doxy-loaded PLA-HAP nanofibers prepared by physical adsorption are the most acceptable membranes to provide prolonged release in PBS/SBF rather than an immediate release of Doxy.
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spelling pubmed-89515072022-03-26 Preparation and Characterization of Doxycycline-Loaded Electrospun PLA/HAP Nanofibers as a Drug Delivery System Farkas, Noémi-Izabella Marincaș, Laura Barabás, Réka Bizo, Liliana Ilea, Aranka Turdean, Graziella Liana Toșa, Monica Cadar, Oana Barbu-Tudoran, Lucian Materials (Basel) Article The present study aimed to prepare nanofibers by electrospinning in the system polylactic acid-hydroxyapatite-doxycycline (PLA-HAP-Doxy) to be used as a drug delivery vehicle. Two different routes were employed for the preparation of Doxy-containing nanofibers: Immobilization on the electrospun mat’s surface and encapsulation in the fiber structure. The nanofibers obtained by Doxy encapsulation were characterized using Fourier transform infrared (FTIR) spectroscopy, thermogravimetric (TG) and differential thermal analyses (DTA) and scanning electron microscopy (SEM). The adsorption properties of pure PLA and PLA-HAP nanofibers were investigated for solutions with different Doxy concentrations (3, 7 and 12 wt%). Moreover, the desorption properties of the active substance were tested in two different fluids, simulated body fluid (SBF) and phosphate buffer solution (PBS), to evidence the drug release properties. In vitro drug release studies were performed and different drug release kinetics were assessed to confirm the use of these nanofiber materials as efficient drug delivery vehicles. The obtained results indicate that the PLA-HAP-Doxy is a promising system for biomedical applications, the samples with 3 and 7 wt% of Doxy-loaded PLA-HAP nanofibers prepared by physical adsorption are the most acceptable membranes to provide prolonged release in PBS/SBF rather than an immediate release of Doxy. MDPI 2022-03-12 /pmc/articles/PMC8951507/ /pubmed/35329557 http://dx.doi.org/10.3390/ma15062105 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Farkas, Noémi-Izabella
Marincaș, Laura
Barabás, Réka
Bizo, Liliana
Ilea, Aranka
Turdean, Graziella Liana
Toșa, Monica
Cadar, Oana
Barbu-Tudoran, Lucian
Preparation and Characterization of Doxycycline-Loaded Electrospun PLA/HAP Nanofibers as a Drug Delivery System
title Preparation and Characterization of Doxycycline-Loaded Electrospun PLA/HAP Nanofibers as a Drug Delivery System
title_full Preparation and Characterization of Doxycycline-Loaded Electrospun PLA/HAP Nanofibers as a Drug Delivery System
title_fullStr Preparation and Characterization of Doxycycline-Loaded Electrospun PLA/HAP Nanofibers as a Drug Delivery System
title_full_unstemmed Preparation and Characterization of Doxycycline-Loaded Electrospun PLA/HAP Nanofibers as a Drug Delivery System
title_short Preparation and Characterization of Doxycycline-Loaded Electrospun PLA/HAP Nanofibers as a Drug Delivery System
title_sort preparation and characterization of doxycycline-loaded electrospun pla/hap nanofibers as a drug delivery system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951507/
https://www.ncbi.nlm.nih.gov/pubmed/35329557
http://dx.doi.org/10.3390/ma15062105
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